GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY

INTRODUCTION
WIHTOUT ENOUGH G6PD TO PROTECT THE RBCs CAN BE DAMAGE OR DESTROYED AN INHERITED CONDITION IN WHICH THE BODY DOES NOT HAVE ENOUGH OF THE ENZYME GLUCOSE-6PHOSPHATE DEHYDROGENASE

WHAT IS G6PD??
PROTECTS RBC FROM POTENTIALLY HARMFUL BY PRODUCT THAT CAN ACUCUMULATE WHEN A PERSON TAKE CERTAIN MEDICATION OR WHEN THE BODY IS FIGHTING AN INFECTION

HELP RED BLOOD CELLS FUNCTION NORMALLY AND ONE OF THE ENZYMES THAT HELP THE BODY PROCESS CARBOHYDRATE AND TURN THEM INTO ENERGY

X-LINKED DISEASE WHICH AFFECTING MALES AND CARRIED BY FEMALES

SYMPTOMS
PALENESS DARK TEA COLOR URINE EXTREME TIREDNESS

ENLARGEMENT OF SPLEEN

RAPID HEART BEAT

JAUNDICE

RAPID BREATHING

LABORATORY INVESTIGATION
BLOOD COUNT AND FILM NORMAL * SHOWS RED CELL WITH ABSENT HEMOGLOBIN BITE AND BLISTER CELL AND POLYGROMASIA

HEINZ BODY MAY BE SEEN IN ARETICULOCYTE PREPARATION WITH SUPRAVITAL STAINING

HEMOLYSIS ALSO CAN BE SEEN

RETICULOCYTES HAVE HIGHER ENZYMEACTIVITY AND INCREASED IN RETICULOCYTE COUNT

SCREENING TEST
TO SCREEN FOR THE PRESENCE OF THE ENZYME GLUCOSE-6-PHOSPHATE DEHYDROGENASE BY UV LIGHT. G6PD + NADP + -------- GLUCUNATE-6-P NADPH + H+

TECHNIQUE
PUT A DROP OF BLOOD ON A CLEAN PAPER KEEP IT DRY

PUNCH THE PAPER WITH PUNCHER AND LET THE PIECES DROP INTO A TUBE OF G6PDH REAGENT

WAIT FOR 10 MINUTES AT ROOM TEMPERATURE

DROP 100L OF THE MIXTURE ON A CLEAN PAPER

READ UNDER FLOURESENCE LIGHT

TREATMENT
THE OFFENDING DRUG OR FAVA BEAN IS STOPPED ANY UNDERLYING INFECTION IS TREATED BLOOD TRANFUSION.

THANK YOU..