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BACTERIAL CONJUGATION

BACTERIAL CONJUGATION

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Published by: cnmi on Jul 28, 2008
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04/19/2013

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BACTERIALBACTERIALCONJUGATIONCONJUGATION
 
 
 
INTRODUCTIONINTRODUCTION
Bacteria posses basic three mechanisms of lateral or horizontal geneBacteria posses basic three mechanisms of lateral or horizontal genetransfer. They are,transfer. They are,
 Transformation- transfer of gene from one bacterium to other Transformation- transfer of gene from one bacterium to otherwithout cell to cell contact.without cell to cell contact.
 Transduction– transfer of gene from one bacterium to other via Transduction– transfer of gene from one bacterium to other viaagency of phages.agency of phages.
Conjugation- transfer of gene from one bacterium to other by cellConjugation- transfer of gene from one bacterium to other by cellto cell contact by conjugal pore.to cell contact by conjugal pore.Conjugation was first discovered in 1946 by two renownedConjugation was first discovered in 1946 by two renownedscientists of the timescientists of the time Joshua LederbergandEdward Tatum ..Bacterial conjugation is often incorrectly regarded as thebacterialequivalentequivalent of sexual reproduction or mating.BacterialBacterialconjugation is merely a transfer of gene from one bacterium toconjugation is merely a transfer of gene from one bacterium toother unlike the fusion of gametes in sexual reproduction in higherother unlike the fusion of gametes in sexual reproduction in higherorganisms.organisms.
 
 
DISTINGUISHING CHARACTERISTICS OFDISTINGUISHING CHARACTERISTICS OFCONJUGATIONCONJUGATION
DNA transfer requires cell-cell contact.DNA transfer requires cell-cell contact.
DNA transfer occurs via a conjugal pore.DNA transfer occurs via a conjugal pore.
DNA transfer occurs in one direction - from donor toDNA transfer occurs in one direction - from donor torecipient not vice versarecipient not vice versa
DNA transfer does not require protein synthesis in donor.DNA transfer does not require protein synthesis in donor.
DNA transfer requires energy in donor cell - primarily ATP.DNA transfer requires energy in donor cell - primarily ATP.

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