Bioassays: Monoclonal

Antibodies as an Example.

Characterization is key in the production of biotherapeutics. Learn more about

monoclonal antibodies and the methods used to analyze them.

by Shubham Jarande
Introduction to Bioassays
What are bioassays? Why is characterization important?

Bioassays are a type of biological experiment that Accurate characterization ensures that the
use living cells or organisms to test the effects of biotherapeutic will have the desired effect on the
a compound. body.
Monoclonal Antibodies:
Definition and Manufacturing

Monoclonal antibodies are identical immune system proteins made in a

laboratory. They are created by fusing mouse spleen cells with myeloma cells,
and then selecting the resulting hybridoma cells which produce the desired
antibody.
Monoclonal Antibodies in Bioassays
Uses: Antibody-based bioassays are widely used to detect
the presence of specific markers or quantify
compounds.

Advantages: Monoclonal antibodies are highly specific, potent,

and stable, making them more reliable than other
detection methods.

Examples: Some common applications of monoclonal

antibodies in bioassays include drug development,
clinical diagnosis, and disease research.
Methods of Characterization
1 Analytical Ultracentrifugation

Used to measure a molecule's size, shape, and density.

2 Circular Dichroism Spectroscopy

Used to determine the secondary structure or protein conformation of a molecule.

3 Isothermal Titration Calorimetry

Used to measure the strength or affinity of the interaction between two molecules.
Application of Monoclonal Antibodies

Research and Development Clinical Diagnosis and Treatment

Monoclonal antibodies play an important role in the They are used to diagnose and treat diseases such as
development of new drugs and therapies. cancer, autoimmune disorders, and infectious diseases.
Conclusion
Bioassays are important tools in drug development and characterization, and
monoclonal antibodies are a valuable component in this process. Their
specificity, stability, and potency make them a key player in many bioassays,
from clinical diagnosis to research and development.