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Morrel
Subject: biology
The Cell -
- Two different types of cell -
- Prokaryotic cells - Eukaryotic cells
- (anything made from it is - (anything made from it is
A prokaryotic organism) a eukaryotic organism)
- Theseare bacteria - found in animals and plants
- Cell ultra structure -
- Organelles - structures inside the cell -
#Chromosomes
- Usually in the form of chromatin
- Contains genetic information
- Composed of DNA
- Thicken for cellular division
- Set number per species (i.e. 23 pairs for human)
#Nuclear membrane
- Surrounds nucleus
- Composed of two layers
- Numerous openings for nuclear traffic
#Nucleolus
- Spherical shape
- Visible when cell is not dividing
- Contains RNA for protein manufacture
#Centrioles
- Paired cylindrical organelles near nucleus
- Composed of nine tubes, each with three tubules
- Involved in cellular division
- Lie at right angles to each other
#Chloroplasts
- A plastid usually found in plant cells
- Contain green chlorophyll where photosynthesis takes place
#Cytoskeleton
- Composed of microtubules
- Supports cell and provides shape
- Aids movement of materials in and out of cells
#Endoplasmic reticulum
- Tubular network fused to nuclear membrane
- Goes through cytoplasm onto cell membrane
- Stores, separates, and serves as cell's transport system
- Smooth type: lacks ribosome’s
- Rough type (pictured): ribosome’s embedded in surface
#Golgi apparatus
- Protein 'packaging plant'
- A membrane structure found near nucleus
- Composed of numerous layers forming a sac
#Lysosome
- Digestive 'plant' for proteins, lipids, and carbohydrates
- Transports undigested material to cell membrane for removal
- Vary in shape depending on process being carried out
- Cell breaks down if Lysosome explodes
#Mitochondria
- Second largest organelle with unique genetic structure
- Double-layered outer membrane with inner folds called cristae
- Energy-producing chemical reactions take place on cristae
- Controls level of water and other materials in cell
- Recycles and decomposes proteins, fats, and carbohydrates, and forms urea
#Ribosome’s
- Each cell contains thousands
- Miniature 'protein factories'
- Composes 25% of cell's mass
- Stationary type: embedded in rough endoplasmic reticulum
- Mobile type: injects proteins directly into cytoplasm
#Vacuoles
- Membrane-bound sacs for storage, digestion, and waste removal
- Contains water solution
- Contractile vacuoles for water removal (in unicellular organisms)
#Cell wall
- Most commonly found in plant cells
- Controls turgidity
- Extra cellular structure surrounding plasma membrane
- Primary cell wall: extremely elastic
- Secondary cell wall: forms around primary cell wall after growth is complete
#Plasma membrane
- Outer membrane of cell that controls cellular traffic
- Contains proteins (left, gray) that span through the membrane and allow passage of
materials
- Proteins are surrounded by a phospholipids bi-layer.
- Cell membrane -
- Outside the cell -
- Extrinsic membrane proteins - are imbedded in the outer phospholipids
layer. They often act as chemical receptors for the cell
- Intrinsic membrane proteins - pass right through the lipid layers and have
a variety of functions. Many transport molecules through the membrane either
into or out of the cell.
Facilitated diffusion - larger polar molecules and ions can diffuse but with
help, (similar diffusion but requires either carrier proteins or channel proteins)
Active transport -
- Movement of molecules against the concentration gradient
- Energy is required - provided by aerobic respiration
- Specific proteins in the membrane transport the molecules across
- Lowconcentration ------------> high concentration
Pascal’s)
Plant cell -
Animal cell -
Cardiac cycle -
- Oneheart beat is one cardiac cycle
- There are two-parts to a cardiac cycle-diastole (relaxation) and systole
(contraction)
- There is also -atrial diastole (relaxation of atria)
-and ventricular diastole (relaxation of ventricle)
- This is the same for systole - ventricular systole (contraction of ventricle)
- Atrial systole (contraction of a atria)
- Cardiac cycle - 1. Atrial diastole (relax) and ventricular diastole
2. Atrial systole (contraction) and ventricular diastole
3. Atrial diastole and ventricular systole
4. Atrial diastole and ventricular diastole
- Atrial diastole - atria relaxed and blood enters atria so it fills up
- Atrial systole - atria contract which increases blood pressure so bloods
forced through the bicuspid / tricuspid valve into ventricles.
- Ventricular systole - atrial diastole because no pressure so the ventricles
contract
Because of pressure and bicuspid/tricuspid vales close and the semi-lunar valves
open. Blood runs out of the heart into pulmonary and aorta arteries therefore the
semi lunar valves close to prevent backflow-ventricular diastole.
Heart sounds - lub blood hitting the closed AV valves during ventricular systole
(ATRIOVENTRICULAR)
Dub blood hitting the closed semi lunar valves during ventricular
Blood pressure (p) differences in the cardiac cycle -
Pa = pressure in atria Pv = pressure in ventricles Paorta = pressure in aorta
Respiratory system -
- Ventilation (breathing) - Movement of air in and out of the lungs
- Gaseous exchange- exchange of O2 and C02 between the blood and the
atmosphere across The AVEOLI of the lungs.
- Cellular respiration (AEROBIC) - production of energy (ANEROBIC respiration
produces lactic acid)
Need for ventilation system -
- To maintain concentration gradients across the alveoli
- Oxygen and carbon dioxide move across the alveoli by diffusion -
down the concentration gradient
- Inhalation increases oxygen gradient from the alveolus to blood
- Exhalation decreases carbon dioxide gradient from the blood to
alveolus
Features of alveoli for gaseous exchange -
- Large surface area (for increased diffusion)
- Single layer of flattened cells (for more diffusion)
- Moist lining (for diffusion to occur)
- Dense network of blood capillaries
Fick’s law - rate of diffusion = surface area (large) x difference in concentration
(large)
Thickness of the membrane (thin)
Mechanism of inhalation -
- External intercostals muscles contract.
- causing ribcage to move up and out.
- Diaphragm contracts and flattens.
- Abdominal muscles relax.
- Volume of thorax increases-decreases in pressure in thoracic cavity
pressure.
- Air moves into lungs.
Mechanism of exhalation -
- External intercostals muscles relax.
- Causing ribcage to move down and inwards.
- Diaphragm relaxes and becomes dome shaped.
- Abdominal muscles contract.
- Volume of thorax decreases-increases pressure in thoracic cavity
pressure.
- Air moves out of lungs
Tidal volume – volume of air inhaled and exhaled during normal breathing.
(Normal ½ litres)
Vital capacity – maximum volume of air that can be expelled.
Residual volume – volume of air left in lungs after deep exhalation.
Pulmonary ventilation – the total volume of air inhaled / minute (normally 15
times a minute)
Pulmonary ventilation rate = tidal volume x breathing rate. (Normally around 7½
litres)
Dm3min-1 dm3 min-1
Control of breathing rate (control of breathing rate) –
Microscopes -
2 advantages and disadvantages of an electron microscope –
- An electron microscope has better resolution than a light microscope
- An electron microscope has better magnification so more organelles are
discovered
- An electron microscope is very expensive
- An electron microscope kills the cells during this process
Cell fractionation/homogenization – the process in which cells are split apart
to see the details in the cells.
Homogenization – cells are broken down in a homogenizer so that the cell
membrane is broken and not the organelles so then you can look at the cell
insides with detail.
Centrifugation – when the liquid is spun after homogenization then the larger
molecules accumulate towards the bottom of the test tube.
MODULE 2 - syllabus -
The Cell cycle -
General life cycle -
1. Mitosis – produces genetically identical cells (daughter cells) that have the
same number of chromosomes. 2N -> 2N + 2N -> 2N + 2N + 2N + 2N etc.
Functions: growth, repair of tissues, replace of cells, asexual reproduction
2. Meiosis – division of the nucleus to produce daughter nuclei (cells) with half the
number of chromosomes (haploid) 2N -> N + N (meiosis 1)-> N + N + N + N
(meiosis 2) – end up with 4 haploid gametes. The daughter nuclei are genetically
different.
Function: production of gametes for sexual production.
4 stages of mitosis -
Mitosis practical -
The Immune response –
- Physico-chemical barriers –
Skin (protective barrier),
Mucus membranes (trap bacteria etc),
Nasal hairs (traps bacteria),
Cilia (waft up mucus),
Saliva (lysozyme – enzyme breaks down bacterial cell walls),
Tears (same as saliva but in eyes),
HCL in stomach (breaks down stuff),
Blood Clotting (platelets etc clots to make scabs over cuts).
- Lymphocytes produce antibodies and they are responsible for specific immune
response.
- Two main types – T-cells – responsible for the cell mediated response - identify
- B-cells – responsible for the production of antibodies – gets
response
Principles of immunology –
- Antigens – antigens are large, organic molecules found on the surface of the cell
membrane. Every individual has their own unique cell surface molecules, which
the immune system recognizes. So when a pathogen like a bacterium invades the
body, the antigens on its cell surface are identified as foreign by the immune
system.
- Blood grouping – red blood cells (Erythrocytes) carry special antigens (called
agglutinogens) on their surface. There are two types of red blood cell antigen – A
and B. These determine Blood groups – a person with type A antigens will be
blood group A, a person with type A and B antigens = blood group AB and so on…
If you don’t have any antigens on your red blood cells your blood group O.
Blood plasma caries special antibodies called agglutinogens, again there are two
types – A and B. If blood antigens and antibodies of the same type mix,
agglutinogen occurs – the agglutinins bind to the agglutinogens on the blood cells,
forming a clump. This can be fatal because the clumps can block arteries.
So we don’t have agglutinogens and agglutinins of the same type in our bodies,
if we have type A agglutinogens, we’ll have type B agglutinins and so on…
Where do you find anti-B and anti-A antibodies? – They are found in the blood
plasma. The antibodies are specific to the blood type.
Genetic fingerprinting/profiling –
- DNA cut into fragments of different lengths using restriction enzymes.
- DNA is placed on a gel and on electric field applied called electrophoresis.
- The DNA fragments move towards the positive electrode
- smaller fragments move faster, separating the fragments of different lengths
- labelled gene probes are used to locate various base sequences along the
fragments
- Gene probes form patterns on photographic plate -
Adaptations of cereal -
Respiration
– two types :
– 1. aerobic (requires oxygen). This is 19 times more efficient than anaerobic.
- 2. anaerobic (low oxygen concentration). This makes lactic acid and ethanol
which is toxic.
Artificial environments –
- Famers can help the plants grow by giving optimum conditions (above).
Questions –
1. Two ways that rice is adapted to live in swampy fields are because it can
respirate anaerobicaly and it has air spaces called aerenchyma
2. Four ways in which sorghum is adapted structurally is it has long roots for
deep penetration of the soil, It has shrunken stomata, It can role its leaves
and it has waxy cuticles.
3. Maize reduces its water loss by having closed stomata
Fertilisers –
These replace nutrients lost from the soil. These are nitrates (protein for growth
and repair etc), phosphates (DNA)and potassium.
Leaching of the soil is washing out of the minerals from the soil
Eutrophication is when fertilizer soaks into rivers from the soil so it contaminates
it.