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    Esin Coşkuner
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    A focal adhesion site, prior to failure, applies 37 nN of force onto The MEMS sensor. The device is quite simple and they can measure the magnitude of the adhesion force this device doesnPt have much control on the contact of the cell and the beam can only measure the force form on contact region Dec. 5 Strategy A strategy to independently manipulate mechanical compliance and surface chemistry: for small deflections, the deflection is directly proportional to the force applied by the attached cell.

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    A focal adhesion site, prior to failure, applies 37 nN of force onto The MEMS sensor. The device is quite simple and they can measure the magnitude of the adhesion force this device doesnPt have much control on the contact of the cell and the beam can only measure the force form on contact region Dec. 5 Strategy A strategy to independently manipulate mechanical compliance and surface chemistry: for small deflections, the deflection is directly proportional to the force applied by the attached cell.

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    13 views24 pages

    Cell Adhesion

    Uploaded by

    Esin Coşkuner
    A focal adhesion site, prior to failure, applies 37 nN of force onto The MEMS sensor. The device is quite simple and they can measure the magnitude of the adhesion force this device doesnPt have much control on the contact of the cell and the beam can only measure the force form on contact region Dec. 5 Strategy A strategy to independently manipulate mechanical compliance and surface chemistry: for small deflections, the deflection is directly proportional to the force applied by the attached cell.

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    Download as PPT, PDF, TXT or read online from Scribd
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    of 24

    A STUDY ON CELL ADHESION BY USING MEMS TECHNOLOGY

    Rui Qiao Xiaohui Tan


    ME-381 Dec. 5

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Presentation Outline
    Introduction and motivation Current MEMS systems in this area Our idea Conclusion

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Meaning of this research


    Understand the evolution of tissues and organs Manipulate the adhesion

    http://health.allrefer.com/ ME-381 Dec. 5 Xiaohui Tan, Rui Qiao

    Why MEMS?
    Cells and MEMS are in the similar scale Cells can survive on artificial surfaces The microfabrication technique is relatively mature

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Some of Current Models


    Single MEMS sensor

    A bed of microneedles

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Model I
    In-situ adhesion studies of a single living bovine endothelial cell using MEMS sensor
    The MEMS sensor consists of a cantilever beam made of Single Crystal Silicon (SCS), 1200 m long x 11.5 m deep x 4 m wide, coated by a thin native silicon dioxide layer.

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Experimental System
    1 m Resolution Liquid Media Tissue Culture Dish MEMS Cantilever X-Y-Z Stage

    PiezoActuator 20 nm Resolution

    1 m Resolution

    Endothelial Cell

    Microscope Objective

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Experimental Result
    The adhesion properties of a single living bovine endothelial cell was studied. Quantitatively it has been found that a focal adhesion site, prior to failure, applies 37 nN of force onto the MEMS sensor.

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Focal adhesion
    Procession of a breaking filopodia

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Discussion
    This work is one of the earliest studies on cell adhesion. The device is quite simple and they can measure the magnitude of the adhesion force This device doesnt have much control on the contact of the cell and the beam Can only measure the force form on contact region

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Model II
    Cells lying on a bed of microneedles: An approach to isolate mechanical force

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Strategy
    A strategy to independently manipulate mechanical compliance and surface chemistry: for small deflections, the deflection is directly proportional to the force applied by the attached cell

    3EI F ! 3 H L

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Fabrication

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Fabrication Contd

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Result

    Both the magnitude and direction of the adhesion forces can be obtained

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Advantages
    Less assumptions Can capture the magnitude also the direction of the force at the same time The mechanical property of the substrate can be easily changed without change the material of the substrate

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Disadvantage
    The resolution of this model only in micrometer scale while the focal adhesion is also in this scale Improving the resolution means increasing the density of posts in the array, which leads to a high requirement of deflection measuring precision

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Our idea
    Use the membranes to measure the focal adhesion forces. As the deformation is vertical, the dots could be very close without any interactions
    F
    Fr 4 16 (d 7  v (d !  4 2 Eh 3 1  v h 3  v h 1
    3

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    MEMS System
    Fabricate an array of membranes on a silicon wafer. Those membranes will deform under cell adhesion
    Cell

    Membrane

    Membrane

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Fabrication process
    Photolithograph Cast PDMS Electrical etching Microcontact printing
    wafer1 wafer2
    hydrophobic alkanethiol

    protein-resistant hydrophilic alkanethiol

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Measurement
    Use optical readout to detect the displacement of each membrane

    Beam splitter Photo Detector


    ME-381 Dec. 5

    Laser Beam

    Xiaohui Tan, Rui Qiao

    Discussion
    Advantage Can improve the spatial resolution Can determine the adhesion force from each focal contact site. Disadvantage Cannot get the direction of the force Readout device is complicated

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    Conclusion
    Introduced two MEMS system already used. Provided a new device, which needs experiment to prove With the size of device decreasing, NEMS system will act important roles in this area

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

    ACKNOWLEDGEMENT

    Professor Horacio D. Espinosa TA: Yong Zhu Team members

    ME-381

    Dec. 5

    Xiaohui Tan, Rui Qiao

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