ESTIMATION OF Ni+2 BY BACK TITRATION METHOD

Aim: To estimate the amount of Ni+2 by

using back titration method.

Apparatus: 1.Conical flask

2.Beakers 3.Funnels 4.Burette
5.Pippete 6.Measuring jars
7.Waterbath 8.Whatman filterpaper.
Chemicals :
1.EDTA solution
2.pH = 10 Buffer solution
3. EBT indicator
4.MgSO4 solution
5. Ni+2 solution
Principle:
Back Titrations:
1. This is method, an excess of standard EDTA is added
to the sample solution of metal ion.
2. The resulting solution will contain unreacted EDTA
which is then back titrated with standard metal ion
solution in the presence of indicator.
3. ZnCl2 , ZnSO4 , MgCl2 , MgSO4 is used as standard
metal ion solution.
4.Al+3, Co+2, Pb+2 , Mn+2 , Hg+2 , and Ni+2 can be determined
by using Back titration method.
PRINCIPLE:
pH = 10 buffer
Ni+2+ EDTA-2 [ Ni– EDTA]
Procedure:
In the detemination of Ni+2 following three
are involved.
1. Preparation of standard
MgSO4 .7H2Osolution.
2. Standadisation of given EDTA solution.
3.Estimation of Ni+2 solution
1. Preparation of standard MgSO4 .7H2Osolution.
0.01M MgSO4 .7H2O solution is prepared by
dissolving 0.246g of MgSO4 in 100ml water.
Wt 1000
M= X
M.Wt Vml

M XM.Wt XVml
Wt =
1000

0.01 X246 X100 =0.246g

Wt =
1000
 2. Standadisation of given EDTA
1.The Burette
solution. is filled with given EDTA solution.
2.10ml of standard MgSO4 solution is Pipetted out
and transferred in to a clean conical flask.
3.Then 5 ml of pH=10 buffer and 3 drops of EBT
indicator is added ,solution changed to violet colour .
4.Then it is titrated with the EDTA solution taken in
the Burette till the colour changed to blue.
5.This is repeated till the concurrent values are
 3. Estimation of Ni+2.
1.10ml of given Ni+2 solution is pipetted out into a
Clean conical flask.
2.Then to it 20ml of EDTA solution is added.
the Burette till the colour changed to blue.
3.Then 10 ml of pH=10 buffer and 4 drops of
EBT
indicator
4.Then isistitrated
added ,solution changed
with standard to blue
MgSO 4 from the

colour . till the solution changed to wine red

burette
5.This process is repeated till the
concurrent values are obtained.
 Standardisation of EDTA solution (Step 2).
Total volume of EDTA
Burette Reading
Volume of MgSO4 solution.
S.No.
solution
Intial (V1) Final(V2) Final(V2)-Intial (V1)

1 10ml 0 8 8 ml
2 10ml 8 16 8 ml
3 10ml 16 24 8 ml
M1V1/n1=M2V2/n2

M1=Molarity of MgSO4 solution=0.01M M2=Molarity of EDTA solution=?

V1=Volume of MgSO4 solution=10ml V2=Volume of EDTA solution=8ml
n1=Number of moles of MgSO4 n2=Number of moles of MgSO4
solution=1 solution=1

M1 XV1 Xn2 0.01 X10 X1

M2 = = =0.0125M
n1 X V2 1X8
 Estimation of Ni+2 solution (Step 3).
           

Volume of Ni+2 Volume of Volume of MgSO4 solution used

S.No. Burette Reading
solution EDTA solution to titrate EDTA solution.

1 10ml 20ml 0 5 5 ml
2 10ml 20ml 5 10 5ml
3 10ml 20ml 10 15 5 ml

From Step 2 & Step 3

8 ml MgSO4 ------- Uses 10ml EDTA solution
5 ml MgSO4 -------- ?(How much EDTA is
used?)
= 10 X 5 /8 =6.25 ml

So volume of EDTA reacted with 10ml Ni+2

solution is = 20 – 6.25 =13.75ml
 M3V3/n3=M2V2/n2

M3=Molarity of Ni+2solution=? M2=Molarity of EDTA solution=0.0125

V3=Volume of Ni+2 solution=10ml M
V2=Volume of EDTA solution= 13.75 ml
n3=Number of moles of Ni+2
solution=1 n2=Number of moles of EDTA solution=1

M2 XV2 Xn3 0.0125 X13.75 X1

M3 = = =0.0171875M
n2 X V3 1 X 10

M 3XM.Wt XVml 0.0171875 X58.69 X100

Wt = Wt = =0.100873g
1000 1000

RESULT:
100 ml of given solution contains 0.100873 g
Of Ni+2