CRISPR

PRESENTED BY:
TAUSIF IQBAL
SP18-BSO-101
BBS-7B
 Introductio
n
• CRISPR/ Cas Stands for Clustered Regularly
Interspaced Short Palindromic Repeats

• This system was first discovered in 1987

• Named as CRISPR in 2002

• Gene Editing technology

 WHAT IS CRISPR/CAS--9 ?
• Family of DNA Sequences in Bacteria

• Palondromic Repeat followed by

spacer DNA

• It is a Prokaryotic immune system

• Cas-9 ( Crispr Associated Protein 9 )

Biological structure of Cas-9

• Cas-9 protein has six domains:

 REC 1

 REC-2

 Bridge Helix

 PAM interacting domain

 HNH domain

 RuvC domain
Common Mode of
Action
Types of Cas-9 Nucleases

There are 3 types of Cas-9 nucleases:

 Implications of CRISPR

• Genome wide Screening

• Genome Editing

• Transcriptional Regulation

• Repurposing dCas9

• Therapeutic applications

• Ecological applications
 Limitations of CRISPR

• T7 Endonucleases 1 mutation detection assay

• Incidence of off-target mutations?

• How far targeting efficiency in huamns?

• Potential to edit germline, will this be ethical ?