Monday 11 March 2024

DNA Replication
STARTER:

1. What part of the DNA molecule doesz

this show?

2. What bonds hold the two strands of

DNA together?

3. Analysis of a sample of DNA found

that 39% of the nucleotides contained
thymine. Determine the percentage of
guanine in the same sample of DNA.
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 DNA Replication
• A semi-conservative process.

• Ensures genetic continuity.

• A specific enzymatic process.z

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 DNA Polymerase
• Only synthesises strands in 5’  3’ direction.

z
Describe the process of DNA replication
(5 marks) (QWC).

z
Describe the process of DNA replication (5 marks) (QWC).

z
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 Meselson and Stahl Experiment
• Proved semi-conservative replication.

• Used:

o E. coli bacteria
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o Growth mediums containing different

nitrogen isotopes (14N and 15N)

o Centrifugation
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z
Describe the experiment carried out by Meselson and Stahl
that demonstrates the semiconservative replication of
DNA.

Include an outline of the method used and a description of

the results obtained. You can use diagrams to help you. (5
marks)
z
Describe the experiment carried out by Meselson and Stahl that demonstrates the
semiconservative replication of DNA.

Include an outline of the method used and a description of the results obtained. You can use
diagrams to help you. (5 marks)