Alleleustrious pmTFP1-Vimentin

Catalog Number: Introduction All ustrious pmTFP1-Vimentin is a mammalian expression vector that expresses mTFP1 fused to the C-terminus of a human vimentin (VIM). This product can be a great tool for your research involving cytoskeleton (intermediate filaments). The properties of All ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in the mitotic spindle of mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. Vimentin forms intermediate filaments. Along with the microfilaments (actins) and microtubules (tubulins), Vimentins represent a third class of well-characterized cytoskeletal elements. The subunits of intermediate filaments display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure. It is found that vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol.
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ABP-FP-TVIMB100

Source Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features More than 3-fold brighter than ECFP More than twice as photostable as ECFP Single emission peak Single-exponential fluorescence lifetime ( = 3.2 ns) Can be co-imaged with red and yellow FPs Low sensitivity to acidic pH (fluorescence pKa=4.3) Mammalian expression vector ready to transfect your favorite cells True monomer that will not aggregate or cause non-specific interactions Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Vimentin.....................................637-2034 Linker......2035-2055 mTFP1.2056-2766 SV40 PolyA Signal.....................2918-2968 bla Promoter...............................3507-3611 Ampicillin Resistance Gene......3596-4456 pUC Origin..................................4605-5247 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A mTFP1 AMPR pUC Ori PCMV

pmTFP1Vimentin 5329bp

Vimentin

Linker

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. Goldman R. D., Khuon S., Chou Y., Opal P., Steinert P. "The function of intermediate filaments in cell shape and cytoskeletal integrity" J Cell Biol 1996 134 (4): pp. 97183. Katsumoto T., Mitsushima A., Kurimura T.. "The role of the vimentin intermediate filaments in rat 3Y1 cells elucidated by immunoelectron microscopy and computer-graphic reconstruction" Biol Cell 68 1990 (2): pp. 13946.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Vimentin
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG TCCTCCTACC AGCTACGTGA AGCCGCAGCC CGCCTGCGGA GCCGACGCCA GAGCTGAATG AAGATCCTGC CTCTACGAGG GCCCGCGTCG TTGCAGGAGG GATGTTGACA GAAGAGATTG ATTCAGGAAC CTGCGTGACG GAATGGTACA CTGCGCCAGG GAAGTGGATG GAGAACTTTG ATTCAGAATA GTTAAGATGG AGCAGGATTT GATTCACTCC ACTAGAGATG CCACCGGTCG GACATGAAGA GGCGAGGGCG GGAGCCCCCC GCCTTCACCA TACTCTTGGG ATCTCCATGG CCCAACGGCC TACGTGCGCG GGCCACCACC GACTATCACT GTGACCGTTT AAGTAAGCGG TGCTTTAAAA TGTTGTTAAC TTTCACAAAT TGTATCTTAA GCGTGACCGC TTCTCGCCAC TCCGATTTAG GTAGTGGGCC TTAATAGTGG TTGATTTATA AAAAATTTAA GGGAAATGTG GCTCATGAGA TATTCAACAT TGCTCACCCA GGGTTACATC ACGTTTTCCA TGACGCCGGG GTACTCACCA TGCTGCCATA ACCGAAGGAG TTGGGAACCG AGCAATGGCA GCAACAATTA CCTTCCGGCT TATCATTGCA GGGGAGTCAG TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT GCAGGATGTT CTACGTCCAC TCTACGCCTC GCAGCGTGCC TCAACACCGA ACCGCTTCGC TGGCCGAGCT AGGAGATGCG AGGTGGAGCG AGATGCTTCA ATGCGTCTCT CCTTTTTGAA AGCATGTCCA TACGTCAGCA AATCCAAGTT CAAAGCAGGA CCCTTAAAGG CCGTTGAAGC TGAAGGAGGA CCCTTGACAT CTCTGCCTCT CTCTGGTTGA GACAGGTTAT CCACCATGGT TCAAGCTGAA AGGGCAAGCC TGCCCTTCTC AGTACCCCGA AGCGCACCAT AGGAGGACTC CCGTGATGCA ACGGCGTGCT GCGTTGACTT TTGTGGACCA ACGAGAGCGC CCGCGACTCT AACCTCCCAC TTGTTTATTG AAAGCATTTT CGCGTCCTGT TACACTTGCC GTTCGCCGGC TGCTTTACGG ATCGCCCTGA ACTCTTGTTC AGGGATTTTG CGCGAATTTT CGCGGAACCC CAATAACCCT TTCCGTGTCG GAAACGCTGG GAACTGGATC ATGATGAGCA CAAGAGCAAC GTCACAGAAA ACCATGAGTG CTAACCGCTT GAGCTGAATG ACAACGTTGC ATAGACTGGA GGCTGGTTTA GCACTGGGGC GCAACTATGG TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGA CGGCGGCCCG CCGCACCTAC GTCCCCGGGC CGGGGTGCGG GTTCAAGAAC CAACTACATC CGAGCAGCTC GGAGCTGCGC CGACAACCTG GAGAGAGGAA GGCACGTCTT GAAACTCCAC AATCGATGTG ATATGAAAGT TGCTGACCTC GTCCACTGAG AACCAATGAG TGCTAACTAC AATGGCTCGT TGAGATTGCC TCCAAACTTT TACCCACTCA CAACGAAACT GAGCAAGGGC GATGGAGGGC CTACGACGGC CTACGACATT CGACATCCCC GACCTTCGAG CTTCATCTAC GAAGAAGACC GAAGGGCGAC CAAGACCATC CCGCATCGAG CGTGGCCCGC AGATCATAAT ACCTCCCCCT CAGCTTATAA TTTCACTGCA AGCGGCGCAT AGCGCCCTAG TTTCCCCGTC CACCTCGACC TAGACGGTTT CAAACTGGAA CCGATTTCGG AACAAAATAT CTATTTGTTT GATAAATGCT CCCTTATTCC TGAAAGTAAA TCAACAGCGG CTTTTAAAGT TCGGTCGCCG AGCATCTTAC ATAACACTGC TTTTGCACAA AAGCCATACC GCAAACTATT TGGAGGCGGA TTGCTGATAA CAGATGGTAA ATGAACGAAA ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ATTCCCATGT GGCACCGCGA AGCCTGGGCA GGCGTGTATG CTCCTGCAGG ACCCGCACCA GACAAGGTGC AAGGGCCAAG CGGCAGGTGG GCCGAGGACA GCCGAAAACA GACCTTGAAC GAAGAGGAAA GATGTTTCCA GTGGCTGCCA TCTGAGGCTG TACCGGAGAC TCCCTGGAAC CAAGACACTA CACCTTCGTG ACCTACAGGA TCCTCCCTGA AAAAGGACAC TCTCAGCATC GAGGAGACCA AACGTGAATG ACCAACACCA CTGACCACCG AACTACTTCA GACAAGGGCA GAGATACACC ACCGGCTGGG GTCAAGCACA TACAGGGCCA ATCCTGAACC AACTCCACCG CAGCCATACC GAACCTGAAA TGGTTACAAA TTCTAGTTGT TAAGCGCGGC CGCCCGCTCC AAGCTCTAAA CCAAAAAACT TTCGCCCTTT CAACACTCAA CCTATTGGTT TAACGCTTAC ATTTTTCTAA TCAATAATAT CTTTTTTGCG AGATGCTGAA TAAGATCCTT TCTGCTATGT CATACACTAT GGATGGCATG GGCCAACTTA CATGGGGGAT AAACGACGAG AACTGGCGAA TAAAGTTGCA ATCTGGAGCC GCCCTCCCGT TAGACAGATC AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC CCACCAGGTC GCCGGCCGAG GCGCGCTGCG CCACGCGCTC ACTCGGTGGA ACGAGAAGGT GCTTCCTGGA GCAAGTCGCG ACCAGCTAAC TCATGCGCCT CCCTGCAATC GCAAAGTGGA TCCAGGAGCT AGCCTGACCT AGAACCTGCA CCAACCGGAA AGGTGCAGTC GCCAGATGCG TTGGCCGCCT AATACCAAGA AGCTGCTGGA ACCTGAGGGA TTCTGATTAA ACGATGACCT CAATGGGCGT GCCACGCCTT TCAACCTGGA CGTTCGCCTA AGCAGTCCTT TCGTGAAGGT TCAAGGGCGA ACGCCTCCAC AGCTGCTGCT AGAAGGCGGT ACGACAAGGA ACGGCATGGA ACATTTGTAG CATAAAATGA TAAAGCAATA GGTTTGTCCA GGGTGTGGTG TTTCGCTTTC TCGGGGGCTC TGATTAGGGT GACGTTGGAG CCCTATCTCG AAAAAATGAG AATTTAGGTG ATACATTCAA TGAAAAAGGA GCATTTTGCC GATCAGTTGG GAGAGTTTTC GGCGCGGTAT TCTCAGAATG ACAGTAAGAG CTTCTGACAA CATGTAACTC CGTGACACCA CTACTTACTC GGACCACTTC GGTGAGCGTG ATCGTAGTTA GCTGAGATAG TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CGTGTCCTCG CTCCAGCCGG CCCCAGCACC CTCTGCCGTG CTTCTCGCTG GGAGCTGCAG GCAGCAGAAT CCTGGGGGAC CAACGACAAA CCGGGAGAAA TTTCAGACAG ATCTTTGCAA GCAGGCTCAG CACGGCTGCC GGAGGCAGAA CAATGACGCC CCTCACCTGT TGAAATGGAA GCAGGATGAG CCTGCTCAAT AGGCGAGGAG AACTAATCTG GACGGTTGAA TGAAGGGGAT AATCAAGCCC CGTGATCGAG GGTGAAGGAG CGGCAACAGG CCCCGAGGGC GAAGTCCGAC GAACTTCCCC CGAGAGGATG GGAGGGCGGC GAAGCTGCCC CTACAACAAG CGAGCTGTAC AGGTTTTACT ATGCAATTGT GCATCACAAA AACTCATCAA GTTACGCGCA TTCCCTTCCT CCTTTAGGGT GATGGTTCAC TCCACGTTCT GTCTATTCTT CTGATTTAAC GCACTTTTCG ATATGTATCC AGAGTATGAG TTCCTGTTTT GTGCACGAGT GCCCCGAAGA TATCCCGTAT ACTTGGTTGA AATTATGCAG CGATCGGAGG GCCTTGATCG CGATGCCTGT TAGCTTCCCG TGCGCTCGGC GGTCTCGCGG TCTACACGAC GTGCCTCACT

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Vimentin
4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 5281 GATTAAGCAT ACTTCATTTT AATCCCTTAA ATCTTCTTGA GCTACCAGCG TGGCTTCAGC CCACTTCAAG GGCTGCTGCC GGATAAGGCG AACGACCTAC CGAAGGGAGA GAGGGAGCTT CTGACTTGAG CAGCAACGCG TCCTGCGTTA TGGTAACTGT TAATTTAAAA CGTGAGTTTT GATCCTTTTT GTGGTTTGTT AGAGCGCAGA AACTCTGTAG AGTGGCGATA CAGCGGTCGG ACCGAACTGA AAGGCGGACA CCAGGGGGAA CGTCGATTTT GCCTTTTTAC TCCCCTGATT CAGACCAAGT GGATCTAGGT CGTTCCACTG TTCTGCGCGT TGCCGGATCA TACCAAATAC CACCGCCTAC AGTCGTGTCT GCTGAACGGG GATACCTACA GGTATCCGGT ACGCCTGGTA TGTGATGCTC GGTTCCTGGC CTGTGGATAA TTACTCATAT GAAGATCCTT AGCGTCAGAC AATCTGCTGC AGAGCTACCA TGTTCTTCTA ATACCTCGCT TACCGGGTTG GGGTTCGTGC GCGTGAGCTA AAGCGGCAGG TCTTTATAGT GTCAGGGGGG CTTTTGCTGG CCGTATTACC ATACTTTAGA TTTGATAATC CCCGTAGAAA TTGCAAACAA ACTCTTTTTC GTGTAGCCGT CTGCTAATCC GACTCAAGAC ACACAGCCCA TGAGAAAGCG GTCGGAACAG CCTGTCGGGT CGGAGCCTAT CCTTTTGCTC GCCATGCAT TTGATTTAAA TCATGACCAA AGATCAAAGG AAAAACCACC CGAAGGTAAC AGTTAGGCCA TGTTACCAGT GATAGTTACC GCTTGGAGCG CCACGCTTCC GAGAGCGCAC TTCGCCACCT GGAAAAACGC ACATGTTCTT

Enzyme AatII Acc113I Acc16I AccB1I AccB7I AccBSI AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AspI AtsI AvaI AviII BalI BamHI BanI BanII BanIII BbiII BbsI Bbv12I Bbv16II BcgI BcoI BglI BglII BpiI BpmI Bpu14I BpuAI Bsa29I BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI BscI Bse118I Bse21I Bse8I BseCI BsePI BseRI BsgI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiWI

Cuts 5 1 1 5 1 2 4 9 1 2 1 2 6 2 3 6 1 1 2 4 2 2 1 2 6 1 1 6 1 1 1 5 5 1 9 1 6 1 2 6 5 1 1 5 1 1 1 4 1 9 2 2 6 7 1 6 1 1 1 1 6 1 6 1 5 6 6 2 1

Positions 125 178 261 447 2552 3902 4160 465 691 2188 3199 4430 2495 3099 3544 629 2938 3423 3434 122 175 258 444 905 1556 2501 2549 3843 596 3010 5271 2043 1272 4383 620 712 1040 3715 3800 4961 3711 4957 963 1368 4862 613 687 804 859 1039 2332 1903 596 3711 4957 629 2938 3423 3434 7 4208 7 4208 3783 1272 4383 620 712 1040 3715 3800 4961 732 732 613 687 804 859 1039 2332 4160 2141 2037 465 691 2188 3199 4430 620 712 1040 2226 3169 1403 122 175 258 444 905 1556 2501 2549 3843 2489 620 712 1040 3715 3800 4961 2489 682 3867 613 687 804 859 1039 2332 90 212 283 2623 4265 609 2489 716 1011 2211 2574 4298 627 2489 1403 340 726 2523 3240 2786 122 175 258 444 905 1556 2501 2549 3843 2079 4316 2874 2973 706 2048 2771 3865 4014 4937 600 1544 1592 2043 4087 4918 5065 1403 701 1109 2043 2491 3135 4298 1903 2786 1403 761 833 1092 1212 1860 2076 2415 1608 706 2048 2771 3865 4014 4937 2786 465 691 2188 3199 4430 706 2048 2771 3865 4014 4937 620 712 1040 3715 3800 4961 3719 5103 1449

Sequence gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg cc/tnagg agc/gct g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c gacn/nngtc gacn/nngtc c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c at/cgat gr/cgyc gaagac gwgcw/c gaagac cgannnnnntgc c/ycgrg gccnnnn/nggc a/gatct gaagac ctggag tt/cgaa gaagac at/cgat yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw at/cgat r/ccggy cc/tnagg gatnn/nnatc at/cgat g/cgcgc gaggag gtgcag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg c/gtacg

BsmBI BsmI BsoBI Bsp106I Bsp119I Bsp1407I Bsp143II Bsp19I BspCI BspDI BspHI BspLU11I BspMI BspXI BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssHII BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI Bsu15I Bsu36I CciNI Cfr10I Cfr9I CfrI ClaI Csp45I CvnI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco81I

1 2 6 1 1 1 5 3 1 1 2 1 3 1 2 1 2 6 1 6 1 2 4 1 2 4 1 5 1 6 1 12 1 11 1 11 2 1 1 1 6 3 9 1 1 1 4 2 2 2 4 9 2 5 2 5 3 2 2 1 4 5 1 2 1 2 5 5 1

1279 2874 2973 613 687 804 859 1039 2332 1403 627 2756 598 2720 3085 3093 5031 360 2054 2405 4014 1403 3543 4551 5271 1118 1489 1795 1403 3099 3544 2786 4147 4329 701 1109 2043 2491 3135 4298 2756 701 1109 2043 2491 3135 4298 761 3719 5103 360 1056 2054 2405 627 3099 3544 360 2054 2405 2720 2700 598 2720 3085 3093 5031 2037 706 2048 2771 3865 4014 4937 2700 747 874 955 1369 1486 1729 1833 2610 3017 4137 4815 5006 340 585 609 1022 2037 2669 3736 3753 4521 4533 4619 4630 2205 585 609 1022 2037 2669 3736 3753 4521 4533 4619 4630 703 2768 1403 1903 2768 701 1109 2043 2491 3135 4298 687 804 859 703 899 1031 1169 1722 2139 2580 2768 3990 1403 627 1903 2826 3805 4497 4516 1077 2279 2652 3243 3287 5169 360 2054 2405 2720 703 899 1031 1169 1722 2139 2580 2768 3990 703 2768 1326 1356 1683 1882 3594 1272 4383 1326 1356 1683 1882 3594 618 710 1038 1272 4383 703 2768 340 360 1056 2054 2405 620 712 1040 2226 3169 3902 2079 4316 596 703 2768 1221 2121 2544 3701 4749 465 691 2188 3199 4430 1903

cgtctc gaatgc c/ycgrg at/cgat tt/cgaa t/gtaca rgcgc/y c/catgg cgat/cg at/cgat t/catga a/catgt acctgc at/cgat gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy g/cgcgc ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg at/cgat cc/tnagg gc/ggccgc r/ccggy c/ccggg y/ggccr at/cgat tt/cgaa cc/tnagg ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc cc/tnagg

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Vimentin
Eco88I Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I ErhI Esp1396I Esp3I FauNDI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I Ksp632I LspI MamI MfeI MflI MluI MluNI Mph1103I MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII 6 1 3 6 2 1 1 4 1 4 1 1 1 5 1 5 5 9 3 3 1 1 9 5 1 1 1 11 1 1 1 3 1 8 9 3 1 2 3 3 1 3 3 1 1 1 3 613 687 804 859 1039 2332 2700 618 710 1038 669 1084 1171 1489 1531 1564 1077 2279 2700 629 360 1056 2054 2405 5328 360 1056 2054 2405 2495 1279 234 620 712 1040 2226 3169 4160 716 1011 2211 2574 4298 598 2720 3085 3093 5031 122 175 258 444 905 1556 2501 2549 3843 1266 2595 2887 1266 2595 2887 622 2887 122 175 258 444 905 1556 2501 2549 3843 1326 1356 1683 1882 3594 627 2786 2874 585 609 1022 2037 2669 3736 3753 4521 4533 4619 4630 3010 2141 5328 701 1109 3135 2141 365 1775 2021 2056 2404 3612 3971 4130 122 175 258 444 905 1556 2501 2549 3843 3747 4688 4933 2874 2874 2973 703 1111 3137 360 2054 2405 234 701 1109 3135 701 1109 3135 591 2768 5328 3747 4688 4933 c/ycgrg g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg cgtctc ca/tatg grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc ctcttc tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg NspI NspV PaeR7I Pfl23II PflMI PinAI Ple19I Ppu10I PpuMI PshAI PshBI Psp124BI Psp1406I Psp5II PspAI PspALI PspEI PspLI PstI PstNHI PvuI RcaI SacI SbfI ScaI SexAI SfcI Sfr274I SfuI SmaI SnaBI SplI SrfI Sse8387I SspBI SspI SstI StyI SunI Tth111I Van91I VneI VspI XbaI XcmI XhoI XhoII XmaI XmaIII XmnI Zsp2I 2 1 2 1 1 1 1 1 1 1 2 3 2 1 3 3 1 1 5 1 1 2 3 3 1 1 12 2 1 3 1 1 1 3 1 2 3 4 1 1 1 2 2 1 4 2 11 3 2 1 1 1396 5275 627 613 1039 1449 2495 2043 4014 5324 1077 1451 7 4208 620 712 1040 3781 4154 1077 687 804 859 689 806 861 2700 1449 878 959 1373 1490 1733 591 4014 3543 4551 620 712 1040 878 1490 1733 3902 643 747 874 955 1369 1486 1729 1833 2610 3017 4137 4815 5006 613 1039 627 689 806 861 340 1449 689 878 1490 1733 2756 3448 3578 620 712 1040 360 1056 2054 2405 1449 732 2495 3711 4957 7 4208 2778 945 1650 1717 2742 613 1039 585 609 1022 2037 2669 3736 3753 4521 4533 4619 4630 687 804 859 703 2768 3783 5328 rcatg/y tt/cgaa c/tcgag c/gtacg ccannnn/ntgg a/ccggt cgat/cg a/tgcat rg/gwccy gacnn/nngtc at/taat gagct/c aa/cgtt rg/gwccy c/ccggg ccc/ggg g/gtnacc c/gtacg ctgca/g g/ctagc cgat/cg t/catga gagct/c cctgca/gg agt/act a/ccwggt c/tryag c/tcgag tt/cgaa ccc/ggg tac/gta c/gtacg gccc/gggc cctgca/gg t/gtaca aat/att gagct/c c/cwwgg c/gtacg gacn/nngtc ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy c/ccggg c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: AatI, Acc65I, AccI, AccIII, AclNI, AflII, ApaI, AscI, Asp718I, AvrII, BbeI, BbrPI, BbuI, BclI, BfrI, BlnI, BlpI, Bpu1102I, BseAI, BsiMI, Bsp120I, Bsp13I, Bsp1720I, Bsp68I, BspEI, BspTI, Bst1107I, Bst98I, CelII, Cfr42I, CpoI, CspI, Eco147I, Eco32I, Eco72I, EcoRV, EheI, FbaI, FseI, KasI, Kpn2I, KpnI, Ksp22I, KspI, MroI, MspCI, NarI, NruI, PacI, PaeI, PmaCI, Pme55I, PmeI, PmlI, PspOMI, PvuII, RsrII, SacII, SalI, SapI, SfiI, Sfr303I, SgfI, SgrAI, SmiI, SpeI, SphI, SseBI, SstII, StuI, SwaI, Vha464I

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010