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KELOMPOK 1

Passive voice
&
Reguller verb and
Irreguler verb
KELOMPOK 1
ELVIRA HENDINI (18033048)
DEWI RATNA SARI (18033046)
NOVIA AGUSTIN (18033050)
NURUL HASANAH PARNI (18033051)
NISIKA TASIRILELEU (20033025)
DESTY MELINIA (18033007)
DOSEN PEMBIMBING:
Yahdi Bin Rus, S.Pd., M.Si., Ph.D
KELOMPOK 1

1 PASIVE VOICE
2 VOCABULARY
3 IRREGULAR VERB
4 REGULAR VERB
KELOMPOK 1
PASSIVE VOICE

CorvGenSurv’s precision was tested by comparing the


consensus sequence from a given number of reads.
(Presisi CorvGenSurv diuji dengan membandingkan urutan
konsekuensi dari sejumlah pembacaan tertentu).

Taste-Tasted-Tasted
KELOMPOK 1
PASSIVE VOICE

This accuracy was achieved by our error correction step during consensus
sequence building.

(Keakuratan ini dicapai dengan langkah koreksi kesalahan kami selama pembuatan
urutan konsensus)

Achieve- achieve- achieved


RT-PCR was performed using the Roche COBAS system.
(qRT-PCR dilakukan menggunakan sistem Roche COBAS).
Perform-performed-performed

We reported a total of nine new amino acid mutations that were


not observed among 28,176 global SARS-CoV-2 sequences in
GISAID.
(Kami melaporkan total sembilan mutasi asam amino baru yang tidak
diamati di antara 28.176 sekuens SARS-CoV-2 global di GISAID)
Observe- observed- observed
We observed that 31 or more reads would be required to
produce an accurate consensus sequence.
(Kami mengamati bahwa 31 bacaan atau lebih akan diperlukan untuk
menghasilkan urutan konsensus yang akurat.)
require- required - required

60 reads per consensus sequence can be obtained on average


to recover a minimum of 31 reads per consensus sequence.
(60 bacaan per urutan konsensus dapat diperoleh rata-rata untuk memulihkan
minimum 31 bacaan per urutan konsensus)

(Obtain - Obtained - Obtained)


Infuenza has also been shown to escape from vaccine-induced immunity.
Infuenza telah terbukti lolos dari imunitas akibat vaksin.
(Show-Shown- shown)

The nucleotide distance among all pairs of sequences was scaled to the
Euclidean distance by multidimensional scaling.
Jarak nukleotida di antara semua pasangan urutan adalah skala ke jarak Euclidean oleh
skala multidimensi.
(Scale - scaled - scaled)
Thus SARS-CoV-2 evolution must be continuously monitored in real time
to prevent vaccine failure and guide future vaccine strain selection.
(Jadi evolusi SARS-CoV-2 harus terus dipantau secara langsung untuk mencegah
kegagalan vaksin dan memandu pemilihan strain vaksin di masa depan)

( Monitor - monitored - monitored)

These specimens were collected from Los Angeles County, California,USA


between April 13 th and june 22nd, 2020 (Table1).
(spesimen ini Dikumpulkan dariLos Angeles County, California.USAantara 13 April dan
Juni2020 (Tabel1).22nd)

collect- collected-collected
This study (HS-20-00326) was approved by the Institutional Review Board
of the University of Southern California as non-human subjects rescarch.

(Ini disetujui oleh Institutional ReviewDewan Universitas SouthernCalifornia sebagai


subjek non-manusia penelitian ulang (HS-20-00326)

Approve- approved- approved


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BEAST42 v.1.10.4 was used to estimate the rate of SARS-Co V-2

(BEAST42 v.1.10.4 digunakan untuk memperkirakan tingkat


SARS-CoV-2)
Use-used-used
REGULAR VERB

01
COVID-19 global cases have climbed
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to more than 33 million, with over a


million total deaths.

Kasus global COVID-19 telah meningkat


menjadi lebih dari 33 juta dengan lebih dari
satu juta total kematian

Climb-climbed-climbed
02
as observed in GISAID and Nextstrain,
may lower COVID-19 vaccine
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effectiveness as observed in influenza


vaccines.

seperti yang diamati pada GISAID dan Nextstrain dapat


menurunkan efektivitas vaksin COVID-19 seperti yang
diamati pada vaksin influenza.

Observe-observed-observed
03
We produced near full-length whole genome
sequences from individuals who were COVID-19
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test positive during April to June 2020 in Los


Angeles Counrty,California, USA.

Kami menghasilkan hampir seluruh urutan genom


sepanjang penuh dari individu uang dinyatakan positif
COVID-19 selama April hingga Juni 2020 di negara Los
Angeles , California, AS

Produce-produced-produced
04
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The median divergence time was


estimated to be December 5th 2019.
Waktu divergensi median diperkirakan pada 5
Desember 2019.

(Estimate - estimated - estimated)


05

60 reads per consensus sequence can be obtained


on average.

Rata-rata 60 pembacaan dapat diperoleh

(Obtain - obtained - obtained)


IRREGULAR VERB
01
Influenza has also been shown to escape from vaccine induced
immunity.

(Influenza juga telah terbukti lolos dari kekebalan yang terbukti lolos dari kekebalan
yang diinduksi vaksin.)

(Show - shown - shown)


These primers span the near full-length SARS-CoV-2 genom in
02 three segments with overlap for genome assembly.

(primer ini memutar genom SARS-CoV 2 tang hampir full-length


dalam 3 segmen dengan tumpang tindih untuk perakitan genom)
Spin-Span-Spun
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The samples PCR cycled with 25 cycles of 98°C for 10 s and


03 68°C for 10 min, held at 4°C until the next step

(sampel PCR disiklus dengan 25 siklus 98°C selama 10 detik dan


68 °C selama 10 menit. Ditahan pada suhu 4°C hingga langkah
selanjutnya)

Hold-Held-Held
When a consensus sequence was built from
three reads, only 67% [52.4–78.9%] of the 1000
bootstrap runs’ resulting consensus sequences
were consistent with the correct sequence.
04
Ketika urutan konsesus dibagun dari tiga
pembacaan,hanya 67 % [52.4–78.9%]
dari 1000 gantungan berjalan yang
menghasilkan urutan knsesus yang
konsisten dengan urutan yang benar.

Build- Built- Built


05.
The HA sequences in January 2020 showed greater nucleotide
distances from the 2019–2020 vaccine strain than those in April
2019 (p < 0.001, Wilcoxon rank sum test).

Urutan HA dalam Jarak nukleotida yang lebih besar dari galur vaksin 2019-
2020 dibandingkan pada April 2019 (p < 0.001,uji jumlah peringkat
Wilcoxon).

Show-showed-Shown
VOCABULARY

Viral = virus Amplified =


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Obtained = Pooled =
diperoleh dikumpulkan
VOCABULARY
Yields = hasil
Surveillance = pengawasan
Workflow = alur kerja
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Pompted = terpukau
•Sequence = urutan Enhance = menekankan
•During = selama Modulate = mengatur
•Crucial = penting Numerous = banyak
•Expanding = memperluas
•Efforts = upaya
•Surveillance = pengawasan
•Sequencing = rangkaian
THANKS
YOU

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