Florescence (forster) Resonance energy

transfer (FRET)
• Technique to study molecular interaction
within the living cells
• Applications
Protein-protein interactions
Ligand binding to receptor
Molecular dimerization
Activation of G proteins
 Requirements For FRET
• Two proteins tagged with different florophors
• Interacting molecules must be > 10nm appart
• Overlap of emission wavelength of one
flourophore with excitaion wavelenght of the
other flourophor
•
Principle – FRET