ELFA & ELISA

TEST
 ELISA
 ELISA (enzyme-linked Immunosorbent Assay) adalah jenis
immunoassay enzim fase padat yang digunakan dalam deteksi
protein spesifik dalam sampel biologis dengan bantuan
pengembangan warna melalui reaksi enzimatik.

 DIRECT
 INDIRECT
 SANDWICH
 COMPETITIVE
 DIRECT ELISA

1.Antigen is immobilized onto the wells of a 96-well polystyrene plate via passive adsorption.
2.An enzyme-labeled primary antibody (e.g. HRP-labeled primary antibody) specific for the target antigen is added to the wells and directly
binds to the antigen.
3.A respective enzyme substrate (e.g. TMB (11012) a suitable substrate for HRP) is added, which upon reaction with the enzyme, produces
a visible colorimetric output that can be measured by a spectrophotometer or absorbance microplate reader.
INDIRECT ELISA
SANDWICH
 ELFA
 ELFA test stands for enzyme-linked fluorescent assay. It is
a new version of enzyme-linked immunosorbent assay
(ELISA). It is used for the detection of antibiotics against
chlamydia trachomatis. It also helps in measuring
antibodies, antigens, proteins, and glycoproteins in
biological samples.
 The main difference between ELISA and ELFA is that, in ELISA, color development is the
detection criteria for the positive samples but, in ELFA, emitting fluorescence is the detection
criteria.

 ELISA and ELFA are two immunological methods used in the detection of proteins in
biological samples especially, antibodies and antigens. Although ELISA is a sensitive method,
ELFA is more sensitive than ELISA.
 Conclusion
 ELISA is a type of immunological assay which detects proteins in a
biological sample with the binding of specific antibodies. Here, the
detection method is the development of color through an enzymatic
reaction. On the other hand, ELFA is another type of immunoassay
which detects the presence of a specific protein in the sample with
the development of fluorescence through an enzymatic reaction.
Therefore, the main difference between ELISA and ELFA is the type
of substrate in the enzymatic reaction.