USE OF PHYTASE TREATED CORN DISTILLER’S DRIED

GRAINS WITH SOLUBLES IN CHINESE CATFISH (Silurus

asotus) DIET
April 30 – May 05, 2013

Table 2: Main and interaction effect of phytase

inclusion method (P.I.M) and level (P.I.L)on growth
Abstract Materials and methods \
and survival of Chinese catfish

Chinese catfish (Silurus asotus)

This study determined the effects of diets
containing corn distiller’s dried drains with
solubles treated with three phytase levels by two
methods on growth, nutrients digestibility and
whole body composition in Chinese catfish.
Phytase was included at 0, 1000 and 5000 U kg -
1
diet by post-spray (PS0, PS1, PS5), spray on
made diet, or by pretreatment (PT0, PT1, PT5),
treat DDGS with phytase before making diets. Table 3: Main and interaction effect of phytase
Cr2O3 at 1% was added to the diets as an inert inclusion method and level on whole body
marker for the digestibility study conducted for 3 composition and protein utilization of Chinese
weeks after a 10-week growth experiment. Fish catfish
(25.01±0.16 g) were stocked in triplicate groups
of 8 fish per cage (800 fish/m3) and fed to
apparent satiation twice daily. After 10 weeks,
pretreatment method (PT) had higher survival
rate (SR) and yield than post-spray (PS). Fish
fed phytase treated diets had higher final body
weight, weight gain, specific growth rate (SGR)
and feed intake (FI) than the control fish. Feed
conversion ratio (FCR) and survival rate were
not affected by the phytase level. SGR had Table 4: Main and interaction effect of phytase
positive correlations with the yield and FI. The inclusion method and level on Apparent
latter had also a positive relationship with yield, Digestibility Coefficients (ADCs %) of dry matter,
FCR and SR. Fish in the PT group had higher Pretreatment (PT) and post-spray (PS) crude protein, lipid, ash and Phosphorus of
body crude protein (CP), ash and phosphorus
Chinese catfish
(P) content and apparent digestibility Dissolution of
phytase (PT)
coefficients (ADCs) of the same elements than
PS. However crude lipid was not affected.
Generally, phytase improved the ADCs of dry
matter (DM) and Increasing phytase level also
increased both ADCs and fish body content of
Buffer solution pH 5
CP, ash and P. The highest phytase level at room temperature Incubation at 50-55°C for 5-
6 h in oven
enhanced the protein efficiency ratio (PER), Dissolution of phytase (PS)
protein growth rate (PGR) and protein Cooling and drying for 24h
and make the diets
productive value (PPV). ADCs of nutrients were
positively correlated with both body content of
the same elements and the protein utilization
Control diet>>> with distilled Conclusions
water
parameters. The use of phytase in DDGS in diet
1. Phytase improved growth, ADCs and whole
enhanced growth performance, protein
utilization and the ADCs of CP, ash and P in body composition of nutrients.
Chinese catfish. Consequently, the nutrient load
Results 2. The protein utilization was enhanced by phytase
in environment, especially P, would be treatment
minimized. The pretreatment is more effective in
Table 1: Proximate analysis of the experimental
our study than the post- spray. 3. Pretreatment is a better choice than the post
diets spray when phytase is used in Chinese catfish
Introduction diet.
Diet
PS0 PS1 PS5 PT0 PT1 PT5 4.Optimum level should lie between 1000 and
1. Chinese catfish is mainly disributed in notation
5000 U/kg diet
the freshwater of china, Japan (Wen and
Diet
Lin, 2004) P.I.M1 PS PS PS PT PT PT
ingredients Acknowledgments
2. Replacement of fishmeal by alternative P.I.L2 Fish 1. International Cooperation and Development
plant ingredients has been the focus on 0 1000 5000 0 1000 5000 DDGS 3

(U kg-1) meal Fund (ICDF, Taiwan)

feed formulation
3. Plant proteins contain some anti- Proximate4 2. Department of Aquaculture, National Taiwan
nutrients like phytate which can chelate Moisture Ocean University.
mainly phosphorus et important minerals 13.33 13.08 12.86 11.90 11.70 12.66 9.57 11.12
(%) References
to cause environnemental pollution
 
4. About 65% of total phosphorus in corn DM (%) 86.67 86.92 87.14 88.10 88.30 87.34 90.43 88.88 1. Noureddini, H.,Malik, M., Byun, J.,Ankeny,
DDGS is phytic acid bound phosphorus
A.J., 2009. Distribution of phosphorus compounds
(Nourreddini et al., 2009)
in corn processing. Bioresource Technology 100,
5. The use of phytase has been proven to CP (%) 34.30 34.10 34.01 34.32 34.39 34.45 27.47 64.38
731-736
unlock the chelated minerals and
2. Sajjadi, M.,Carter, C.G, 2004. Dietary phytase
improve their absorption; however few
CL (%) 10.21 10.27 10.30 10.83 10.86 10.92 10.31 8.85 supplementation and the utilisation of phosphorus
studies have been ever conducted by
by Atlantic salmon (Salmo salar L.) fed a canola
adding phytase in diets with different
meal-based diet. Aquaculture 240, 417-431
inclusion methods (Sajjadi and Carter, 2004) Ash (%) 8.42 8.74 8.74 8.46 9.81 9.87 5.32 15.53
3. Wen,H.S., Lin, H.R., 2004. Effects of exogenous
6. This study aimed to determine the
neurohormone,gonadotropin(GtH) and
effects of three phytase supplemental Total P
3.32 3.81 3.87 3.30 3.57 3.99 9.40 13.6 dopaminergic drugs on the serum GtH content and
levels by two inclusion methods in diets (g/kg) ovulatory responsiveness of wild catfish, Silurus
containing DDGS for Chinese catfish
GE5 asotus (Linnaeus,1758).Aquaculture Research 35,
17.75 18.17 18.25 18.21 18.21 18.22 ND ND 204-212.
(MJ/kg)