CULTURE MEDIA

SYSTEMS
Classification of Culture media

• A. Consistency
• B. Composition
• C. Function and usage
Classification of culture media
A. Consistency:
I. __________: contains no solidifying agent.
(Ex. Broth, Infusion, Milk)
II. __________: contains __________agar
(Red algae/Gelidium)
III. __________:
Liquifiable: with __________agar
Non liquifiable: __________for anaerobe; __________ for fungi
Classification of culture media
B. Composition
I. __________: with known exact composition
II. ____________________: Contains at least 1 non-chemically
defined component like: blood, serum, yeast extract.
III. __________: recommended for organisms that cannot grow
in cell free media
Classification of culture media
B. Composition
III. Tissue:
a. Mccoy cell: from ____________________ b. Vero
cell: from ____________________
c. A549 cell: from ____________________
d. Hela cell: from ____________________
e. Hep-2 cell: from ____________________
Classification of culture media
C. Function and Use
i. General purpose medium
ii. Selective medium
iii. Enriched medium
iv. Enrichment medium
v. Selective/Differential medium
vi. Differential medium
Classification of culture media
C. Function and Use
i. General purpose medium
- Supports the growth of non-fastidious organism
- example: __________ agar/broth and __________ soy
agar/broth
Classification of culture media
C. Function and Use
ii. Selective medium
- Select the growth of particular organism and prevents
other organism via inhibitors
Ex. Lowenstein Jensen: ____________________
Thayer martin, Martis lewis, New York City for
____________________
Classification of culture media
C. Function and Use
iii. Enriched medium
- Contains nutrient supplements
Ex. Blood agar plate: contains 5% ____________________
(horse/sheep)
Chocolate agar
Classification of culture media
C. Function and Use
iv. Enrichment medium
- Can enhance the growth of microorganism
ex. Alkaline peptone water for ____________________
Selenite and tetrathionate broth for
______________________________
Classification of culture media
C. Function and Use
v. Selective/Differential medium
- allow certain types of organism to grow and differentiate
and inhibit the growth of other organism
ex. Mannitol salt agar: _____________________________
Thiosulfate citrate bile salt: ____________________
Salmonella-shigella agar
Eosin methylin blue
Classification of culture media
C. Function and Use
vi. Differential medium
- provide distinct colonial appearance of organisms to
know their identification

ex. Triple sugar iron; contains lactose, glucose and sucrose

ROUTINE PRIMARY MEDIA FOR AEROBE AND ANAEROBES

Blood agar plate

- Enriched medium that will grow most
non-fastidous bacteria
- Tryptic soy agar with 5% sheep blood
- Allows differentiation of hemolysis
ROUTINE PRIMARY MEDIA FOR AEROBE AND ANAEROBES

Blood agar plate

Types of hemolysis
. Alpha hemolysis
- Beta hemolysis
- gamma hemolysis or the
nonhemolytic
ROUTINE PRIMARY MEDIA FOR AEROBE AND
ANAEROBES

Alpha hemolysis
-__________around the
colony, it may be narrow or wide
-__________of RBC’s
ex. Streptococcus pnuemoniae,
viridans strep and some
enterococci
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
Beta hemolysis
-__________around the
colony, it may be narrow or wide
-__________Complete lysis of
RBC’s
ex. Group A,B strep and listeria
monocytogenes
ROUTINE PRIMARY
MEDIA FOR
AEROBE AND
ANAEROBES
Gamma hemolysis
-NO zone of hemolysis
-NO lysis of RBC’s
ex. Some enterococci
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
Target hemolysis
-Double zone hemolysis/Target
hemolysis around the colony, it may be
narrow or wide
ex.
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
Wide zone hemolysis
-aka the Alpha-prime(a’)
- small area of intact RBC around
colony surrounded by a wider zone
of __________
ROUTINE PRIMARY MEDIA FOR AEROBE AND
ANAEROBES

Chocolate Agar
- Enriched medium for haemophilus
and neisseria
- Tryptic soy agar with 5% sheep blood
- Upon RBC lysis it supplies
__________ factor which the
source of nutrient for the bacteria
ROUTINE PRIMARY MEDIA FOR AEROBE AND ANAEROBES

Columbia colistin nalidixic acid agar(CNA)

- Selective medium for gram positive
microorganisms
- colistin nalidixic acid suppresses the
gram negative bacteria
- Contains 5% sheep blood
ROUTINE PRIMARY MEDIA FOR AEROBE AND ANAEROBES

Phenyl ethyl alcohol agar ( PEA)

- Selective medium for gram + cocci and anaerobic
gram negative rods
- Phenyl alcohol inhibits __________
__________ bacteria
- Contains 5% sheep blood
ROUTINE PRIMARY MEDIA FOR AEROBE AND
ANAEROBES

Streptococcal selective agar (SSA)

-selective medium for ____________________

-contains ____________________
- Contains 5% sheep blood
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
Eosin methylene blue
- Selective medium for isolation of enteric
gram-negative rods
- Eosin methylene inhibits gram-positive
- Lactose fermenters are _______ or
_______ colony
- E. coli produces green metallic sheen
- Non-lactose fermenters are colorless colony
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
MacConkey agar (MAC)
-Selective, differential medium for isolation of
enteric gram-negative rods
-Bile salts and crystal violet inhibits most
gram-positives
- Lactose fermenters are __________

- Non-lactose fermenter: __________

ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

MacConkey sorbitol agar (SMAC)

- Selective media for ______________
- ______________ does not ferment sorbitol
therefore the colonies are ______________
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

Hektoen enteric agar (HE)

- Bile salts, bromothymolblue, and acid
fuschin inihibits normal GI flora
- Non pathogens are ______________ to
______________
- Non-lactose fermenter are
______________ to ______________
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

Xylene lysine deoxycholate (XLD)

-Selective, differential medium for
isolation of ______________ and
______________ in stool
-deoxycholate inhibits most gram
negative rods and gram positive
Have 4 types of colonies
 ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
Xylene lysine deoxycholate (XLD)
Have 4 types of colonies
Yellow: ______________
Yellow with black centers: ______________
Colorless or red colonies: ______________
Red colonies with black center: ______________
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

Salmonella-shigella agar(SS)
- Selective medium for Salmonella
and shigella
- Salmonella and shigella do not
ferment lactose therefore their
colony are colorless
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

Gram-negative broth
-Selective enrichment medium for
isolation of salmonella and shigella from
stool and rectal swabs
- Subculture onto selective/differential
agar after ______________ of
incubation
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

Selenite broth
- Enrichiment broth used for recovery of
salmonella from stool
-subculture to enteric media after
______________
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES
Tetrathionate broth
Enrichment broth for recovery of
salmonella from stool
Bile salts ad thiosulfates inhibits gram +
and enterobacteriaciae
Subculture after ______________ of
incubation
ROUTINE PRIMARY
MEDIA FOR AEROBE
AND ANAEROBES

Campylobacter blood agar

- Selective enrichment medium for isolation of
campylobacter from stool
- Incubates plates in Increased CO2 at 42°C