MORPHOLOGICAL CHANGES IN THE PULMONARY TISSUE OF MIDLIFE RATES UNDER CONDITIONS OF EXPERIMENTAL FIRST TYPE DIABETES

Teslyk T.P.
scientific supervisor - doctor of medical sciences, prof. Sikora V.Z.
Sumy State University, Medical Institute, Department of Morphology

Objective. Detect and explore the morphological lungs in conditions of aloxane diabetes .
Materials and methods. Three groups of white non-breeding rats of both sexes were studied, with terms of diabetes 10 days, 20 days, 30 days. Morphological indices
of animals in each experimental group were compared with those in intact animals. The following research methods were used: scanning electron microscopy
(micromorphomeria of conduction bronchioles width (CBW)), blood glucose levels determined by glucose oxidase method, glycosylated hemoglobin level (HbA1C).
M i c r o m o r p h o m e t r i c c h a r a c t er i s t i c s o f c o n -
1 2 3 d uc t i o n b r o nc h i o l e s w i d t h o f m i d l i f e r a t e s
und e r c o ndi t i o ns o f e x p e r i m e nt a l d i a b et e s
c o m pa r e d t o i nt a c t g r up s

72
70.15
70
68 67.3

66 65.52

mkm
64.4
63.53 63.7
64
62
60
10 days DM 20 days DM 30 days DM
Lung tissue of young rats in conditions of alloxan diabetes for 10 (1), 20 (2), 30(3) days. Scanning electron demonstration intact groups
microscopic photography. ×1200, 1500, 800. demonstration of rats whith diabetes mellitus

At 10 days indicators of glucose in the blood and glycosylated hemoglobin were 14.8 ± 0.19 mmol /l and 6.3 ± 0.14% (p <0.05) respectively, corresponding to a
severe degree of diabetes mellitus.At 20 days the average glucose indexes in the blood and glycosylated hemoglobin was 12.3 ± 0.04 mmol /l and 7.1 ± 0.14% (p
<0.05) respectively, which corresponds to the average level of diabetes mellitus. At 30 days blood glucose and glycosylated hemoglobin values were 10.2 ± 0.1
mmol/l and 7.4 ± 0.08% (p <0.05) respectively, which corresponds to the average degree of diabetes mellitus.

Conclusions. Under the conditions of experimental aloxane diabetes, which corresponded to severe and moderate stages (from 10 to 30 days), dilatation changes in
leading bronchioles develop in rats of mature age, which may indicate of initial changes in the pulmonary stroma of the emphysematous direction.