Samples to check the quality of disinfection

of used instruments.
 Quality control of disinfection.
 The quality of disinfection is judged by the absence of Staphylococcus aureus, Pseudomonas aeruginosa
and E.coli bacteria on medical products after it has been carried out. Control is subject to 1% of
simultaneously processed products of the same name (but not less than 3 units).
 The quality control of disinfection is carried out by the wash-down method. Swabs are taken from the
surface of medical devices before and after disinfection. The swabs are taken with sterile gauze wipes
soaked in sterile water.
 Washes of 0.1 ml are applied on the surface of yolk-salt, blood agar and on the Endo medium. Crops are
kept in a thermos at 37 ° C. The results are taken into account after 48 hours. In the presence of the growth
of microorganisms on agar, identification with the isolated microorganism is carried out in accordance
with the current methodological documents. Disinfection is considered effective in the absence of
microbial growth.
Quality control of pre-sterilization cleaning.
 Quality control of pre-sterilization cleaning is carried out by setting
azopyram or amidopyrine samples for the presence of blood residues
and phenolphthaleic ones for the presence of residual amounts of
alkaline components of the detergent.
 Azopyram test.

 Method of checking how well the pre-sterilization cleaning of instruments was carried out.
 It is carried out to determine residues, foreign traces of microscopic fragments of blood,
traces of rust, detergent and other elements on instruments.
 Azopyram stock solution is prepared in a pharmacy and stored in the refrigerator for up to
2 months.
 The working solution of azopyram is prepared immediately before use by mixing the basic
solution of azopyram and a 3% solution of hydrogen peroxide in a 1: 1 ratio, stored for no
more than 2 hours.
 Azopyram test.
 The reagent should not be placed near heating devices and in bright light.
 Sample preparation must be carried out on cold instruments.
 The solution is applied to the cotton wool to wipe the tool.
 With a positive test in the presence of traces of blood, a purple coloration appears immediately or no later
than 1 minute later, within a few seconds, turning into a pink-lilac or brownish color (brownish if there is
rust on the instruments).
 In case of a positive result for blood, the entire batch of instruments must be re-cleaned, namely the stage
of thorough washing with tampons in a washing solution.
 After checking, it is necessary to remove the remains of azopyram from the examined products by
washing them with water or wiping them with tampons moistened with water or alcohol, and then repeat
the pre-sterilization cleaning of these products.
 The test with azopyram is 10 times more sensitive than the amidopyrine test.
False positive reaction of the azopyram test.

 If you wait for the result for more than 1 minute.

 If a rusty (with traces of corrosion) instrument appears (brown staining).
 When checking a hot tool.
 When storing the reagent in bright light and near heating appliances.
 When using the reagent over the specified storage time.
 Amidopyrine test.
 Before setting the sample in the crucible, mix equal amounts of 5% alcohol solution of
amidopyrine, 30% solution of acetic acid and 3% solution of hydrogen peroxide.
 The appearance of a blue-green color when the reagent is applied to the product indicates
the presence of blood residues.
 The reagent is checked for occult blood in the same way as in the previous case.
 In case of a positive reaction to occult blood, the entire batch of products is again subjected
to repeated processing.
 Inspection is daily subjected to 3% of simultaneously processed products of the same
purpose (batch), but not less than 3-5 samples.
Phenolphthalein test.

 Determines the presence of residual amounts of alkaline components of detergents.

 A 1% alcoholic solution of phenolphthalein is prepared.
 1 g of phenolphthalein per 100 ml of 95% alcohol.
 The shelf life of the solution is unlimited.
 1-2 drops of the solution are applied to the washed product; in the presence of detergents, a
pink color appears.
 If the result is positive, the entire batch must be rinsed again under running water, and then
with distilled water.
Sterilization quality control.

 Monitoring of work involves checking the parameters of the sterilization modes and assessing its
effectiveness.
 The control of the parameters of the sterilization modes is carried out by physical (using
instrumentation) and chemical (using chemical indicators) methods.
 The effectiveness of sterilization is assessed on the basis of the results of bacteriological studies
carried out using biotests and monitoring the sterility of products subjected to sterilization.
 Quality control of sterilization of medical devices is carried out by checking the temperature regime.
The temperature regime is checked using thermometers.
 Chemical indicators are also used to control temperature. Indicators are a strip of paper with an
indicator layer applied to it and are intended for operational visual control of the set of parameters
(temperature, time) of the operating modes of steam and air sterilizers.