Lab Techniques

By
Dr Muhammad Tariq Aziz
November 04,2022
1
Nov 04, 2022
FECAL EXAMINATION TECHNIQUES
 Fecal Examination Techniques
Faecal Examination

Qualitative method
Quantitative Method

Stoll’s Dilution Method

Un-Concentration Mc Master Method
Concentration
method method

Direct method

Simple sedimentation
Floatation method Centrifugal sedimentation
Formalin-ether concentration technique
ARTIFACTS
DIRECT METHOD
 Faecal Examination Techniques

Air bubble

Oocyst of
Protozoa

Egg of Parasite

Cyst of Balantidium
coli
 QUANTITATIVE EXAMINATION OF FAECES

• Egg Counting Methods:

• For accurate information regarding to severity of infection egg count methods are
very useful by estimating eggs per gram (EPG) of faeces. 
 QUANTITATIVE EXAMINATION OF FAECES

• Egg Counting Method

• The McMaster technique is used for demonstrating and counting helminth eggs in faecal samples.
• Two beakers or plastic containers
• Balance
• Tea strainer, cheesecloth or dental napkin
• Measuring cylinder
• Stirring device (fork, spatula, tongue depressor)
• Pasteur pipettes and rubber teats
• Flotation fluid (choice of solution dependant on species expected to be present and availability of reagents)
• McMaster counting chamber
• Compound microscope
SOLUTION
• Eggs per gram (EPG) counts in different animals Vs levels of infection: -

Animal level of infection

Parasite
Mild Moderate Severe

Nematodes Cattle 50-300 300-600 >600

Sheep 100-500 500-2000 2000-6000
Horse 500 800-1000 1500-2000
Fasciola cattle 10-100 100-200 >200
spp.
Sheep 50-300 300-600 >600
SLIDE PREPARATION
SLIDE
PROTOZOA
?