Alleleustrious pmWasabi-ELP-1

Catalog Number: Introduction All ustrious pmWasabi-ELP-1 is a mammalian expression vector that encodes a fusion of human ELP-1 (ERD-like polypeptide, also known as KDEL receptor-2 or KDELR2) N-terminal to mWasabi. This product can be used as a subcellular marker for the lumen of the endoplasmic reticulum (ER). All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. ELP-1 or ERD2 is a seven-transmembrane protein that functions as a receptor for the signal peptide lys-asp-glu-leu (KDEL). Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved by their continual retrieval from the cis-Golgi, or pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal KDEL in animal cells. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER.
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ABP-FP-WELP Source Engineered variant of mTFP1, originally derived from Clavularia sp. coral. Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 ELP-1..........................................612-1247 Linker......1248-1322 Wasabi.1323-2033 SV40 PolyA Signal.....................2185-2235 bla Promoter...............................2774-2878 Ampicillin Resistance Gene......2863-3723 pUC Origin..................................3872-4514 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A Wasabi AMPR pUC Ori PCMV

pmWasabiELP-1 4596bp

ELP-1

Linker

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Hsu,V.W., Shah,N. and Klausner,R.D. (1992). "A brefeldin A-like phenotype is induced by the overexpression of a human ERD-2-like protein, ELP-1" Cell 69 (4), 625-635. van der Vlies,D., Pap,E.H., Post,J.A., Celis,J.E. and Wirtz,K.W. (2002). "Endoplasmic reticulum resident proteins of normal human dermal fibroblasts are the major targets for oxidative stress induced by hydrogen peroxide " Biochem. J. 366 (PT 3).

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-ELP-1
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCACCATGGC TCATCCTGCT AGCTTCTGTT CATTGTATAA TGATCTACCT TTCTGGTGGT AGATCCTCTG TGATCAGCAA TCTATCGTGC ACCTCATTGC TGTACATTAC AGCTCAAGCT CCATGGTGAG AGCTGAAGAT GCAAGCCCTA CCTTCTCCTA ACCCCGACGA GCACCATGAC AGGACTCCTT TGATGCAGAA GCGTGCTGAA TTGACTTCAA TGGACCACCG AGATCGCCGT CGACTCTAGA CTCCCACACC TTTATTGCAG GCATTTTTTT GTCCTGTAGC ACTTGCCAGC CGCCGGCTTT TTTACGGCAC GCCCTGATAG CTTGTTCCAA GATTTTGCCG GAATTTTAAC GGAACCCCTA TAACCCTGAT CGTGTCGCCC ACGCTGGTGA CTGGATCTCA ATGAGCACTT GAGCAACTCG ACAGAAAAGC ATGAGTGATA ACCGCTTTTT CTGAATGAAG ACGTTGCGCA GACTGGATGG TGGTTTATTG CTGGGGCCAG ACTATGGATG TAACTGTCAG TTTAAAAGGA GAGTTTTCGT CCTTTTTTTC GTTTGTTTGC GCGCAGATAC TCTGTAGCAC GGCGATAAGT CGGTCGGGCT GAACTGAGAT GCGGACAGGT GGGGGAAACG TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA CATGAACATT GCTGAAGATC TGCACTGGTC CACATCTATG GAAATTTAAG CCCTGTGGGA GACCTTCTCC GACTGGGGAG TTTGTATCTT TGTGGTGGCC AAAAGTACTC TCGAATTCTG CAAGGGCGAG GGAGGGCAAC CGACGGCACC CGACATTCTG CATCCCCAAC CTTCGAGGAC CATCTACGAG GGAGACCACC GGGCGACGTC GACCATCTAC CATCGAGATC GGCCCGCAAC TCATAATCAG TCCCCCTGAA CTTATAATGG CACTGCATTC GGCGCATTAA GCCCTAGCGC CCCCGTCAAG CTCGACCCCA ACGGTTTTTC ACTGGAACAA ATTTCGGCCT AAAATATTAA TTTGTTTATT AAATGCTTCA TTATTCCCTT AAGTAAAAGA ACAGCGGTAA TTAAAGTTCT GTCGCCGCAT ATCTTACGGA ACACTGCGGC TGCACAACAT CCATACCAAA AACTATTAAC AGGCGGATAA CTGATAAATC ATGGTAAGCC AACGAAATAG ACCAAGTTTA TCTAGGTGAA TCCACTGAGC TGCGCGTAAT CGGATCAAGA CAAATACTGT CGCCTACATA CGTGTCTTAC GAACGGGGGG ACCTACAGCG ATCCGGTAAG CCTGGTATCT TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG TTCCGGCTGA TGGAAGACGC TTCACAACTC AAGGTTATCT GCAACCTACG GGCCTCTCAT ATCTACCTGG GCCGAGACCA GTCAACTGGA GGCGTAGTCC AAGGGAAAGA CAGTCGACGG GAGACCACAA GTGAATGGCC AACACCATCA ACCACCGCGT TACTTCAAGC AAGGGCATCG ATACACCTCA GGCTGGGACG AAGATGAAGC AGGGCCAAGA CTGAACCACG TCCACCGACG CCATACCACA CCTGAAACAT TTACAAATAA TAGTTGTGGT GCGCGGCGGG CCGCTCCTTT CTCTAAATCG AAAAACTTGA GCCCTTTGAC CACTCAACCC ATTGGTTAAA CGCTTACAAT TTTCTAAATA ATAATATTGA TTTTGCGGCA TGCTGAAGAT GATCCTTGAG GCTATGTGGC ACACTATTCT TGGCATGACA CAACTTACTT GGGGGATCAT CGACGAGCGT TGGCGAACTA AGTTGCAGGA TGGAGCCGGT CTCCCGTATC ACAGATCGCT CTCATATATA GATCCTTTTT GTCAGACCCC CTGCTGCTTG GCTACCAACT TCTTCTAGTG CCTCGCTCTG CGGGTTGGAC TTCGTGCACA TGAGCTATGA CGGCAGGGTC TTATAGTCCT ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC CTGGGGACCT GCTCCTGCGC GTTACCTGGA ACCTTGCCTG ATGGAAATCA TTTTAGTTAA AGTCCGTGGC TCACCACCCA TCTGGCGCTT AGACCATCCT AGCTCAGTTT TACCGCGGGC TGGGCGTAAT ACGCCTTCGT ACCTGGAGGT TCAGTTACGG AGTCCTTCCC TGAAGGTGAA AGGGCGAGAA CCTCCACCGA TGCTGCTGGA AGGCGGTGAA ACAAGGACTA GCATGGACGA TTTGTAGAGG AAAATGAATG AGCAATAGCA TTGTCCAAAC TGTGGTGGTT CGCTTTCTTC GGGGCTCCCT TTAGGGTGAT GTTGGAGTCC TATCTCGGTC AAATGAGCTG TTAGGTGGCA CATTCAAATA AAAAGGAAGA TTTTGCCTTC CAGTTGGGTG AGTTTTCGCC GCGGTATTAT CAGAATGACT GTAAGAGAAT CTGACAACGA GTAACTCGCC GACACCACGA CTTACTCTAG CCACTTCTGC GAGCGTGGGT GTAGTTATCT GAGATAGGTG CTTTAGATTG GATAATCTCA GTAGAAAAGA CAAACAAAAA CTTTTTCCGA TAGCCGTAGT CTAATCCTGT TCAAGACGAT CAGCCCAGCT GAAAGCGCCA GGAACAGGAG GTCGGGTTTC AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC GTCCCACCTG CGGTATTTCT TCTTTTTACT CTCCTATGCC TGATACCTTC TCACGATTTC TATCCTTCCG CTACCTGTTC CTACTTTGAG ATACTGTGAC GCCAGCAGGC CCGGGATCCA CAAGCCCGAC GATCGAGGGC GAAGGAGGGA CAACAGGGCC CGAGGGCTAC GTCCGACATC CTTCCCCCCC GAGGATGTAC GGGCGGCGGC GCTGCCCGAC CAACAAGGTG GCTGTACAAG TTTTACTTGC CAATTGTTGT TCACAAATTT TCATCAATGT ACGCGCAGCG CCTTCCTTTC TTAGGGTTCC GGTTCACGTA ACGTTCTTTA TATTCTTTTG ATTTAACAAA CTTTTCGGGG TGTATCCGCT GTATGAGTAT CTGTTTTTGC CACGAGTGGG CCGAAGAACG CCCGTATTGA TGGTTGAGTA TATGCAGTGC TCGGAGGACC TTGATCGTTG TGCCTGTAGC CTTCCCGGCA GCTCGGCCCT CTCGCGGTAT ACACGACGGG CCTCACTGAT ATTTAAAACT TGACCAAAAT TCAAAGGATC AACCACCGCT AGGTAACTGG TAGGCCACCA TACCAGTGGC AGTTACCGGA TGGAGCGAAC CGCTTCCCGA AGCGCACGAG GCCACCTCTG TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCCG GCGGCCATCG GGGAAAAGCC TCATTTATTT ACAGTGTACC CGAGTGGAGT TCTCCTCTTG CAGCTGTTTA TTCCTGGGCC GGCTTCTTTG TTCTTCTACT GGTGGGGGGG CCGGTCGCCA ATGAAGATCA GAGGGCGAGG GCCCCCCTGC TTCACCAAGT TCTTGGGAGC TCCATGGAGG AACGGCCCCG GTGCGCGACG CACCACCGCG TATCACTTTG ACCGTTTACG TAAGCGGCCG TTTAAAAAAC TGTTAACTTG CACAAATAAA ATCTTAACGC TGACCGCTAC TCGCCACGTT GATTTAGTGC GTGGGCCATC ATAGTGGACT ATTTATAAGG AATTTAACGC AAATGTGCGC CATGAGACAA TCAACATTTC TCACCCAGAA TTACATCGAA TTTTCCAATG CGCCGGGCAA CTCACCAGTC TGCCATAACC GAAGGAGCTA GGAACCGGAG AATGGCAACA ACAATTAATA TCCGGCTGGC CATTGCAGCA GAGTCAGGCA TAAGCATTGG TCATTTTTAA CCCTTAACGT TTCTTGAGAT ACCAGCGGTG CTTCAGCAGA CTTCAAGAAC TGCTGCCAGT TAAGGCGCAG GACCTACACC AGGGAGAAAG GGAGCTTCCA ACTTGAGCGT

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-ELP-1
4441 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 4501 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 4561 CCTGATTCTG TGGATAACCG TATTACCGCC ATGCAT

Enzyme AatI AatII Acc113I Acc16I Acc65I AccB1I AccB7I AccBSI AccI AcsI AcyI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbsI Bbv12I Bbv16II BcgI BclI BcoI BglI BglII BpiI BpmI Bpu14I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseRI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsmI BsoBI Bsp119I Bsp120I Bsp1407I Bsp143II Bsp19I BspCI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI

Cuts 1 5 2 1 1 5 1 2 1 5 7 2 1 1 4 2 2 2 1 2 5 2 2 2 1 1 4 2 1 2 1 5 4 7 2 4 2 1 1 2 6 1 2 4 1 2 3 2 7 4 2 5 4 6 2 3 5 2 5 5 4 2 2 2 1 1 2 5 4 1 3 1 2 2 3 6 2 6 2 4 1 2 7 1 5 1

Positions 922 125 178 261 447 1819 1216 3169 3427 1289 465 1289 1455 2466 3697 1762 2366 2811 1284 852 1273 2205 2690 2701 122 175 258 444 1768 1816 3110 2277 4538 1310 3650 1264 2982 3067 4228 2978 4224 725 4129 1300 1599 1301 2978 4224 852 1273 2205 2690 2701 7 3475 7 3475 618 3050 1289 3650 1264 2982 3067 4228 1300 1599 3427 609 1408 1304 465 1289 1455 2466 3697 1264 1301 1493 2436 122 175 258 444 1768 1816 3110 688 743 1264 2982 3067 4228 688 743 3134 1021 1300 1599 90 212 283 606 1890 3532 676 688 743 992 1478 1841 3565 1271 688 743 340 1790 2507 874 2053 122 175 258 444 1768 1816 3110 1049 1346 1757 3583 2141 2240 1315 2038 3132 3281 4204 1310 3354 4185 4332 699 1158 1310 1758 2402 3565 874 2053 957 1343 1682 1315 2038 3132 3281 4204 874 2053 465 1289 1455 2466 3697 1315 2038 3132 3281 4204 1264 2982 3067 4228 2986 4370 2141 2240 1300 1599 1271 1297 1201 2023 598 1118 2352 2360 4298 360 604 1321 1672 3281 878 2810 3818 4538 2366 2811 874 2053 1150 3414 3596 699 1158 1310 1758 2402 3565 1201 2023 699 1158 1310 1758 2402 3565 2986 4370 360 604 1321 1672 1271 2366 2811 360 604 994 1293 1321 1672 1987 1967 598 1118 2352 2360 4298 1304

Sequence agg/cct gacgt/c agt/act tgc/gca g/gtacc g/gyrcc ccannnn/ntgg gagcgg gt/mkac r/aatty gr/cgyc a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg gggcc/c g/tgcac r/aatty at/taat at/taat gaann/nnttc g/gtacc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc gaagac gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc a/gatct gaagac ctggag tt/cgaa gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc gaggag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg gaatgc c/ycgrg tt/cgaa g/ggccc t/gtaca rgcgc/y c/catgg cgat/cg t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc

BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI CciNI Cfr10I Cfr42I Cfr9I CfrI Csp45I DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco147I Eco24I Eco255I Eco31I Eco52I Eco57I Eco64I Eco88I Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I ErhI Esp1396I FauNDI FbaI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I KpnI Ksp22I Ksp632I KspI LspI MamI MfeI MflI MluI MluNI Mph1103I MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI

5 1 6 1 13 2 13 1 1 6 1 1 7 1 4 2 2 2 7 7 1 1 1 1 1 1 1 1 4 1 4 2 4 1 5 5 2 1 1 1 2 1 1 4 1 4 1 1 1 4 1 4 5 7 4 4 1 1 7 1 1 1 1 1 2 1 13 1 2 1 2 2 9 7 5 1 2 2 4 1 2 2 1

1315 2038 3132 3281 4204 1967 1278 1877 2284 3404 4082 4273 340 585 676 758 961 1108 1304 1936 3003 3020 3788 3800 3886 3897 986 1472 585 676 758 961 1108 1304 1936 3003 3020 3788 3800 3886 3897 2035 2035 699 1158 1310 1758 2402 3565 1296 1300 607 652 1156 1406 1847 2035 3257 1271 2093 3072 3764 3783 635 1546 1919 2510 2554 4436 360 604 994 1293 1321 1672 1987 607 652 1156 1406 1847 2035 3257 2035 2861 3650 2861 1262 3650 2035 340 360 604 1321 1672 922 1264 1301 1493 2436 1216 3169 1049 1346 1757 3583 2035 677 1388 1811 2968 4016 465 1289 1455 2466 3697 1300 1599 1967 1262 916 635 1546 1967 1273 360 604 1321 1672 4595 360 604 1321 1672 1762 234 1021 1264 1301 1493 2436 3427 992 1478 1841 3565 598 1118 2352 2360 4298 122 175 258 444 1768 1816 3110 1103 1285 1862 2154 1103 1285 1862 2154 1266 2154 122 175 258 444 1768 1816 3110 1293 1021 2861 1296 1271 874 2053 2141 585 676 758 961 1108 1304 1936 3003 3020 3788 3800 3886 3897 2277 609 1408 4595 1158 2402 609 1408 365 609 795 1149 1323 1671 2879 3238 3397 122 175 258 444 1768 1816 3110 1013 1295 3014 3955 4200 2141 2141 2240 1160 2404 360 604 1321 1672 234 1158 2402 1158 2402 591

cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg gc/ggccgc r/ccggy ccgc/gg c/ccggg y/ggccr tt/cgaa ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg agg/cct grgcy/c agt/act ggtctc c/ggccg ctgaag g/gyrcc c/ycgrg g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg t/gatca grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc ggtac/c t/gatca ctcttc ccgc/gg tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-ELP-1
NotI NsiI NspBII NspI NspV PflMI PinAI Ple19I Pme55I Ppu10I PpuMI PshBI Psp124BI Psp1406I Psp5II PspAI PspALI PspEI PspOMI PstI PstNHI PvuI PvuII RcaI SacI 1 1 5 1 1 1 1 1 1 1 1 2 1 2 1 1 1 1 1 1 1 1 1 3 1 2035 4595 1013 1295 3014 3955 4200 4542 1271 1762 1310 3281 922 4591 635 7 3475 1264 3048 3421 635 1300 1302 1967 1297 1282 591 3281 1013 878 2810 3818 1264 gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa ccannnn/ntgg a/ccggt cgat/cg agg/cct a/tgcat rg/gwccy at/taat gagct/c aa/cgtt rg/gwccy c/ccggg ccc/ggg g/gtnacc g/ggccc ctgca/g g/ctagc cgat/cg cag/ctg t/catga gagct/c SacII SalI ScaI SfcI Sfr303I SfuI SmaI SnaBI SseBI SspBI SspI SstI SstII StuI StyI Van91I VneI VspI XbaI XcmI XhoII XmaI XmaIII XmnI Zsp2I 1 1 2 6 1 1 1 1 1 2 2 1 1 1 4 1 2 2 1 1 13 1 1 2 1 1296 1283 1216 3169 1278 1877 2284 3404 4082 4273 1296 1271 1302 340 922 1201 2023 2715 2845 1264 1296 922 360 604 1321 1672 1762 2978 4224 7 3475 2045 2009 585 676 758 961 1108 1304 1936 3003 3020 3788 3800 3886 3897 1300 2035 618 3050 4595 ccgc/gg g/tcgac agt/act c/tryag ccgc/gg tt/cgaa ccc/ggg tac/gta agg/cct t/gtaca aat/att gagct/c ccgc/gg agg/cct c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg r/gatcy c/ccggg c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: AatI, Acc65I, AclNI, AflII, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbuI, BfrI, BlnI, Bsa29I, BscI, BseCI, BsePI, BsiWI, BsmBI, Bsp106I, Bsp120I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, Bsu15I, Cfr42I, Cfr9I, ClaI, CpoI, CspI, Eco147I, Eco32I, Eco72I, EcoRV, EheI, Esp3I, FseI, KasI, KpnI, KspI, MspCI, NarI, NruI, PacI, PaeI, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp5II, PspAI, PspALI, PspLI, PspOMI, RsrII, SacII, SbfI, SexAI, SfiI, Sfr303I, SgfI, SgrAI, SmaI, SmiI, SpeI, SphI, SplI, SrfI, Sse8387I, SseBI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XmaI

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010