Serum samples collection and preparation

stand for 10 min centrifugation at 4000 rpm Resultant supernatant layers

at room temperature (4 0C for 10 min) 1.5 mL tubes and stored at -
80 C until analysis

Blood samples (3
mL)
thawed overnight at 4 C
20 μL of each sample was Afte mixing
transferred to its gentle mixing using
Afte mixing respective pre-cleaned 15 micropipette
mL polypropylene tube

360 μL of concentrated placed onto a heat block digester the samples were diluted
HNO3:HCl (1:1, v/v) with set temperature at 90–95 C with 2 mL of DI water
and 2000 μg/L ISTD for 2–3 h until the samples
turned clear

dilution into five-fold and 20-fold dilutions

each prior to the analysis by ICP-MS.
 Reagents and standard solutions
• Deionized (DI) water (18.2 MΩ)
• Seronorm® Trace Elements Serum Level 1 and 2
• HNO3
• HCI

Materials and Equipment

• Deionized (DI) water system (18.2 MΩ)
• Lab balance
• Glassware
• pHmetre
• conductivity meter

• Heat block