Bioactivity, in vitro antimicrobial properties

and chemical composition of selected

Bryophytes of Bangladesh

Sadeya Alam Dinaa*, Shaikh Bokhtear Uddin

and Mohammad Omar Faruquea
Ethnobotany and Pharmacognosy Lab
Department of Botany, University of Chittagong
Chattogram-4331
 WEL COME

Bioactivity, in vitro antimicrobial

properties and chemical composition of
selected Bryophytes of Bangladesh

Sadeya Alam Dinaa*, Shaikh Bokhtear Uddin

and Mohammad Omar Faruquea
Ethnobotany and Pharmacognosy Lab
Department of Botany, University of Chittagong
Chattogram-4331
Introduction

 Despite being the second biggest  Khan (1955) initiated research on

category of terrestrial plants, bryophytes' bryophytes and did an outstanding work. He
therapeutic potential remains unknown discovered some new species of Riccia
and unexplored
 From 1998 to 2006 Banu-Fattah studied on
 According to various published and the taxonomic studies on the Acrocarpous
unpublished documentation, bryophytes Mosses of Bangladesh of different family,
contain active components that are genus
extensively applied as antibacterial,
antifungal, cytotoxic and insecticidal  However, antimicrobial activities and others
agents bioassay of bryophytes is yet to be
investigated in Bangladesh

3
Aims and Objectives
Aims: to investigate the biological significance of bryophytes to treat
microbial diseases
Objectives
1 Taxonomic Study

2 Antimicrobial Study

3 Phytochemical Study

4 Pharmacological Study
Study Area

Location: Fatehpur Union 22° 28′ 11.64″ N, 

91° 47′ 25.44″ E
22 kilometres north of the Chittagong city

Area: 2110 acres of hilly areas

47species of mosses (bryopsida) was found in CU

campus

Dumortiera hirsuta, a liverwort was found by Syed

Hadiuzzaman in 1975 from University campus.
Methods

Collection
Documentation
of of Identification
ethnomedicinal uses plants

 Qualitative
Phytochemical Test  Quantitative

Antimicrobial Test Pharmacological Test

 Disc diffusion  Antioxidant
 Agar well diffusion  Anti-inflammatory
Preparation of
plant extracts In vitro tests

6
Ethnomedicinal uses

Marchantia sp. Pallavicinia sp. Riccia sp. Dumortiera sp.

Used to treat Used to treat Used to treat Used as

 Boils  Microbial infection  Ringworms  Antibiotic

 Jaundice  Fungal infection  Wound healing  Antileukemic
 Inflammation
Collection of plants
1 2
 Collected from Chittagong University campus at mature stage
 January to March found suitable for collecting Bryophytes
 7 species of Bryophytes were collected with their GPS location

Sl no. Names of Bryophytes GPS reading

3 4
1. Marchantia polymorpha N 22°27’3” E 91°47’5”

2. Marchantia papillata N 22.2234° E 091.5017°

3. Dumortiera hirsuta N 22.46521° E 091.77662°

4. Pallavicinia lyellii N 22.46538° E 091.77969° 6

5
5. Cyathodium sp. N 22.2816 ̊ E 091.4750 ̊

6. Anthoceros sp. N 22°27’29” E 91°47’12”

7. Riccia sorocarpa N 22.2740 ̊ E 091.47506 ̊

7
Anatomical Identification
 Collected from Chittagong University campus at mature stage
 January to March found suitable for collecting Bryophytes
 7 species of Bryophytes were collected with their GPS location

Sl no. Names of Bryophytes GPS reading

1. Marchantia polymorpha N 22°27’3” E 91°47’5”

2. Marchantia papillata N 22.2234° E 091.5017°

3. Dumortiera hirsuta N 22.46521° E 091.77662°

4. Pallavicinia lyellii N 22.46538° E 091.77969°

5. Cyathodium sp. N 22.2816 ̊ E 091.4750 ̊

6. Anthoceros sp. N 22°27’29” E 91°47’12”

7. Riccia sorocarpa N 22.2740 ̊ E 091.47506 ̊

 Preparation of plant extracts

1 2 3
Air dry after washing

Pasting with mortar and pastel

Soaking in methanol 4 5 6

Evaporating in water bath

Preserving at 4°C for further use

 Antimicrobial test

Disc Diffusion
Method
1. Anthoceros sp.
2. Dumortiera hirsuta Agar cup well Diffusion
3.Pallavicinia lyellii Method
4. Marchantia papillata 1. Pallavicinia lyellii
5. Cyathodium sp. 2. Marchantia papillata
6. Marchantia polymorpha
7. Riccia sorocarpa 3. Riccia sorocarpa
 Phytochemical Test Pharmacological Test
Qualitative test Quantitative Test
 Coumarins  Flavonoids
 Cardiac glycosides  Phenols Antioxidant test Anti-inflammatory test
 Glycosides  Tannin
 Flavonoids
 Phenol
 Quinone
 Steroids
 Saponins
 Tannins DPPH free Albumin
 Terpenoids
 Phytosterol radical denaturation
 Phlobatannin scavenging technique
activity
Results
Disc Diffusion Method

 Extract of Sample  E. coli V. cholerae B. cereus B. subtlis S. aureus

1. Anthoceros sp. 00 00 09 00 00
2. Dumortiera hirsuta 07 00 08 08 08
3.Pallavicinia lyellii 12 14 00 10 00
4. Marchantia papillata 08 10 08 09 10
5. Cyathodium sp. 07 00 08 08 00
6. Marchantia polymorpha 07 07 07 08 10
7. Riccia sorocarpa 08 09 08 07 11
8. Distilled water 00 00 00 00 00
 Disc Diffusion Method
14
14
12
12 11
10 10 10 10
10 9 9 9
888 8 8 88 8 8 8
Zone of nhibition (mm)

8 7 7 777 7

2
00 00 0 0 0 0 0
0

Disk diffusion method of selected bryophytes

against (A) E. coli, (B) V. cholerae, (C) B.
cereus, (D) B. subtilis, (E) S. aureus
E.coli V. cholerae B. cereus B. subtilis S. aureus
 Business Proposal

Agar cup well diffusion method 25

20 20
20 19
18
Dose 15 15 15
Name of the 15 14
13 13 13 13
Sl no. Name of the Plant 1212 12
bacteria 2.5mg 5mg
11
10 9

E. coli 13 19 5
1. Pallavicinia lyellii B. subtilis 11 13
0
V. cholerae 15 20 0

E. coli

S. aureus
E. coli
B. subtilis

V. cholerae

B. subtilis

V. cholerae

S. aureus

V. cholerae
B. subtilis - 9
2. Marchantia papillata V. cholera 15 18
S. aureus 14 20
E. coli 12 12 Pallaviciinia Marchantia Riccia
3. Riccia sorocarpa V. cholera 12 13 lyellii papillata sorocrpa
S. aureus 13 15 2.5mg 5mg
 Agar cup well diffusion method

P. lyellii against: (A and B) E. coli, (C and

D) B. subtilis, (E and F) V. cholerae

M. papillata against: (A and B) B. subtilis,

(C and D) V. cholerae, (E and F) S. aureus M. papillata against : (A and B) E. coli, (C
and D) V. cholerae, (E and F) S. aureus
 Qualitative Phytochemical Test
Name of the Phytochemicals
Sample Name Car. Phl- Phy-
Flv. Sap. Tan. Phe. Ter. Str. Gly. Qui. Cou.
gly. tan. ster.

P. lyellii + + + + + + + + + + - -

M. papillata + + + + + + + - + + - -

R. sorocarpa + + + + + + + - + + - -

Note: Cou.= Coumarin, Car.gly.= Cardiac glycosides, Flv.= Flavonones, Gly.= Glycosides, Phe.= Phenol, Sap.= Saponins, Str.=
Steriods, Tan.= Tannins, Ter.= Terpenoids and Qui.= Quinone, Phl-tan.= Phlobatannin, Phy-ster.= phytosterol
 Quantitative Test
Total Phenolic Compound Total Flavonoid Compound
Total phenolic content as Total flavonoid content as
Name of samples Name of samples
GAE (mg/g) QE (mg/g)
Pallavicinia lyellii 57.72 ± 1.86 Pallavicinia lyellii 31.27± 0.58
Marchantia papillata 29.98 ± 1.09 Marchantia papillata 46.59± 1.60
Riccia sorocarpa 14.34± 6.81 Riccia sorocarpa 33.76± 0.73

50

70 45
40
60
35
50
30

Total flavonoid
40
25
Total phenol

30
20
20 15
10 10
0 5
0
Pallavicinia Marchantia Riccia
lyellii papillata sorocarpa
Sample name
Sample names
 Total Tannin Compound
60

50

Total tannin content as

Name of samples 40
TAE (mg/g)

Total Tannin
30
Pallavicinia lyellii 50.29± 0.82
Marchantia papillata 39.10± 0.68 20

10
Riccia sorocarpa 8.38± 1.0
0
Pallavicinia lyellii Marchantia papillata Riccia sorocarpa

Name of the samples

 Conc.
(µg/ml)

200
400
% Scavenging activity of P. lyellii

56.05
76.49
% Scavenging activity of M. papillata

60.03
69.25
Antioxidant test

% Scavenging activity of R. sorocarpa

47.73
66.90
% Scavenging activity of Std. ascorbic acid

92.46
96.86 P. lyellii

M. papillata
IC50

R. sorocarpa

Ascorbic Acid
120
120

100
100
300
252.95
80
80 250
200.06
185.25
200
60
60

150
40
40
100

50 23.60
20
20

0
00
P.L MP RS STD
00 50
50 100
100 150
150 200
200 250
250 300
300 350
350 400
400 450
450

P.L
P.L Linear
Linear (P.L)
(P.L) MP
MP
Linear
Linear (MP)
(MP) RS
RS Linear
Linear (RS)
(RS)
STD
STD Linear
Linear (STD)
(STD)
 Conc.
(µg/ml)
% of albumin denaturation of P. lyellii

% of albumin denaturation of M. papillata

% of albumin denaturation of R. sorocarpa

% of albumin denaturation of ASA

Anti-inflammatory Test

P. lyellii

M. papillata
IC50

R. sorocarpa

ASA
 Anti-inflammatory Test

100
90
80
70
60
50
40
30
20
10 250 218.01
00 50 100 150 200 250 300 350 200 178.99

P.L Linear (P.L) 132.29

150
M.P Linear (M.P)
R.S Linear (R.S) 100
63.06
Std Linear (Std)
50

0
P.L M.P R.S Std
 Conclusion
 the majority of the extracts have the capacity to inhibit bacterial
growth
 presence of various phytochemicals through qualitative and
quantitative test
 have the capacity to inhibit free radicals

 have the capacity to inhibit protein denaturation

Selected species could be the potential source for the development of

traditional treatment system of Bangladesh and to develop antimicrobial
agents against the microbial diseases
THANK YOU