CHEMICAL PATHOLOGY

Professor Idania Hidalgo

Topic:
1. Introduction to chemical pathology, role of chemical pathology in health-care,
application of biochemical techniques, measurements in chemical pathology,
1.1. concentration of a substance,
1.2. concentration of some analytes,
1.3. methods used in the chemical pathology laboratory,
1.4. basic statistics, precision and accuracy in biochemical tests,
1.5. meaning of normal and reference ranges,
1.6. specimen handling in chemical pathology,
1.7. contamination separation of red cells from serum/plasma,
1.8. reference ranges of analytes which are frequently encountered and therefore useful
to memorize and result
Objectives: -To demonstrate the importance of chemical clinical service
-To describe the different test used in this area
-To discuss application of biochemical techniques, measurements in chemical
pathology
-To define normal referent ranges

Reference: Laboratory test Handbook 3th edition

The reference range problem and biological variation Pag 11-15
 INTRODUCTION
• Chemical pathology (also known as
clinical biochemistry , clinical chemistry ,
medical biochemistry or pure blood
chemistry ) is the area of pathology that
is generally concerned with analysis of
body fluids .
• The physician, after examining the patient, decides and orders a test, and
collects and transports the patient’s samples; this constitutes the pre-
analytical limb of the cycle of events.
• In the analytical limb the sample is received
by the laboratory and analyzed.
 Group 1

1 NKIZIBWEKI ATURINDA BMS/0284/143/DU

2 ABDULRAHMAN MUKEERE 2021-04-03829

3 FRANCIS KATUNGI 2020-08-00182

4 INNOCENT BWAMBALE 2020-08-01812

5 KAALE SHARON 2019-08-07612

6 WAJAMBUKA FAISAL 2020-08-00012

7 EDVEGU CHRISTOPHER 2020-08-00025

8 HASALYAERINAH 2020-08-00053

9 JOHNSON THEMBO 2020-08-00063

10 EMMA LWANGA MUGABE 2020-08-00072

11 ARIHOONA FARUKU 2020-08-00103

12 LUWUGGE CATHERINE 2020-08-00111

13 MUKISA CHRISTOPHER 2020-08-00120

14 MUGABE ANDREW 2020-08-00124

15 JOY ROBERT AARON 2020-08-00129

 What are the methods used in
Chemical Pathology Laboratory?

LUWUGGE CATHERINE 2020-08-00111

 Standard operating procedures
• The preparation of test procedures comes
under the broad heading of Standard
Operating Procedures (SOPs).
• SOP is a clear, concise and comprehensive
written instruction of a method or procedure
which has been agreed upon and authorized
as the operating policy of the department
• In general, SOPs, which mainly contain
detailed descriptions of each analytical
method, are essential for maintaining the
same analytical quality over a long period of
time. The procedures are a prerequisite to
correct transfer of methods from one
laboratory to another
• The post-analytical limb consists of the transfer of the
result to the physician and a meaningful interpretation
of the laboratory data by the physician, followed by
necessary action.
What is the role of Chemical Pathology in
health care?

KAALE SHARON 2019-08-07612

Common chemical pathology tests
• Common chemical pathology tests include:
Electrolytes Sodium ; Potassium ; Chloride ;
Bicarbonate.
• Renal (Kidney) Function Tests Creatinine ;
Blood urea nitrogen ; Urate
• Liver Function Tests Total protein ; Albumin ;
Bilirubin ; direct; indirect; total AST ; ALT ;
GGT; ALP ;
• Cardiac Markers Troponin Myoglobin CK-MB
ANP (A type Natriuretic peptide)
• Minerals Calcium ; Phosphate ; Magnesium ;
• Blood Disorders Iron ; Transferrin ; Vitamin
B12 Folic acid
• Lipids profiles Cholesterol TG HDL-
cholesterol VLDL-cholesterol LDL-cholesterol
• Hormones Testosterone Prolactin
Progesterone Estrogen LH FSH TSH T3,T4
• Globulins ; Glucose ; C-reactive protein ;
Glycated hemoglobin (HbA1c). Arterial
blood gases Stone analysis(Renal and Gall
stones)
Homework:
1. Precision and accuracy in biochemical tests

2. Sensitivity and specificity in laboratory tests

3. The reference range problem and biological

variation

4. Factors which can influence the value of an

analyte

Laboratory test Handbook 3th edition

 Group 2

16 DANNY PETER 2020-08-00187

17 KISAAKYE ANGELLA 2020-08-00253

18 NOAH DERICK MUWANGUZI 2020-08-00294

19 ATYANG TOBBY 2020-01-00306

20 EDGAR BALABA 2020-08-00318

21 MARVIN RUKUNDO 2020-08-00324

22 JOSHUA KITUMA 2020-08-00333

23 ANDREW KAGGWA SSERUJJA 2020-08-00355

24 TIMOTHY ODONG 2020-08-00371

25 MATSIKO SAMUEL 2020-08-00386

26 CHARLES BULAFU 2020-01-00609

27 MAWULA TONNY 2020-08-01129

28 TUT KONY NYANG HANNAH 2020-08-01147

29 NOKRACH DESIRE ODONG 2020-08-01166

30 ASIINA NANSOVE 2020-08-01260

 Group 3

31 NAJJUMA BETTY 2020-08-01417

32 SENYONGA STEPHEN 2020-08-01557

33 OKOT JAMES BOSS 2020-08-01751

34 NAKISOZI MEBLE GLORIA 2020-08-01854

35 JUSTICE SSENTEZA 2020-08-01873

36 KOOLBERT NSIIMIRE 2020-08-02014

37 LOUSE RENNY 2021-01-02068

38 NDIZIHIWE ALEX 2020-08-02297

39 ADIO REEM SALAH HASSAN 2021-04-03303

40 KIMBUGWE RONALD 2021-04-03378

41 MUNGI BELINDA MARY 2021-04-03460

42 SHARMARKE KHADAR 2021-04-03522

43 PATRICK OKOT OCHOLA 2021-04-03523

44 KAPCHEWERE DOMINIC 2021-04-03639

45 KISOMBO MICHAEL PETER W 2021-04-03644

 group 4

46 ENGULU PHILLIPO 2021-04-03761

47 MBAMBU ANITA 2021-04-03779

48 NAMUYANJAH ANNAH VERONICA 2021-04-03800

49 IBWALA JOSEPH OSIA 2021-04-03806

50 ABAHO ROBERT 2021-04-03827

51 NAHWERAJONNAH 2021-04-03881

52 NAKANDI JOAN 2021-04-03888

53 DERICK BENJAMIN AMACHA 2021-04-03921

54 TUMUKUNDE MARK AARON OINE 2021-04-03925

55 KALANZI ELTON BENON 2021-04-04020

56 MOHAMED FATMA KHAMIS 2021-04-04658

57 MUNGA HARRISON MVUKO 2021-08-04898

58 SILAS PETER RWOT OMIO 2021-08-04908

59 OJELAH FAVOUR OGOH 2021-08-04916

60 MORRIS MUCHANGI KARIMI 2021-08-04919

 Group 5

61 PETER NYANGO MAGRETH 2021-08-04928

62 NTABANA RUTH RUZINDANA 2021-08-04983

63 KAFIRITI MERCY 2021-08-05021

64 JOSHUA RAINAH NAMAGALI 2021-08-05107

65 LUCKY SHEEBAH AINEBYONA 2021-08-05114

66 INNOCENT LAMWAKA 2021-08-05124

67 ANNET MARY NAKALO 2021-08-05128

68 WALTER RUTOMA 2021-08-05132

69 ANTONIO RAMIREZ BERNIS SAMUEL 2021-08-05134

70 JOEL VIANNY BWIRE 2021-08-05136

71 JOVAN SSEMOMBWE 2021-08-05138

72 JOSHUA WODEYA 2021-08-05157

73 MOHAMED ALI ABDIRIZAK 2021-08-05163

74 MARIAN MABLE TUHIRIRWE 2021-08-05168

75 SIRIVE NSADHA 2021-08-05181

 Group 6

76 FRED NSUBUGA 2021-08-05222

77 ELAM SSEBADDUKA 2021-08-05225

78 DASANI GWAIVU 2021-08-05232

79 PEACE ARABA 2021-08-05270

80 MORGAN DERIS KOLOKOLO 2021-08-05271

81 MARIA NATASHA NAMUJJA 2021-08-05272

82 KABUGU DAVID TENDO 2021-08-05273

83 ISABELLA ILLUGA 2021-08-05285

84 JACOB OWEKA 2021-08-05333

85 LUCAS WAYODI 2021-08-05378

86 SAMALIE NAKITTO 2021-08-05403

87 ADAM ZARIA 2021-08-05404

88 HENRY MUKULA 2021-08-05423

89 MARIAM S NAKATUDDE 2021-08-05425

90 MARY SHEILAH KEMBABAZI 2021-08-05448

 Group 7

91 WYCLIFF MUMBERE 2021-08-05477

92 JULIANA STELLA KARUNGI 2021-08-05487

93 EMMANUEL WANDERA 2021-08-05490

94 STEPHEN KENYI BESHIR 2021-08-05502

95 STEPHEN NINSIIMA 2021-08-05521

96 ISSACK GABOW IKRAN 2021-08-05589

97 ANITAH AKANKWASA 2021-08-05600

98 MUHONJA VERONICA 2021-08-05788

99 FAUSTO SANTUS APUUN 2021-08-05880

100 JOSHUA TRUST BWAMBALE 2021-08-05893

101 CRANIMER AHUMUZA 2021-08-05996

102 AARON TAYEBWA 2021-08-06098

103 RAYMOND OKELLO 2021-08-06294

104 HOPE NALUGO KYOMUHENDO 2021-08-06303

105 EPHRAIM OTYEKHO MWANJE 2021-08-06313

 Group 8

106 MICHELLE KYOMUHENDO 2021-08-06361

107 EMMA OMIRIO 2021-08-06372

108 ROBERTA NAMAKULA KATENDE 2021-08-06485

109 SHAKIRAH NALUTAAYA 2021-08-06491

110 JOSHUA WAKHOOLI 2021-08-06504

111 GERALD ARIHO 2021-08-06533

112 YOFESI MUHINDO 2021-08-06540

113 DAPHINE NALUGO 2021-08-06599

114 SIMON MUHUMUZA 2021-08-06604

115 ALVIN TAYEBWA 2021-08-06630

116 TWINEMUGABE AUGUSTUS 2021-08-06632

117 ISAAC NATUMANYA 2021-08-06634

118 HENRY MUKIIBI 2021-08-06641

119 WAHABU KASIRYE 2021-08-06652

120 GLORIA NANKABIRWA 2021-08-06653

 Group 9

121 HENRY BWAMBALE 2021-08-06660

122 ESTHER ATUGONZA 2021-08-06663

123 VIVIAN TUKASHABA 2021-08-06666

124 JOSEPH KASULE 2021-08-06681

125 LAWRENCE SEBUUMA 2021-08-06709

126 NELSON NAKASALA 2021-08-06712

127 SHEILA AHWERA 2021-08-06765

128 JOSHUA SSEMAMBO 2021-08-06766

129 DAVID ELVIS SSEBALAMU 2021-08-06769

130 VIOLA NASSAKA 2021-08-06773

131 ABDU KHARIM SSEKANDI 2021-08-06776

132 RONAH NAMANYA 2021-08-06777

133 JOHN NSAMBA SSEMBAJJWE 2021-08-06814

134 FRED ANZO 2021-08-06817

135 CRIFUBICK BAVAMUNO 2021-08-06823

136 JOSELINE ALINAITWE 2021-08-06827

 Group 10

137 CONCEPTA AHAISIBWE 2021-08-06829

138 MATIA ALINITWE 2021-08-06841

139 MARVIN OKELLO EKWANG 2021-08-06853

140 ANDREW KIHUMURO 2021-08-06856

141 GADDAFFI MUHANGUZI 2021-08-06861

142 SHALLON TWINOMUJUNI 2021-08-06862

143 ANGEL DEBORAH KYENKYA 2021-08-06867

144 ASIMIRE WINNIE 2021-08-06872

145 REAGAN SSENGO 2021-08-06879

146 STEVEN LUBEGA 2021-08-06880

147 DENIS KAJUMBI 2021-08-06886

148 SOLOMON AKUGIZIBWE 2021-08-06890

149 MENYA SAMUEL MATOVU 2021-08-06895

150 BENJAMIN NIWAGABA 2021-08-06902

Group 1, 2,3,4,5 Friday 21/07/2023 time 8:00 am to 10:am

1. Chemistry Lab

2. Microbiology Lab

3. Parasitology lab

4. Histopathology lab

5. Haematology Lab
Group 6,7,8,9,10 Friday 21/07/2023 time 2:00 pm to 4:00 pm

6. Chemistry Lab

7. Microbiology Lab

8. Parasitology Lab

9. Histopathology Lab

10. Haematology Lab

CHEMICAL PATHOLOGY PRACTICAL CLASS 1

21/07/2023

Topic: Classification of Chemical Pathology Service.

Acquisition of Chemical Pathology data.

The use of Chemical Pathology Service.

Collection of blood specimen.

Manual and automated techniques.

Objectives: To indicate correct use and managing of chemical pathology service

To show information to accompany requests for laboratory test

To educate in the code and conduct for laboratory personnel
To instruct the collection of blood specimen
To present the Manual and automated techniques.
1) Prepare specimen collection material2) Instruct patient appropriately 3) Collect, preserve and
transport specimen carefully 4) Separate serum or plasma properly 5) Maintain proper record of collection 6)
Handle the specimen carefully

 Reference: District Laboratory Practice in tropical countries; second edition part 1, Monica
cheesbrough Henry's Clinical Diagnosis and Management by Laboratory Methods, 21st ed.
CHEMICAL PATHOLOGY PRACTICAL CLASS 2

21-07 -2023

Topic: Quantitative and qualitative test

The international system of unit

Reference ranges

Pre-analytical, analytical and post analytical stages

 
Objectives: To show Quantitative and qualitative test
To indicate correct use of the international system of unit
To instruct the Reference ranges
To present Pre-analytical, analytical and post analytical stages
Reference: District Laboratory Practice in tropical countries; second edition part 1, Monica cheesbrough
Henry's Clinical Diagnosis and Management by Laboratory Methods, 21st ed.
Tietz test book of clinical chemistry and molecular diagnostic