Cordyceps Militaris mushroom

Cordyceps Militaris : Medicinal Mushroom

Cordyceps militaris is a potential harbour
of bio-metabolites for herbal drugs and
evidences are available about its
applications for revitalization of various
systems of the body from ancient times.

Amongst all the species, C. militaris is

considered as the oldest source of some
useful chemical constituents.

People most commonly use Cordyceps

for athletic performance, kidney
disorders, liver problems, and sexual
problems.

www.biobritte.co.in
 C. Militaris Functions
• Pro-sexual, • Anti-fibrotic,
• Anti-inflammatory, • Steroidogenic,
• Anti-oxidant/Anti-aging, • Hypoglacaemic,
• Anti-tumour/Anti-cancer/Anti-leukemic, • Hypolipidaemic,
• Anti-proliferative, • Anti-angiogenetic,
• Anti-metastatic, • Anti-diabetic,
• Immunomodulatory, • Anti-hiv,
• Anti-microbial, • Anti-malarial,
• Anti-bacterial, • Anti-fatigue,
• Anti-viral, • Neuroprotective,
• Anti-fungal, • Liver-protective,
• Anti-protozoal, • Reno-protective
• Insecticidal, • Pneumo-protective
• Larvicidal,

www.biobritte.co.in
 Constituents
• The constituents of medicinal mushroom Cordyceps militaris, especially the anti-
cancer agent cordycepin (3′-deoxyadenosine), are expected to play evolutionary
roles in the pharmacognosy sector in future.

www.biobritte.co.in
• Cordyceps militaris mushroom may be used to support energy levels, endurance,
and healthy lung capacity.
• Its most prized compound, the energy-giving cordycepin, is molecularly similar to
adenosine, which exists in all human cells.
• Adenosine is used by the body to regulate heart rate and balance metabolic energy.
• Since our bodies don't differentiate between Cordycepin and adenosine,
Cordycepin is essentially free energy!

www.biobritte.co.in
Cordyceps Benefits
www.biobritte.co.in
 USES & EFFECTIVENESS
May Boost Exercise Performance
• Cordyceps are thought to increase the body’s production of the molecule
adenosine triphosphate (ATP), which is essential for delivering energy to the
muscles.
• This may improve the way your body uses oxygen, especially during exercise.

Anti-Aging Properties
• Researchers believe their antioxidant content may explain their anti-aging
potential.

Potential Anti-Tumor Effects

• Test-tube and animal studies suggest Cordyceps may have the potential to treat
cancer, as well as some side effects of cancer treatments. However, these effects
have not been shown in humans, and more research is needed.

www.biobritte.co.in
 USES & EFFECTIVENESS
May Help Manage Type 2 Diabetes
• Cordyceps contain a special type of sugar that may help treat diabetes.

Possible Benefits for Heart Health

• Cordyceps may benefit heart health by helping prevent arrhythmias and lowering
levels of triglycerides and “bad” LDL cholesterol.

May Help Fight Inflammation

• Cordyceps is said to help fight inflammation in the body.

Can be employed in libido-enhancing applications

• Cordyceps offers a natural and powerful alternative to libido-enhancing
pharmaceutical drugs, proven in many studies and used by various cultures
worldwide to treat male impotence, improve erectile function, sperm production
and stamina, and increase sexual desire in both men and women.

www.biobritte.co.in
 Setup
• First room
General work-autoclave-media preparation
• Second room
Laminar air flow and inoculation
• Third room
Mushroom growing room
• Fourth Room
Harvesting drying and other work
Layout
 Basic requirements

AUTOCLAVE LAMINAR ORBITAL

STERILIZATION AIR SHAKER
FLOW
ASEPTIC WORK
SPAWN/SEED
20 K-100 K
30-40 K 30 K-60 K
WEIGHING BALANCE AC

2-3 K 30-36 K
 Other cost

• One AC Room insulation • Miscellanous

• Chemicals • Blades, scalpel
• Glasswares- • Forcep
conical flask • Chairs
Beaker • Stools
Test tube • Jars
Glass rod
• Racks, Table platforms
 Min cost to start
Capacity 5-8 kg
• 4-5 Lakhs
• 3 lakhs instrument
• 60 k chemicals
• 40 k jars
• 40 k racks
• 20 k electrical fitting
• 40 k other

• To increase capacity increase ac room

Cordyceps Rooms
 First room
General work-autoclave-media preparation
 Second room
Laminar air flow and inoculation
 Third room
Mushroom growing room
Cordyceps laboratory
Incubation stage
Growing stage
Growing stage
 Cultivation Procedure
Procedure
• Obtain mother culture or isolate.
• Liquid seed preparation
• Substrate media preparation
• Incubation
• Harvesting and Drying
• Marketing
Obtain mother culture or isolate.
Chemical name:

Potato Dextrose Agar (Himedia)

Company: Himedia

1. Weigh dehydrated powder

add into distilled water
autoclave.
2. Fill in tube
3. Solidify and cool

4. Ready to use
Obtaining Sub-culture
 Liquid seed preparation
Glucose 40 g/L
Tryptone 10 g/L
Yeast Extract 6 g/L
MgSO4·7H2O 0.5 g/L
Magnesium Sulphate
K2HPO4·3 H2O 0.5 g/L
Potassium Phosphate
KH2PO4 0.5 g/L

Potassium Di-Hydrogen

Phosphate
Substrate media preparation
Shaking and Incubation
Orbital Shaker
 Cordyceps Jar Preparation
• Components For 25 Jars

• Brown rice + Chemical liquid base

 LIQUID MEDIA for 25 Jars
For 500 Ml liquid Base Chemical Volume

Distilled Water 500 ml

Glucose 20 g

Peptone 7g

Yeast 3g

Mgso4.7H20 0.5 g
Magnesium Sulphate
EGG 1

VIT. B CAPSULE 1
1.Add 30 gm grain substrate to bottle.
2.Add liquid base-50 ml
Autoclave

STORE ON RACKS AT 22 C
 Stages

10-25 DAYS
26-40 DAYS 40-55 DAYS
Cordyceps products