MOLECULAR

DIAGNOSTICS
RT-PCR
REVERSE TRANSCRIPTION - PCR
WHAT IS MOLECULAR DIAGNOSTICS??

• Molecular diagnostics is a collection of techniques used to analyze 

biological markers in the genome and proteome, and how their cells express their
genes as proteins, applying molecular biology to medical testing.
• In medicine the technique is used to diagnose and monitor disease, detect risk,
and decide which therapies will work best for individual patients,  and in 
agricultural biosecurity similarly to monitor crop- and livestock disease, estimate 
risk, and decide what quarantine measures must be taken.
REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION

• Reverse transcription polymerase chain reaction is one of many variants of polymerase chain reaction.
• It was introduced in 1977.
• The discovery of reverse transcription during the study of viral replication of genetic material led to
development of RT-PCR.
• RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA
transcripts from RNA.
• It allows identification of pathogenic organisms that are difficult to culture by detecting their genetic
material, RNA.
REFERENCE

• Reverse transcription polymerase chain reaction – Wikipedia

• Molecular diagnostics – Wikipedia
• https://www.thermofisher.com