A synopsis presentation on

FORMULATION DEVELOPMENT,
CONTAINING POLYHERBAL EXTRACT AND THEIR
EVALUATION FOR ANTIFUNGAL ACTIVITY

In partial fulfilment of the requirement for the degree of

Research Proposal
For Registration of Ph.D. Program
By
SHUBHAM SHARMA
Enrolment No. 20221429
Under the guidance of
Dr. Sunil Gupta

Mangalayatan University, Aligarh, Uttar Pradesh December, 2023

 Contents
 Introduction

 Literature Review

 Objective of the study

 Scope of the study

 Methodology

 Gantt chart

 Reference
 Introduction
 Fungal diseases are caused by a wide variety of fungi that are commonly found in the
environment.
 Invasive fungal infections rarely occur in healthy people, but fungi can cause serious infections
in individuals with weakened immune systems.
 Fungi live outdoors in soil and on plants and trees as well as indoor surfaces and human skin.
 There are millions of different species of fungi, but only a fraction of those is known to make
people sick.
 Anyone can get a fungal infection, such as athlete’s foot or a toenail infection, but people with
weakened immune systems are more likely to get serious fungal infections.
 Fungal infection of human body's skin is common dermatological problems throughout the
world.

 It has been observed that many peoples suffer from fungal infections.

 Fungal infections occurring superficially on the nails, hairs & skin and are difficult to treat.

 Among one of the most common causes of tinea are dermatophytes.

 Some other widespread superficial cutaneous fungal infections are Tinea Versicolor or
Pityriasis Versicolor and candida infections.
Herbal Medicine:
Traditional medicine is described as follows by the World Health Organisation (WHO):

"The health practices, approaches, knowledge and beliefs incorporating plant based medicines

and exercises, applied singularly or in combination to treat, diagnose and prevent illnesses or

maintain well-being.“

In India, drugs of herbal origin have been used in traditional systems of medicines such as

Ayurveda, Unani and siddha medicines since ancient times.

Among these systems, Ayurveda is most practiced and widely accepted alternative system of

medicine in India.
Poly-herbal Formulations:

Poly herbal formulations are the formulations containing two or more than two herbs are called
poly herbal formulations (PHF).

The popularity of poly herbal formulation is due to its high effectiveness towards a number of
diseases.

Drug formulation in Ayurveda is based on two principles: Use as a single drug and use of more
than one drug, in which the latter is known as PHF.

This key traditional therapeutic herbal strategy exploits the combining of several medicinal
herbs to achieve extra therapeutic effectiveness, usually known as poly-pharmacy or herbalism.
 Literature review
 VarunS. Mali et. al., (2013) was PREPARED AND EVALUATE THE ANTIFUNGAL PROPERTY
OF A POLYHERBAL FORMULATION CONTAINING ACHYRANTHES ASPERA AND
PLECTRANTHUS AMBOENICUS.In the present study, an attempt was made to investigate a detailed
Pharmacognostic, physicochemical and antifungal activity of leaf extracts of Plectranthus amboenicus and
Achyranthes aspera.

 Km. Shiva et. al., (2021) they are FORMULATE AND EVALUATE OF TOPICAL ANTIFUNGAL
GEL OF FLUCONAZOLE USING ALOE VERA GEL.The results indicate that the studies of
formulation and evaluation for topical anti-fungal gel of fluconazole using aloe Vera gel has potential for
avoid side effect like liver damage, kidney damage and safe in pregnancy. It was calculated that
formulation F1 was best formulation as compare other formulation. The basic gel definitely succeeding
and the proficient development and production of excellence pharmaceutical gel depends on their base
knowledge of physio-chemical properties and stability.
 Rajasekaran Aiyalu et. al., (2021) was Formulate and evaluate the topical herbal gel for the
treatment of arthritis in animal mode. Anti-arthritic activity of the developed topical herbal gel
formulation may be due to the presence of luteolin and apigenin in methanol leaf extracts of
Cardiospermum halicacabum and Vitex negundo.

 Ajinkya Mate et. al., (2021) was formulate and evaluate the Poly-herbal cream for the Management
of Acne. Recently herbal medicines are more considered as safe with fewer side effects than synthetic
drug for the treatment of acne vulgaris. Therefore, In the global market. Natural remedies including
herbal formulation are in great demand. It is a very good attempt to formulate and evaluate the polyherbal
anti-acne cream along with the stability studies.

 R. Bhramaramba et. al., (2015) was developed the Formulation and evaluate the Gel Containing
Terminalia chebula Retz., Leaves Extract.The plant Terminalia chebula Retz., was selected for the
study, whose extract was very useful in the treatment of wounds. Literature survey revealed that this plant
is used traditionally for various ailments, especially for its wound healing property
 Objectives of the Research Work
 The object of the present study is to develop poly herbal formulation for treatment of fungal

infection thereby bypassing the side effects caused by various synthetic drugs.

 Conduct in vitro studies to assess the anti-fungal activity of the formulated product against

common fungal strains.

 To assess compatibility of poly herbs in poly herbal formulation.

 To assess stability profile of poly herbal formulation.

 To assess safety profile of poly herbal formulation in vitro.

 Scope of the study
 To evaluate the various physical parameters.
 To determine the effect of treatment on the fungal culture.

 Select specific herbs with known anti-fungal properties for the formulation.

 Conduct phytochemical analysis to identify active compounds responsible for anti-fungal effects.

 Investigate compatibility and synergistic effects among chosen herbs.

 Optimize the formulation for enhanced stability, bioavailability, and efficacy.

 Experiment with various extraction methods to obtain the maximum active compounds from the
herbs by using Bioactivity guided fractionation method.
 Methodology
The planned work will be executed in accordance with the following strategy in an effort
to meet the stated goals: The study is planned in eleven stages as given below:
1. Literature Survey:
2. Collection and Authentication of Plant Materials;
3. Pre-formulation Study:
1. Pharmacognostic study:
 Morphological character:
 Colour
 Odour
 Taste
 Shape
 Size
 2. Physicochemical evaluation of leaves of each plant:

 Ash value
 Total Ash value.
 Water Soluble Ash value
 Acid Soluble Ash value
 Sulphated ash value

 Extractive value

 LOD

4. Extraction of Plant crude drug:

 Soxhlet Extraction
5. Physicochemical evaluation of extracts:

 Color

 Odor

 Nature,

 Test for Alkaloids

 Test for Glycosides

 Test for Flavonoids

 Test for Tannins

 Test for saponins

 Test for Terpenoids

 Test for steroids

6. Incompatibility study:

 Fourier transform infrared spectroscopy (FTIR)

7. Preparation of polyherbal Formulation:

8. Evaluation of Polyherbal formulation:

 Color, Homogeneity, Determination of pH, Appearance

 Extrudability study and Spreadability study

 Assay of the drug

 Antifungal activity

 PET, Impurity

10. In-vitro drug release studies:

11. Stability study:

 Gantt Chart
Task Name JULY 2023 JAN 2024 JULY 2024 JAN 2025 JULY 2025

Literature Review
Procurement of plant material
and authentication, selection of
excipients and Pre-formulation
study
Extraction of plant material,
physicochemical evaluation of
extract, Incompatibility study
and preparation of polyherbal
formulation

Evaluation of polyherbal
formulation and invitro drug
release study

Stability Study

Publication and thesis writing

 Reference
1. Suva MA, Patel AM, Sharma N. A Brief Review on Acne Vulgaris: Pathogenesis, Diagnosis and Treatment. Res Rev J
Pharmacol 2016;4(1):1–12.

2. Prabu SL, Umamaheswari A, Kumar CA, Banumuthupriya M, Dhanasekaran D. Formulation and Evaluation of
Polyherbal Gel Containing Natural Antimicrobials for the Management of Acne Vulgaris. Int Res J Pharm
2017;8(5):65–69.

3. Prasad SB. Acne vulgaris: A review on pathophysiology and treatment. Asian J Pharm Clin Res 2016;9(4):54–59.

4. Sinha P, Srivastava S, Mishra N, Yadav NP. New Perspectives on Antiacne Plant Drugs: Contribution to Modern
Therapeutics. Biomed Res Int 2014;2014: 301304.

5. Mishra BP. A quest of Anti-acne Potential of Herbal Medicines for extermination of MDR Staphylococcus aureus. Int J
Pharm Sci Invent 2014;3(6):12–17.