BASICS OF LABORATORY

SAFETY

By
Inayah Fitri, M. Si
Before Beginning Any
Laboratory Exercise
• Know the dangers
associated with all
materials you will use in
this laboratory exercise
• Know the proper way of
using all the materials
provided for the exercise
• Know all the protective
measures you must use
during the exercise
Personal Protection
• Laboratory coat
• Wear an apron or lab coat in the proper
manner
• Alat ini untuk mencegah terjadinya
kontaminasi atau menghindari bahaya yang
terjadi akibat percikan zat-zat kimia yang
berbahaya.
• Gloves
• Wear gloves in the proper manner. Remove your gloves before
using instruments, telephone, and leaving the laboratory.
• Daya tahan sarung tangan terhadap bahan kimia tergantung
pada bahan sarung tangan (misalnya: karet alam; karet
neoprene; karet nitrile; dll.), mutunya dan ketebalannya.
• Untuk melindungi tangan dari bahan-bahan yang sangat panas
dianjurkan memakai "insulated glove" yang dibuat dari bahan
sintetis.
• Pelindung Mata dan Muka
• “Safety glases with side shield”
• “Face shield” = pelindung muka
• Wear a face shield in the proper manner.
• Kran pencuci mata = Eyewash fountain
• Safety shower
• Alat pernapasan = Respirator/Masker
Melindungi dari debu-debu, serat yang kecil
yang berbahaya atau dan uap atau gas yang
beracun.
• Hair
• Tie long hair neatly at
the back of the neck.
• Keep long or fluffy hair
tied up and out of the
way.
• Hair can contaminate
and be contaminated
by microbial cultures.
• Shoes
• Always wear shoes in
laboratory.
• Wear shoes that
enclose your entire
feet.
• Pemadam Kebakaran = Fire Extinguishers
• Ada beberapa jenis pemadam kebakaran, seperti Air
(water extinguisher), tepung (dry powder
extinguisher), C02 (Carbon dioxide extinguisher),
Halon, Busa, pasir, dll
Emergency Procedures

• Know where all exit doors

are located
• If a reagent splashes onto
your face or eyes, wash
your face at the eyewash
station for several
minutes
Emergency Procedures

• If an irritating or harmful
reagent splashes onto
your body, use the
emergency shower to
wash your body for
several minutes
• Know where the fire
extinguisher and fire
blanket are located
Emergency Procedures
• If a reagent spills onto the workbench or floor,
notify the instructor immediately for cleanup
instructions.
• If a glass container gets broken, notify the
instructor immediately for cleanup
instructions.
• Report all accidents, injuries and close calls
immediately to the instructor.
Good Laboratory Conduct
• Follow all instructions
• Do not play in the
laboratory
• Do not use any laboratory
equipment until you are
told to do so
• Do not perform any
laboratory procedure
until you are told to do so
Good Laboratory Conduct
• Do not bring food,
beverages or tobacco
products into the
laboratory
• Do not apply cosmetics in
the laboratory
• Do not eat or drink in the
laboratory
• Restrain loose clothing
and jewelry
Good Laboratory Conduct
• Do not practical jokes
• Do not play in
laboratory
• Do not rough house
 Before You Leave The
Laboratory
• Clean your workbench with
disinfectant at the beginning
and at the end of the
laboratory exercise
• Leave all equipment, samples
and reagents in the lab
• Leave your lab coat (apron) in
the lab
• Wash your hands with soap
and water
 Laboratory requirements

• Laboratory coat
• Matches or lighter
• Soap
• Waterproof permanent marker
• Small or medium size towel
• Sealable plastic bag
• Rubber band
• General Microbiology Manual
 Acquaint yourself with microbiology lab

Microscope Dettol

Staining solutions Microscopic slides

Bunsen burner
 Forceps

Sterile cups Wooden tongue depressor and swabs

Normal saline
Syringes
 Incubator
Autoclave
Laminar Air Flow
 Culture Plates or Petri
Dishes

Flaming Nichrome loop

Plastic Loops and Needles

Anaerobic Jar Candle jar
Anaerobic station
 Enterotube

API 20 E
Liquid media in tubes Solid media in tubes
Different forms of slants
Blood culture bottles
Antibiotic disks
 Di bawah ini tanda-tanda yang sering digunakan
secara internasional:

POISON : Bahan-bahan yang bersifat racun

Flammable :Bahan yang mudah terbakar
Corrosive: bahan yang dapat merusak jaringan hidup
Irritant: Sedikt saja masuk ke tubuh dapat membakar
kulit, selaput lendir atau sistem pernapasan
Toxic : Sedikit saja masuk ke tubuh dapat
menyebabkan kematian atau sakit keras
Oxidising Agent: Bahan yang dapat menghasilkan
panas bila bersentuhan dengan bahan lain terutama
bahan-bahan yang mudah terbakar
Explosive: Bahan yang mudah meledak bila kena
panas, api atau sensitif terhadap gesekan atau
goncangan
Radioactive: Bahan-bahan yang bersifat radioaktif
High voltage: Peringatan tegangan tinggi
No smooking: Area dilarang merokok
No fire: Area dilarang menyalakan api