LEMIGAS is the government research & technology institution operating in the area of upstream and downstream oil and

gas. This institution was founded in June 1965 as the proof of concern and willingness of Indonesian people to be able to manage the natural resources effectively. LEMIGAS located at Jalan Raya Cipulir Kav. 109, Cipulir, Kebayoran Lama, South Jakarta. Industrial Practice Work implemented at the Technology Research Group of Process, Group of Biotechnology, Laboratory of Bioprocess in November, 1st 2010 until January, 28th 2011 Increasing the capacity and strengthen student skills in preparation in accordance with analytical chemistry courses. Apply theory learned in school with a practice in laboratories where the implementation in Industrial Practices Work. Cultivate and strengthen students' professional attitude in order to enter employment. Verification is a performance test of standard method. Verification is done on a standard method before it is applied in the laboratory. Verification of a method intended to prove that laboratory capable to performing the test of the method with valid results. In the verification method, the performance to be tested is the test accuracy (t) and precision (F). T test comparing the average value from a row of data from experiments with actual average value which is the value that has been known from previous studies F test is used to indicate if there are significant differences between two methods based on calculation of standard deviation of each method. If the t or F value acquired is smaller than the value of t or F on the table, so difference between actual value, and value measurement result is not significant. That means these two values is said to be the same and valid. Chemical Oxygen Demand (COD) is the amount of oxygen (mg O2) needed to oxidize organic substances contained in 1 liter of water samples, with oxidizing K2Cr2O7 used as a source of oxygen (oxidizing agent). CaHbOc + Cr2O7 2- + 8 H+ CO2 + 2 Cr3+ + 4 H2O Cr2O72- + 6 Fe2+ + 14 H+ 6 Fe3+ + 2 Cr3+ + 7 H2O This 3 methods are same way; based on the determination of remaining oxidant left (K2Cr2O7). Organic substances present in the samples in boiling acidic conditions which can be oxidized with excess measured K2Cr2O7. In ASTM and EPA, the remaining K2Cr2O7, titrated using the indicator Ferroin. Color changes that occur from greenish yellow to red brown. And in Photometri SQ 118, into the solution passed monochromatic beam. Light energy that passes through the solution can only be adsorbed by dichromate. The amount of energy adsorbed by the dichromate is directly proportional to the concentration of dichromate in solution.

Salinity is the level of salinity or the percentage of salt dissolved in water. In oceanography, salinity not usually stated in percent but in parts per thousand (parts per thousand) or per mil (), approximately equal to the number of grams of salt for each kilogram of solution. AgNO3 + Cl AgCl (white) 2AgNO3 + CrO4 Ag2CrO4 (brownish red) Salinity using the argentometric method, this method uses titrant Silver Nitrate (AgNO3) 0.28 N and indicators K2CrO4. Will form a white precipitate AgCl until all chloride ions react. The presence of chromate ions is able to form a red brown precipitate. All of AgCl will precipitate first, because the solubility (KSP) is smaller than Ksp Ag2CrO4. Ion selective electrode work with the basic principle of the galvanic cell. With the measure the electrical potential generated by the membrane on a particular ion (for example Cl-) and compared with the reference electrode. Strength of the resulting potential difference is proportional to the concentration of the measured ions (selective). Dissolved Oxygen is the number of milligrams of oxygen dissolved in 1 liter of water (mg/L). MnSO4 + 2 KOH Mn(OH)2 (white) + K2SO4 Mn(OH)2 + O2 MnO2 (brown) + H2O MnO2 + 2 KI + 2 H2O Mn(OH)2 + I2 + 2 KOH I2 + 2 Na2S2O3 2 NaI + Na2S4O6 Oxygen in the sample will oxidizes MnSO4 that is added to solution in the alkaline condition that resulted MnO2 precipitate. With the addition of concentrated sulfuric acid and alkali-iodide-azide, it will be release iodine which is equivalent to dissolved oxygen. The freed iodine is then analyzed by iodometric titration method is by using thiosulfate and starch indicator to the colorless endpoint.
Accuracy Test (t Test ) of COD and DO that value of t smaller than t table. So, concluded that the difference between actual value and the value of measurement results acquired are not significant, meaning both are the same value. Precision Test (F Test ), which conducted on the COD method, Salinity based on the calculation of standard deviation of each method, found that the calculated F value is smaller from the F table, it was concluded that both methods were not significantly different. This means that all three methods have been verified.

In the analysis of COD, especially in samples containing high Cl- content, such as sea water, it is advisable to use the EPA 410.3 and also measured the concentration of Cl- in the sample first. The use of correction factors in each of the volume of equipment is one way for the analysis conducted to be more thorough. This is because the Cl concentration above 854 mg / L will affect the original concentration of COD caused by the reduction reaction of potassium dichromate into Potassium Chromate by Cl ions. Having found the concentration of Cl, then put in the formula effect chloride. COD concentration in order to obtain additional estimates by the Cl ion. Accuracy of chemical analysis is required, especially in the process of quantitative analysis. Routinely calibrating tool is also very important for the results found become more accurate and valid.

41 H2SO4 + 10 K2Cr2O7 + 2 KC8H4O4 --> 10 Cr2(SO4)3 + 11 K2SO4 + 16 CO2 + 46 H2O Since 10 mol of potassium dichromate has the same oxidation power as 15 mol of oxygen, the equivalent reaction is: 2 KC8H5O4 + 15 O2 + H2SO4 --> 16 CO2 + 6 H2O + K2SO4 Thus 2 mol of potassium acid phtalate consumes 15 mol of oxygen. The theoretical COD of potassium acid phtalate is 1,175 g of oxygen per gram of potassium acid phtalate. dapat 1,175 dapat dari: 15 O2 : 2 KC8H5O4 = 480 : 408 1,175 x 0,851 = 0,9999 g/L