Define culture sel

Cell culture is a technique that is widely used in the study of

human metabolism and human physiology which is not easy to
perform in vivo. Cells can be isolated from tissue, then cultured
for days to weeks.
Usable Cells
1. normal cell tissue ( skin tissue)
2. diseased tissue (liver tumor cells)
Requirements for Cultivable Cells
Cells used for culture are usually taken from eukaryotic tissue
For example:
In vitro
1. epithelial cells cell epitelial-like (cell HeLa, cell Vero)
2. fibroblast cells In vitro cell fibroblast-like (en:lung mice fibroblast (LMF)
3. lymphoblast cells In vitro cell Raji

Types of Media That Can Be Used

4. EMEM formulated by Harry Eagle from the earlier and simpler basal medium (BME)
5. DMEM a modification of the Basal Medium Eagle (BME)
6. RPMI-1640 modified McCoy's 5A and developed for long-term culture of peripheral blood lymphocytes.
7. DMEM/F12 a mixture of DMEM and Ham’s F-12
8. Ham’s F12-K mediums such as EMEM, there are various formulation modifications including Ham's
F-12 medium, a more complex formulation suitable for serum-free culture.