NOTRE DAME OF MIDSAYAP COLLEGE

Group Report
Meet the Group

Haron Caven Clark

Salazar Cabaya
What is a
ELECTROPHORESIS?
• Electrophoresis is the separation of charged
compounds based on their electrical charge.

• The process of separating the charged constituents of

a sample by means of an electrical current.
ELECTROPHORESIS
AMPHOTHERIC
- A substance that can have a negative, zero or
positive charge depending on conditions. Anode (+)
It is a positively charge
ANION electrode
⚬- Negatively charge Cathode (-)
⚬- Migrates to the anode It is a negatively charge
electrode
CATION
⚬- Positively charge
⚬- Migrates to the cathode
ELECTROPHORESIS
• The mobility of the particle is affected by the following
factors:

- Net charge of the particle.

- Size and shape of the particle.
- Strength of the electric field.
- Chemical and physical properties of the medium.
- Electrophoretic temperature
 COMPONENTS OF AN
ELECTROPHORESIS

• POWER SUPPLY
• BUFFER
• SUPPORT MEDIUM
• SAMPLE
 POWER SUPPLY
• Supplies constant current or voltage in the
system.
• This drives the molecule through the support
medium.
• Drive force
• Voltage depends on the ionic strength of the
buffer.
 BUFFER

• Used to provide ions that carry a current and to maintain the pH

at a relatively constant value

• Ions that will enable the movements of the current

and the migration of the particles
 BUFFER
-pH and ionic strength of the buffer affects the
analyte.
-A mixture of proton-donating and proton accepting substances that
functions to maintain pH constant.

• Barbital (veronal) pH 8.6

• Tris-boric EDTA pH 8.7
 BUFFER
pH
- pH acidic=binds H+=ampholyte becomes positively charge,
cation=migrates to the cathode
- pH basic=loses H+=ampholyte becomes negatively charge,
anion=migrates to the anode

Ionic strentgh
LOW I.S = more charge will be carried=faster
mobility.
HIGH I.S-less charge will be carried-slower
mobility.
 SUPPORT MEDIA
• A network of interacting fibers or a polymer that is solid
but traps large amount of solvent in pores or channel
inside.

• Must not interact with the analyte.

Cellulose acetate

Agarose Gel

Polyacrylamide Gel
 SUPPORT MEDIA

CELLULOSE ACETATE
- Cellulose acetate is an insoluble cellulose derivative
regarded as a nontoxic, nonirritant, and biodegradable
material.
- It is heat-resistant and less hygroscopic
- Separates serum proteins into 5 bands
 SUPPORT MEDIA

AGAROSE GEL
- Used as a purified fraction of agar.
- It is neutral and, thus, does not produce
electroendosmosis.
- Separates proteins into 10-15 bands.
 SUPPORT MEDIA

POLYACRYLAMIDE GEL

- Separation of protein base on charge and molecular

size.
- Separates serum proteins into 20 or more bands.
SAMPLE
Thank you!