ECWA COLLEGE OF HEALTH TECHNOLOGY KAGORO,

KADUNA STATE

DEPARTMENT OF MEDICAL LABORATORY SCIENCE

SUMMARY OF PRACTICAL EXPERIENCE
FROM
DATE: JANUARY 16th- MAY 12th 2023
@
UNIVERSITY OF ABUJA TEACHING HOSPITAL (UATH)
COMPILED
BY
FRANCIS THANKGOD
MLT/2021/0895
MEDICAL MICROBIOLOGY

 This is a branch of medical

laboratory science that deals
with the study of micro-
organism.
BACTERIOLOGY

 It a branch of medical
laboratory science that deals
with the study of bacteria
SOME TEST CARRIED OUT IN BACTERIOLOGY
LABORATORY

 Film making
 Gram staining
 Plate pouring
 Culture and media preparation
 Catalase test
 Sterilization
 Ziehlnelseen
 Urine examination
SPECIMEN USED IN BACTERIOLOGY

 Urine
 Stool
 Aseptic fluid
 ECS
 Blood
 Sputum
 Swap(hvs, wound)
SOME MATERIALS USED IN
BACTERIOLOGY LABORATORY
 Microscope
 Autoclave
 Centrifuge
 Petri dish
 Incubator
 Wire loop
 Weighing balance
 Sensitivity disc
 Forceps
 TITTLE:GRAM’S STAINING
 AIM: to differentiate between grams positive bacteria and grams
negative bacteria
 METHOD: grams staining technique
 MATERIALS: microscope, slide, crystal violent, lugol’s iodine,
acetone, neutral red, Bunsen burner, wire loop, staining rack, culture
plate, cotton wool, pipette,
 PRINCIPLE: the acidic component of a cell have affinity to basic
dye and the basic component of the cell have affinity to acidic dye.
SMEAR MAKING WITH SOLID
CULTURE
 A drop of normal saline was placed at the center of
a clean grease, free, slide,
 A wire loop was sterilized and allow to cool
 A colony of the sample was picked and emulsified
in the drop of normal saline evenly
 It was allowed to air dry
 PROCEDURE
 The dried smear was pass over a Bunsen
burner flame three times
 It was allowed to cool
 It was placed on a staining rack
 It was covered with crystal violent for 60sec
 It was rinsed with clean water
 It was covered with lugols iodine for 60sec
 It was rinsed with clean water
 It was decolourized with acetone briefly
 It was rinsed with clean water
 It was counter stained with neutral red for 120sec
 It was rinsed with clean water
 The back of the stained smear was clean with dry cotton
wool
 It was allowed to air dry
 It was examined microscopically using x100 objective
lens
RESULT

 Gram’s positive cocci seen (blue or

violet).
CHALLENGES
 The following are the
 At the collection bench , I was unable to collect blood
from most of the infants effectively
 Most test I feel I can carry out, but I was not allowed to
do.
 Only few staff were ready to assist us in term of teaching
on bench
 I was told that most of the work are for scientist and not
technicians.
RECOMMENDATION

 I strongly recommend our school authority to

maintain the posting of our students to the
facilities because the hospital is well
equipped, there is orderliness of work,
experienced scientists and technicians and the
work load is much.
CONCLUSSION

 Despite the challenges we faced, I was able to conclude

my practical posting. And through the help of God, I was
able to learn a lot from the various department and I can
carry out some investigations in those various department.
I therefore wish to thank the unit staffs and their head,
HODs, admin, who made sure that we didn’t go back the
way we came.