GENERAL MICROBIOLOGY LABORATORY

Isolation TECHNIQUES
MODULE 7
 TOPIC LEARNING OUTCOMES

At the end of the activity, the students are expected to:

- Perform streak, pour and spread plate techniques for the
isolation of microorganisms.
- Describe the distribution of colonies from each plating
method.
- Characterize types of isolates that can be grown from each
technique.
 BACTERIA EXIST IN MIXED
POPULATIONS.

• In order to adequately study and

characterize an individual bacterial species,
one needs a pure culture.
 PURE CULTURE
• Bacterial suspension consisting of a single
species.
 TECHNIQUES

1. Streak Plating
2. Spread Plating
3. Pour Plating
 PROCEDURES
1. Streak Plating
2. Spread Plating
3. Pour Plating
 STREAK PLATE METHOD

Mixed Culture NA plate

Inoculating needle
Streak Plating Method
Streak Plating Method
Streak Plating Method
Streak Plate Method
After incubation
SPREAD PLATING METHOD
SPREAD PLATING METHOD
POUR PLATING METHOD
POUR PLATING METHOD
 REFERENCES
Tortora, G.J., Funke, B.R. and Case, C.L. 2004. Microbiology: An
Introduction, 8 edition. California, USA : Pearson Education, Inc. /
th

Benjamin Cummings