GROUP 1 8275L

LABORATORY
ACTIVITY 03
ANALYSIS OF BUFFER SYSTEMS
OBJECTIVES
This activity aims to:

Compare the buffering activity of

01 egg albumin and analytical grade

albumin.
Discuss the buffering activity of

02
molecules comprising in egg
albumin and analytical grade
albumin.
Cite the importance of buffer

03 mechanisms in biological systems

BUFFER SYSTEM
Whether inside your
body or not, it helps to
control the acidity and
alkalinity of a solution
(Rodwell, 2018) GROUP 1
AMINO
ACIDS
Composed of the following:
alpha carboxyl group, alpha-
amino group, and a side chain
called the R group

GROUP 1
 EXPERIMENTAL
TABLE OF MATERIALS AND REAGENTS
LIST OF EQUIPMENT
FLOWCHART
 MATERIALS REAGENTS

TABLE OF Whole uncooked egg 0.1N NaOH Solution

0.1N HCl Solution

MATERIALS
Albumin (analytical grade)

AND Distilled water

REAGENTS
250 mL beaker

Pipettes

GROUP 1
LIST OF
EQUIPMEN
pH meter
T

GROUP 1
Figure 1. Procedure for the analysis of buffer system
RESULTS AND
DISCUSSION
RESULTS WITH KNOWN
VALUES COMPARISON
Figure 2. Titration curve of distilled water with 0.1 N NaOH and 0.1 N HCl
INTRODUCTION
TO AMINO ACIDS
ACID-BASE
REACTION

GROUP 1
GLUTAMIC
ACID
R-GROUP:
NON-ALPHA COOH

pKa: 4.10-4.80

GROUP 1
ASPARTIC ACID
R-GROUP:
NON-ALPHA COOH

pKa: 3.90 - 4.80

GROUP 1
HISTIDINE
R-GROUP:
IMIDAZOLE

pKa: 6.50-7.40

GROUP 1
THREONINE
R-GROUP:
HYDROXYL

pKa: ∼13.00

GROUP 1
SERINE
R-GROUP:
HYDROXYMETHYL
GROUP

pKa: ∼13.00

GROUP 1
ARGININE
R-GROUP:
GUANIDINO GROUP

pKa: ∼12.00

GROUP 1
LYSINE
R-GROUP:
FIFTH CARBON AMINO
GROUP

pKa: 9.80-10.80

GROUP 1
CYSTEINE
R-GROUP:
THIOL GROUP

pKa: 8.50 - 9.00

GROUP 1
 EQUIVALENCE POINT
INITIAL POINT: 6.24

Figure 3. Titration curve of analytical grade albumin with 0.1 N HCl and 0.1 N NaOH
 ARGININE
pKa: ∼12.00
LYSINE
pKa: 9.4 - 10.8

EQUIVALENCE POINT
INITIAL POINT: 6.24

GLUTAMIC ACID
pKa: 4.1
&
ASPARTIC ACID
pKa: 3.90

Figure 3.1. Titration curve of analytical grade albumin with 0.1 N HCl and 0.1 N NaOH
 EQUIVALENCE POINT
INITIAL PH: 9.40
7.19 – 10.16

4.60 – 6.51

Figure 4. Titration curve of egg white solution with 0.1 N NaOH and 0.1 N HCl
 12 – 12.02 10.20 – 11.99

6.62 – 7.1

3.94 – 4.55

3.61 – 3.94

Figure 4. Titration curve of egg white solution with 0.1 N NaOH and 0.1 N HCl
 ARGININE
pKa: ∼12.00
12 – 12.02
LYSINE
pKa: 9.8 - 10.80
10.20 – 11.99
EQUIVALENCE POINT
INITIAL PH: 9.40

HISTIDINE
PKA: 6.5 - 7.4
6.62 – 7.1

GLUTAMIC ACID
PKA: 4.1 - 4.8 {
ASPARTIC ACID
3.94 – 4.55
PKA: 3.90 - 4.80
3.61 – 3.94

Figure 4.1. Titration curve of egg white solution with 0.1 N NaOH and 0.1 N HCl
HENDERSON-HASSELBACH
CONCLUSION
COMPLEX PROTEINS
SUPPORT THE
BODY'S BUFFERING
SYSTEM
GROUP 1
 GROUP 1
BERSALONA, MARK

THANK YOU JENDRIX

CANSINO, CZARLYN GRACE

FOR DE CASTRO, CHARISSE

EBRADA, JOEVICK ANNE

LISTENING! GUILLEN, ALEXA IZABELLE

HERAMIA, AARON EZEKIEL
MANGIWET, GEILAN
MOLINA, CHARMAINE
RAMOS, KATHLYN MARY
 LITERATURE REFERENCES
Alsante, K. M., Friedmann, R. C., Hatajik, T. D., Lohr, L. L., Sharp, T. R., Snyder, K. D., & Szczesny, E. J. (2001).
Degradation and impurity analysis for pharmaceutical drug candidates. Handbook of

Modern Pharmaceutical Analysis, 85–172. https://doi.org/10.1016/s0149-6395(01)80006-4

Cotterill, O. J., Gardner, F. A., Cunningham, F. E., & Funk, E. M. (1958). Titration Curves and Turbidity of Whole
Egg White. Sciencedirect.com. https://www.sciencedirect.com/science/article/pii/S0032579119481595/pdf?
md5=9b0daa628c06eb8f62a41592732f5546&pid=1-s2.0-S0032579119481595-main.pdf

Lieberman, M., & Peet, A. (2015). Marks’ essentials of medical biochemistry: a clinical approach. Wolters Kluwer.

Meisenberg, G., & Simmons, W. H. (2017). Principles of medical biochemistry. Philadelphia, Pa Elsevier.

Pelley, J. W. (2007). Elsevier’s integrated biochemistry. Mosby.

Rodwell, V. W., Bender, D. A., Botham, K. M., Kennelly, P. J., & P Anthony Weil. (2018). Harper’s illustrated
biochemistry (31st ed.). Mcgraw-Hill Education.