Professor, Dept. of Microbiology, King George’s Medical University What is taxonomy • Classification: Orderly arrangement and placement of bacteria based on certain characters ie. Address
• Nomenclature: giving Names
• Families - related bacteria eg. Enterobacteriaceae • Within a family - numerous genus eg. Escherichia, Klebsiella, Salmonella • A genus contains many species eg. E. coli, E. tarda Species • Taxonomical unit • Based on morpholopgy, biochemical properties & antigenic characters • Nucleotide base ratio constant for a given species (AT : GC) • Genetic homology demonstrated by DNA hybridization > 98% Interspecies Classification They are identical, may show some differences due to mutation •Biotype - based on biochemical reactions •Serotype - based on antigens on bacteria •Phage type - susceptibility to particular phage •Colicin type - substance secreted by some bacteria •Molecular typing: Plasmid profile, DNA sequence • A bacterial population derived from a single bacteria by binary fission is called CLONE • A bacterial population obtained from a particular source is called STRAIN Nomenclature • Casual name / Scientific name: eg. Gonococcus / Neisseriae gonorrhoeae Pneumococcus / Streptococcus pneumoniae • Name could be a character: Staphylococcus aureus • Place of discovery: Salmonella london • Scientist: Clostridium welchii • Disease: Clostridium tetani Bacterial Growth & Metabolism Prokaryotes • Asexual reproduction - binary fission • Generation time or doubling time • Clinical implication? Bacterial Growth Curve 4 phases What is a Bacterial colony?