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S TAINING OF B ACTERIA

M icrobiology
BSN Semester: I
Unit:9
IFTIKHAR UL HAQ
DEMONSTRATOR
INS-KMU, PESHAWAR
UNIT OBJECTIVES

Perform the procedure of staining bacteria

Execute the staining of bacteria with the proper professional


standards
Stain & Staining
Types of Staining
Staining Methods
Gram Staining
Gram Staining
Organizing the staining bottles
Principle of gram staining
Why Gram +ve bacteria resist decolourization by
alcohol/acetone mixture but the gram –ve ones can not?
Gram Staining Procedure
Gram Staining Procedure results
Errors during Staining

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