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OF PLANT PATHOGENS:
A WHOLE DIFFERENT BIOCONTROL
GAME
ROBERT LARKIN
USDA-ARS, New England Plant, Soil, and Water Lab
Orono, ME
Biocontrol of plant pathogens and disease
is fundamentally different from biocontrol of
weeds, insects, or other animals. Emphasis is
placed on disease control rather than pathogen
control, and in some cases the pathogen is not
targeted at all. Thus, very different
characteristics are involved, and this biocontrol
only rarely resembles the traditional approach of
using debilitating pathogens or parasites to
destroy a target organism. These characteristics
have important implications on the potential
impacts of these biocontrol agents and
approaches.
Biocontrol Fungi for Control of Plant Pathogens
I. Introduction
V. Impacts
Distinctive Characteristics of Biocontrol of Plant
Pathogens
Diverse mechanisms of action
Competition
Antibiosis
Parasitism/Predation
Induced Resistance
Fo47
60
40
20
0
1 10 100 1,000 10,000 100,000
Biocontrol CFU/g soil
Larkin and Fravel, 1996
Approaches to minimize inundative applications
Site-specific applications
Seed Treatments
Transplant treatments
In-furrow application
Toxigenicity
Pathogenicity
Impacts
Displacement of nontarget organisms
Beneficial organisms
Population dynamics
Functional attributes
Structural attributes
BCA recovery
high root colonization
low soil populations
Soil Microbial Characteristics
Current Research - Biocontrol of Rhizoctonia
solani (stem canker and black scurf) on Potatoes
Other treatments:
Bacterial agents (Bacillus subtilis, Burkholderia cepacia)
Chemical seed treatment (Evolve)
Chemical/biological treatment (Evolve/Bcep)
Nontreated & Path-treated control
40
20
0
Soil fungi and Trichoderma populations
following biocontrol treatments (end of season)
CFU/g soil (x106 ) 60 Fungi
40
20 T.har
T.vir
0
3
a Trichoderma Evolve
CFU/g soil (x106 )
ab
2.5 No Path
ab
2 ab Path
1.5
b
1
0.5
0
Soil microbial activity following biocontrol
treatments (end of season)
0.6 T.har
a T.vir
ab
0.55
Optical Density
ab Evolve
ab
bc bc Ev/Bcep
0.5 c
c B.subtilis
B.cepacia
0.45
No Path
0.4 Path
Microbial activity
Soil Microbial Characteristics
(after one field season – potato crop)
Functional attributes
Substrate utilization assays
no consistent effects
chemical- lower for some substrate
groups
Structural attributes
FAME profiles
no overall effects
no major shifts in microbial communities
no change in biomarkers
Impacts
Toxigenicity (nontarget)
plants (phytotoxins)
other microorganisms
Pathogenicity
other crop plants
other microrganisms-
mycoparasites
CONCLUSIONS
Biocontrol of plant pathogens is distinctly different from
traditional biological control , involving different
mechanisms, effects, and approaches, resulting in different
challenges and different types of potential impacts.