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Neurotransmission
(Membrane Potential)
3
Electrochemical Equilibrium
• For any given concentration gradient
[Ion]out – [Ion]in across a cell
membrane, there is a membrane
potential difference (i.e., electrical
gradient) that exactly opposes ion
movement down the concentration
gradient.
• To calculate the equilibrium potential for any concentration gradient, we use the
Nernst equation
• The Nernst equation is used for a cell that is freely permeable to only one ion
at a time.
• If the membrane is not permeable to a particular ion, that ion does not
affect the membrane potential.
• For mammalian cells, we assume that Na+, K+, and Cl- are the three ions
that influence membrane potential in resting cells.
The GHK Equation
P is the relative permeability of the membrane to the ion
• The GHK equation for cells that are permeable to Na+, K+,
and Cl-
• Cell has a resting membrane potential of -70 mV.
• Most cells are about 40 times more permeable to K than to
Na.
• As a result, a cell resting membrane potential is closer to the
EK of -90 mV than to the ENa of +60 mV.
• Na+ and K+ are leaked promptly and pumped back by the
Na–K ATPase that helps maintain the electrical gradient.
Parts of Neuron
• If the membrane suddenly increases its Na+ Membrane potential
permeability, Na+ enters the cell, moving
down its electrochemical gradient
• The wave of depolarization that moves through the cell is known as local
current flow.
Graded Potentials
• The strength of the initial
depolarization in a graded
potential is determined by
how much charge enters the
cell
1. Current leak:
The membrane of the neuron cell body has open leak channels that allow
positive charge to leak out
into the extracellular fluid.
2. Cytoplasmic resistance:
The cytoplasm provides resistance to the flow of electricity
The combination of the current leak and cytoplasmic resistance means that
the strength of the signal inside the cell decreases over distance.
Graded Potentials
• The trigger zone is the integrating center of the neuron and
contains a high concentration of voltage-gated Na+ channels in
its membrane.
• Na+ then flows into the cell, down its concentration gradient, and is
attracted by the negative membrane potential inside the cell.
Rising Phase of the Action Potential
• the K+ channels open, the membrane potential of the cell has reached +30
mV
• As K+ moves out of the cell, the membrane potential rapidly becomes more
negative, creating the falling phase of the action potential
Falling Phase of the Action Potential
• K+ continues to leave the cell through both voltage-gated and K+ leak
channels, and the membrane hyperpolarizes, approaching the EK of −90
mV. This after-hyperpolarization
• Retention of K+ and leak of Na+ into the axon bring the membrane potential
back to −70 mV
The influx (movement into the cell) of Na+ depolarizes the cell
K+ efflux (movement out of the cell), which restores the cell to the resting
membrane potential.
The Action Potential
Axonal Na+ Channels Have Two Gates
• How the voltage-gated Na+ channels could close at the peak of the
action potential when the cell was depolarized?
• starts the positive feedback loop: depolarization opens Na+ channels→ Na+ enters →
causing more depolarization and opening more Na+ channels in the adjacent membrane
Absolute and Relative Refractory Period
• A second action potential cannot be triggered for about 1–2 msec, a second
action potential cannot occur before the first has finished.
• This delay, called the absolute refractory period, represents the time
required for the Na+ channel gates to reset to their resting positions
• Action potentials moving from the trigger zone to the axon terminal cannot
overlap and cannot travel backward.
• The action potential that reaches the end of an axon is identical to the action potential that
started at the trigger zone.
• The depolarization of a section of axon causes positive current to spread through the
cytoplasm in all directions by local current flow
• The local current flow in the cytoplasm diminishes over distance as energy dissipates.
• Forward current flow down the axon would eventually die out were it not for voltage-
gated channels.
• The larger the diameter of the axon or the more leak-resistant the membrane, the
faster an action potential will move.
• The unmyelinated axon has low resistance to current leak because the entire axon
membrane is in contact with the extracellular fluid and it has ion channels through
which current can leak.
• Myelinated axons limit the amount of membrane in contact with the extracellular fluid.
Action Potentials Faster
Conduction of action potentials
• The myelin sheath creates a high-resistance wall preventing ion flow from the
cytoplasm.
• Action potential passes through alternating regions of myelinated axon and nodes of
Ranvier
• Each node has a high concentration of voltage-gated Na+ channels, which open with
depolarization and allow Na+ into the axon.
• Sodium ions entering a node reinforce the depolarization and restore the amplitude of
the action potential
• The apparent jump of the action potential from node to node is called saltatory
conduction
Conduction of action potentials
• In unmyelinated axons, channels must open sequentially all the way down the axon
membrane to maintain the amplitude of the action potential.
• In myelinated axons only the nodes need Na+ channels because of the insulating
properties of the myelin membrane.
• As the action potential passes along myelinated segments, conduction is not slowed by
channel opening.
• In myelinated axons:
only the nodes need Na+ channels because of the insulating properties of
the myelin membrane.
As the action potential passes along myelinated segments, conduction is
not slowed by channel opening.
Chemical Factors Alter Electrical Activity
• The concentration of K+ in the blood and interstitial fluid is the major determinant of the resting
potential of all cells
• This condition shows up as muscle weakness because the neurons that control skeletal muscles
are not firing normally.