Ecotoxicology and Environmental Safety

Impact factor – 7.129

Simran Deep Kaur(03716001319)
 OBJECTIVE

Topics to INTRODUCTION

be OVERVIEW OF METHODOLOGIES

covered RESULTS AND DISCUSSION

CONCLUSION
 OBJECTIVE

To develop a two-step oily

sludge bioremediation
process

The first step was designed

to remove most of the TPH The second step of
in the oily sludge by using biodegradation of the
oil recovery method with remaining TPH with SCBA.
biosurfactant.
 INTRODUCTION
• Large amounts of petroleum hydrocarbons are released into the environment during the process
of oil exploitation, storage, transportation ,oily sewage treatment ,that has consequently caused
significant environmental pollution problems.

5–86 wt% of total petroleum hydrocarbon (TPH)

Oily sludge accounts Heavy metals include nickel, chromium, zinc, lead,
for:- manganese and copper

Toxic substances, such as benzene series, phenols,

anthracene, and pyrene,

• The bridge structures between various organic pollutants and inorganic solids in oily sludge enhance their stability.

• There are a range of oily sludge treatment approaches, including physical, chemical ,and biological methods
 Limitations associated with Advantages associated with
chemical and physical methods :- biological methods:-
 Use of large amount of chemical solvents  Low cost

 Harsh operating conditions  Environment-friendly

 High energy consumption  Mild operating conditions

 Simple operation

By selecting specific microorganisms it has demonstrated strong ability to degrade the TPH and organic matters in oily
sludge, which can eventually be transformed into harmless materials e.g. CO2 and H2O.

A variety of microorganisms have been reported capable of using petroleum hydrocarbons as the sole carbon source
and energy, such as Pseudomonas, Achromobacter, Candida digboiensis, Micromonospora, Bacillus subtilis, and
Leuconostoc mesenteroides .
 METHODOLOGIES
• Bacterial isolation and screening
The strains Luteimonas huabeiensis sp. Nov ,Chelatococcus daeguensis, Pseudomonas aeruginosa and
Bacillus subtilis were isolated from oily sludge in Xinjiang Oilfield
• Shake cultivation

• Preparation of solid complex bacterial agent (SCBA)

• Preparation of biosurfactant solution

• Determination of bacterial density in oily sludge

• Processing oily sludge with SCBA and indigenous microorganisms

• Processing oily sludge with combined two-step treatment

• Large-scale field tests and Chemical analysis
TPH removal = (oil content before treatment - oil content after
treatment)/(oil content before treatment) × 100%.

• High-throughput sequencing
RESULTS AND DISCUSSION
Characterization
of oily sludge
Growth of different strains in different temperature
and oily sludge leaching solution
Biodegradation with different bacteria
Combined two-step process for bioremediation of
oily sludge with high TPH content
 Effects of solid agents dosage and composition on
biodegradation of oily sludge

Effects of solid agents dosage and composition on biodegradation (Conditions: Temperature, 30 ◦C;
processing time, 6 weeks; water content, 25%. Bioagent addition (wt%), A: 1.0% SCBA, B: 2.5%
SCBA, C: 5.0% SCBA, D: 5.0% SCBA + 5.0% wood chips, E: 5.0% SCBA + 10.0% wood chips).
Large-scale field tests
 • Four screened strains showed high
efficiency for the degradation of total
petroleum hydrocarbons (TPH) and the
reduction of COD of the oily sludge, at
96.6% and 92.6% respectively.
• The mixed strains together with bran
prepared in form of SCBA exhibited
CONCLUSION improved performance compared to
individual strains.
• The use of SCBA provided advantages
over commonly used liquid media for
storage and transportation.
• The large-scale field test confirmed the
feasibility and superiority of the
technology for industrial applications.