The surface antigens of E. coli on which serotypes are based include the following: 1. O (somatic) – determined by the sugar side chains on the lipopolysaccharide molecule 2. K (capsular) – polysaccharide 3. H (flagellar) – proteinaceous 4. F (fimbrial) – proteinaceous; adhesive function Electron microscopy is a good method for definitive diagnosis of Chlamydophila. Diseases caused by Chlamydiaceae in animals and There are three morphologically distinct forms: man: 1. Elementary body (EB) – infectious form; 0.2-0.3 • Chlamydophila felis: endemic in cats; causes conjunctivitis with um diameter characterized by a highly electron blepharospasm, hyperemia, chemosis, ocular discharge and rhinitis. dense nucleoid at the periphery of the EB clearly Persistent genital and gastrointestinal infection may occur. separated from an electron dense cytoplasm • Chlamydophila psittaci: a common avian pathogen, a.k.a. Parrot 2. Reticulate body (RB) – intracellular, metabolically fever, Ornithosis, active form; 0.5-2.0 um diameter; lacy or reticular • Chlamydophila abortus: is endemic in ruminants causing abortion, nucleus, “hour-glass” profiles when undergoing especially in ewes, a.k.a. Enzootic Abortion of Ewes (EAE). Also binary fission affects rabbit, guinea pig, mice and human. 3. Intermediate body (IB) – 0.3-1.0 um diameter; • Chlamydophila caviae: specific pathogen of guinea pigs causing central electron dense core with radially arranged conjunctivitis. individual nucleoid fibers surrounding the core; • Chlamydophila pecorum: causes reproductive and urinary tract cytoplasmic granules tightly packed at the disease in Koalas. periphery of the IB separated from • Chlamydophila pneumoniae: a human pathogen causing bronchitis the core by a translucent zone and pneumonia, also affects frogs and snakes. • Chlamydia trachomatis: sexually transmitted human disease. In women, can infect the cervix, urethra and fallopian tubes resulting in Pelvic Inflammatory Disease (PID); in men, causes epididymitis. Also causes inflammation of the rectum, throat, and conjunctiva. Ocular infection in humans causes blindness and is called “trachoma”. A problem in developing countries primarily, affects 84 million people worldwide (5). • Chlamydia muridarum: pneumonia in mice and hamsters • Chlamydia suis: causes conjunctivitis, enteritis and pneumonia in swine. Diseases caused by Salmonella