Alleleustrious pmWasabi-Vinculin

Source Catalog Number: Introduction All ustrious pmTFP1-Vinculin is a mammalian expression vector that expresses mTFP1 fused to the N-terminus of a human vinculin (VCL) protein. This product can be a great tool for your research involving focal adhesions. All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is believed to function as one of several interacting proteins anchoring F-actin to the membrane. There are two transcription variant of this gene, variant 2 as in this product lacks a 3' coding exon. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment.
ele ele

ABP-FP-WVIN

Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Wasabi........................................613-1320 Linker......1321-1389 Vinculin...1390-4590 SV40 PolyA Signal.....................4742-4792 bla Promoter...............................5331-5435 Ampicillin Resistance Gene......5420-6280 pUC Origin..................................6429-7071 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C SV40 Poly A BLA AMPR Wasabi pUC Ori PCMV

pmWasabiVinculin 7153bp
Vinculin

Linker

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Critchley DR (2005). "Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion" Biochem. Soc. Trans. 32 (Pt 5): 8316. Hazan RB, Kang L, Roe S, et al. (1998). "Vinculin is associated with the E-cadherin adhesion complex". J. Biol. Chem. 272 (51): 3244853.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Vinculin
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGTCGCCA ATGAAGATCA GAGGGCGAGG GCCCCCCTGC TTCACCAAGT TCTTGGGAGC TCCATGGAGG AACGGCCCCG GTGCGCGACG CACCACCGCG TATCACTTTG ACCGTTTACG TCCGGACTCA AACGGCCGCA CAGCAGATCT CCTGACCTCA GGAAAAGAGA TTTATTAAGG GACCCTTACT GGAACATCAG TGCAAAGGAA GTCACTTACA AGACAGCAGG GTGAAAGAGT TCAAAAAACC AGTGCTGAAA TGGGCCAGCA AACCAGGCCA GCCATCAGAC CGCAGGGAGA CTCCGTGCCA CAGGGTCTGG ACCAACTCAA CCAAATGGTG ATAGCAGAAT ATATCTGCTC GAGGCTCGAG AACCGGGCTG GAGCAAGCAC GCCATCCGGG CGGCAAGATC CTGGCTGCCA GACTCCTTAA GTTTTCAGCG GATGCGCCTA GGAAAGCTTG GTGGAAGGCA GCTGCTCGTA ATGAAGAACC GATACCAAAT AAGGTAGCTA CGTCGGGCCA AAGTTCCGTG GTGATGGATG CTGGACTCAG CAGGAGCCTG CTTGCTCCTC CCAGAGGAAA ATGATGATGG GACATCATTG AGAGGGGGCA TCAGATGAGG AGAACCAACC CTGTCCACAG TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA CCATGGTGAG AGCTGAAGAT GCAAGCCCTA CCTTCTCCTA ACCCCGACGA GCACCATGAC AGGACTCCTT TGATGCAGAA GCGTGCTGAA TTGACTTCAA TGGACCACCG AGATCGCCGT GATCTCGAGC TGCCAGTGTT CCCACCTGGT CCGCGCCCGT CTGTTCAAAC TTGAGAATGC CAGTGCCTGC ACCTGCTCCT TTTTGGAATA CAAAGAATCT AGCTCACTCA TGCTGCCAGT AAGGCATAGA TTAATGAGAT AGGACACTGA AAGGTTGGCT AGATCTTAGA TTCTGGGAAC GAGGACAAGG ATGTGCTCAC AGCAGAGCAT GACCGGAAGG TATGTGATGA TGACTTCTAA CCTTGGCCAA TGGCCAACAG AGCGGTGGAT GGCTTGTGGC TTCTCGCCAA GAGGGGAAGG AGGATCTAAA ATACCACAAC ACAGGGAAGA GTGCTACGGC TTCAGGCCTC TCTTACTTAG AGTGGATCGA CTCTGTTGGA TGGCCAACAT ACCGGATCCT AGGCTGTGAA CAAAAGCTGT GATATCGGAT ACTTCCCGCC CCAAACCACC AGGATGAAGA CTGCCAGACA CAGCAGCCAA GTGGTACCAA TGACTCGGTT TCTTACAGGT TGAAGGCCAC TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGGGCGAG GGAGGGCAAC CGACGGCACC CGACATTCTG CATCCCCAAC CTTCGAGGAC CATCTACGAG GGAGACCACC GGGCGACGTC GACCATCTAC CATCGAGATC GGCCCGCAAC TCAAGCTTCG TCATACGCGC GATAATGCAC GGCCGCCGTG CACTGAGGAT TTGCACCAAG TCGAGATTAT TACCTTCGAT TCTTACAGTG TGGGCCAGGA CCAGGAGCAC TCTCATTTCA GGAAGCTTTA AATTCGTGTG AGCCATGAAG CCGTGACCCT TGAAGCTGGA TTGCAAAATG ATCCTCACCG AGCAAAAGTG TGCAAAGAAG AGAAGAGCAG TCCTAAAGAA ATTAGCAGAT ACAGGTGGCC CAGACCGGCC TGATAATCCC CGAAGGGCAT GTGTGACCGA GGAGAGTCCT AGCTCGGATG TCCCATCAAG GGTATTTGAT CGAGAAGGCG AGTGAAGACG GAACCCTGGA TAATGTTGAA TGCTTCAGAA TCAGCCTCAG GCTGGTGGCT AGCTGCCTCT GGCTGGAAAC CCTGGGAGCT GCCTCCACCA TCTGCCTGAA GTTCCCTGAG GCTCCATGAT GCGCATGGCT GCGGGCACTC GGCCAAGGAG ATGTGAGCGA CATGCTGGGC ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC GAGACCACAA GTGAATGGCC AACACCATCA ACCACCGCGT TACTTCAAGC AAGGGCATCG ATACACCTCA GGCTGGGACG AAGATGAAGC AGGGCCAAGA CTGAACCACG TCCACCGACG AATTCGGGAA ACGATCGAGA GAGGAGGGCG CAGGCGGCCG CAGATTTTGA CTTGTCCAGG CTAATTGATG GAGGCTGAGG GCAGAGGTGG ATGACTAAGA CGAGTGATGT GCTATGAAGA AAAAATCGCA TTACAACTCA AGAGCATTGG AGTGCCTCCC AAAGTTGGTG CTAGGGCAGA GTGGCCATGC GAAAATGCAG ATCGATGCTG ATTCGAGGTG AGAGATGACA CTACGAAGAC ACGGCCCTGC AAAGCAGCTG ACAGTGGATG CGTCTGGCTA GTGGACCAGC CAGGCACGAG CAGGAGGCCA CTGTTGGCAG GAGAGGGCAG GCTGCGGTTG GCCCGAGAAC AATCAAGCTG AAAATGACAG GAAGCAATTA ATGCTGGTTG AAGAGGGAGG GATGAATTGA ATTTCCGACC GTGGCCAAGG GACCTTGAAC GGTGAGGTCC CAGAAGGCCG GAAGCTCGCA CTGCTGATGG ATTCAGTGTG GTTGCCAAGC ATCCCAACCA CGGACCAACA AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC TGGGCGTAAT ACGCCTTCGT ACCTGGAGGT TCAGTTACGG AGTCCTTCCC TGAAGGTGAA AGGGCGAGAA CCTCCACCGA TGCTGCTGGA AGGCGGTGAA ACAAGGACTA GCATGGACGA ACTCGAGAAC GCATCCTGGA AGGTGGACGG TCAGCAACCT AGAGAGATAT CAGCTCAGAT GGTCAAGGGG TCCGTAAAAT TGGAGACTAT TGGCCAAGAT TGGTGAACTC TTTTTGTAAC ATTTTACTGT CCTCTTGGGA CCTCCATAGA CAGGGGATGC AACTCTGTGC TGACTGATCA AGAAAGCTCA CTCGCAAGCT CTCAGAACTG CTTTGGCTGA TTCTACGTTC AGGGGAAAGG AGAACCTGCA TACACCTTGA ACCGTGGAGT ATGTTATGAT TGACAGCCCA CACTTGCATC TGACTCAGGA TGGCAGCCAC CTAACTTTGA GTACTGCTAA TCACACCCCA CTTATGAACA GGCTGGTGGA AAAAAGACCT CTGGGGCAAC TGGAGAATTC GCAAAACCAT CTGGACTGCA TCAGAGAAGC AACTCCGACT CTCCACCTAG GGGAGGTGAT AATGGTCCAG CTGAGATGTC CCAAGGACAT AGTGCACAGA TAAGCACCCA TCAGTGATGA TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CAAGCCCGAC GATCGAGGGC GAAGGAGGGA CAACAGGGCC CGAGGGCTAC GTCCGACATC CTTCCCCCCC GAGGATGTAC GGGCGGCGGC GCTGCCCGAC CAACAAGGTG GCTGTACAAG AAAGCTTGAA GCCGGTGGCA CAAAGCCATT CGTCCGGGTT GCCACCAGCA GCTTCAGTCA CATCCTCTCT TATTAGAGTT GGAAGATTTG GATTGACGAG GATGAACACC AACTAAAAAC AGAAAAAATG TGAAGATGCC CTCCAAACTG TGGTGAGCAG AGGCAAAGAA AGTGGCTGAC GCAGGTATCT GGAAGCCATG GCTTGCAGAT AGCTCGGAAA CCTTGGGGAA AGATTCTCCA GACCAAAACC GGGCAAGATT CGGTCAGGCT GGGGCCTTAT GCTGGCTGAC TCAGCTCCAA AGTGTCAGAT GGCGCCTCCT AAACCATTCA TAAATCAACA GGTGGTCTCG TTTTGAGACC CGAAGCCATT GGACAAGTGC CAGTATTGCT CGAGGATCCC CTCCCCGATG AAAGAGCTTC CTTCCAACCT AACAGATGAG GCCTCCACCA TAACCAGCCA CAAGGGCAAT TCGGCTGGTA CGCCAAGGCC TAAACGGATT GCTCAAAATC GGAGTCTGAG

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Vinculin
4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 5281 5341 5401 5461 5521 5581 5641 5701 5761 5821 5881 5941 6001 6061 6121 6181 6241 6301 6361 6421 6481 6541 6601 6661 6721 6781 6841 6901 6961 7021 7081 7141 4441 CAGGCCACAG AGATGCTGGT TCACAATGCC CAGAACCTCA TGCAGTCTGT GAAGGAGACT GTGCGGGAAG CTGAAGCTGC TTCAATCAAA ATTCGAACAG ATGCTGGATT TACACTGCGC TGGGTTAGAA AGACTCCCTG GTACCAGTAG GCGGCCGCGA CTCTAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCATG CAT

Enzyme AatI AatII Acc113I Acc16I Acc65I AccB1I AccB7I AccBSI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII

Cuts 3 5 1 1 2 7 3 2 1 7 8 1 2 2 1 9 3 6 6 2 1 3 7 3 3 3 2 1 9 6 1

Positions 3376 4011 4258 125 178 261 447 1109 5726 5984 4213 4580 465 745 3231 4213 4580 5023 6254 1052 2234 2587 4923 5368 1321 1350 1808 3705 4529 4762 5247 5258 122 175 258 444 1058 1106 3232 5667 596 4834 7095 600 2427 6207 1341 1934 1949 2477 3102 4306 5539 5624 6785 4302 5535 6781 1696 2466 2805 3633 4543 6686 889 1334 1362 1700 2765 3392 3089 3899 596 4302 5535 6781 1350 1808 3705 4529 4762 5247 5258 7 2111 6032 7 2111 6032 3368 3810 5607 4213 4580 6207 1341 1934 1949 2477 3102 4306 5539 5624 6785 889 1334 1362 1700 2765 3392 5984

Sequence agg/cct gacgt/c agt/act tgc/gca g/gtacc g/gyrcc ccannnn/ntgg gagcgg t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg cc/tnagg agc/gct g/tgcac r/aatty at/taat at/taat gaann/nnttc g/gtacc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca

AvrII BalI BamHI BanI BanII BanIII BbeI BbiII BbsI BbuI Bbv12I Bbv16II BcgI BclI BcoI BglI BglII BlnI BlpI BpiI BpmI Bpu1102I Bpu14I BpuAI Bsa29I BsaAI BsaBI BsaHI BsaI BsaMI BsaOI

1 9 4 7 4 2 1 8 2 1 9 2 1 1 6 9 5 1 1 2 5 1 2 2 2 3 1 8 5 4 9

4006 698 1903 2434 2776 2788 2833 3613 3874 4282 2419 3674 3714 3857 465 745 3231 4213 4580 5023 6254 783 1341 1934 4993 2552 3497 3235 122 175 258 444 1058 1106 3232 5667 2740 3389 1392 1341 1934 1949 2477 3102 4306 5539 5624 6785 2740 3389 5691 2385 889 1334 1362 1700 2765 3392 90 212 283 1180 1531 3000 3052 3325 6089 1330 1445 2291 2727 3006 4006 2447 2740 3389 768 1131 1431 2761 6122 2447 1348 4533 2740 3389 2552 3497 340 1080 5064 4610 122 175 258 444 1058 1106 3232 5667 636 1047 3419 3480 6140 1640 3373 4698 4797 605 1386 1415 1538 3320 4595 5689 5838 6761

c/ctagg tgg/cca g/gatcc g/gyrcc grgcy/c at/cgat ggcgc/c gr/cgyc gaagac gcatg/c gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc a/gatct c/ctagg gc/tnagc gaagac ctggag gc/tnagc tt/cgaa gaagac at/cgat yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Vinculin
BsaWI BscI Bse118I Bse21I Bse8I BseAI BseCI BseRI BsgI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiMI BsmI BsoBI Bsp106I Bsp119I Bsp13I Bsp1407I Bsp143II Bsp1720I Bsp19I BspCI BspDI BspEI BspHI BspLU11I BspMI BspXI BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I 8 2 7 2 1 1 2 5 2 9 1 7 9 9 4 1 4 6 2 2 1 2 5 1 3 2 2 1 2 1 3 2 2 1 5 7 2 7 4 11 2 2 8 1 5 4 9 1 8 1 19 600 1321 2427 2592 3671 5911 6742 6889 2552 3497 600 1048 1431 2427 2844 4959 6122 3089 3899 4610 1321 2552 3497 633 972 1476 3970 4434 1533 2332 605 1386 1415 1538 3320 4595 5689 5838 6761 4610 465 745 3231 4213 4580 5023 6254 605 1386 1415 1538 3320 4595 5689 5838 6761 1341 1934 1949 2477 3102 4306 5539 5624 6785 1473 3100 5543 6927 1321 1640 3373 4698 4797 889 1334 1362 1700 2765 3392 2552 3497 1348 4533 1321 1313 2859 598 3235 4909 4917 6855 2447 360 611 962 1415 5838 2552 3497 1321 5367 6375 7095 1756 2456 2809 2552 3497 4923 5368 4610 2543 4141 4151 5971 6153 600 1048 1431 2427 2844 4959 6122 1313 2859 600 1048 1431 2427 2844 4959 6122 1473 3100 5543 6927 360 611 962 2049 2691 2771 3875 4006 4241 4253 4283 1348 4533 4923 5368 360 611 962 1277 1517 2789 2922 3227 1257 598 3235 4909 4917 6855 2419 3674 3714 3857 605 1386 1415 1538 3320 4595 5689 5838 6761 1257 1167 2087 2797 2806 4841 5961 6639 6830 340 585 1226 1330 1445 2291 2419 2577 2727 3006 3132 3674 3714 3857 5560 5577 6345 6357 6443 6454 762 2784 4164 4404 585 1226 1330 1445 2291 2419 2577 2727 3006 3132 3674 3714 3857 5560 5577 6345 6357 6443 6454 1383 1535 3317 4592 2552 3497 3089 3899 4592 2447 600 1048 1431 2427 2844 4959 6122 696 1137 1383 1520 1535 1901 2432 2774 2786 2831 2846 2957 3317 3611 3872 4280 4592 5814 2552 3497 1348 4533 3089 3899 2069 4650 5629 6321 6340 836 2992 3996 1209 1458 1953 5067 2926 5111 6993 360 611 962 1277 1517 2789 2922 3227 696 1137 1383 1520 1535 1901 2432 2774 2786 2831 2846 2957 3317 3611 3872 4280 4592 5814 1383 1535 3317 4592 1604 2192 2607 3261 4041 5418 6207 1604 2192 2607 3261 4041 5418 1339 1932 6207 w/ccggw at/cgat r/ccggy cc/tnagg gatnn/nnatc t/ccgga at/cgat gaggag gtgcag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg t/ccgga gaatgc c/ycgrg at/cgat tt/cgaa t/ccgga t/gtaca rgcgc/y gc/tnagc c/catgg cgat/cg at/cgat t/ccgga t/catga a/catgt acctgc at/cgat gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy EclXI Eco105I Eco130I Eco147I Eco24I Eco255I Eco31I Eco32I Eco47III Eco52I Eco57I Eco64I Eco81I Eco88I Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I EheI ErhI Esp1396I FauNDI FbaI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I KasI Kpn2I KpnI Ksp22I Ksp632I LspI MamI MfeI MflI 4 1 11 3 4 1 5 1 1 4 10 7 2 6 1 2 1 3 1 2 1 11 1 1 11 3 1 1 4 1 5 5 8 2 2 5 1 8 1 1 2 1 6 2 1 1 19 1383 1535 3317 4592 340 360 611 962 2049 2691 2771 3875 4006 4241 4253 4283 3376 4011 4258 783 1341 1934 4993 5726 636 1047 3419 3480 6140 3853 596 1383 1535 3317 4592 678 1101 1677 2182 2632 3568 3991 4516 5525 6573 465 745 3231 4213 4580 5023 6254 3089 3899 889 1334 1362 1700 2765 3392 1257 1339 1932 4333 836 2992 3996 1257 1350 3705 3853 360 611 962 2049 2691 2771 3875 4006 4241 4253 4283 7152 3233 360 611 962 2049 2691 2771 3875 4006 4241 4253 4283 1052 2234 2587 234 2385 783 1341 1934 4993 5984 768 1131 1431 2761 6122 598 3235 4909 4917 6855 122 175 258 444 1058 1106 3232 5667 1152 4711 1152 4711 1343 1372 1648 2063 3304 4711 122 175 258 444 1058 1106 3232 5667 3231 1321 4217 4584 2385 1604 2192 2607 3261 4041 5418 1348 4533 4610 4698 585 1226 1330 1445 2291 2419 2577 2727 3006 3132 3674 3714 3857 5560 5577 6345 6357 6443 6454 4834 698 1903 2434 2776 2788 2833 3613 3874 4282 7152 1321 4959 698 1903 2434 2776 2788 2833 3613 3874 4282 365 613 961 1393 2892 4450 5436 5795 5954 122 175 258 444 1058 1106 3232 5667 2857 2892 3039 3051 5571 6512 6757 4698 1640 3373 4698 4797 4961 3232 360 611 962 234 4959 4959 591 4592 7152 2857 2892 3039 3051 5571 6512 6757 1392 7099 1348 4533 1392 1334 1362 1700 2765 1052 2234 2587 600 2427 1415 5838 3376 4011 4258 7148 3996 7 2111 6032 1341 1934 5605 5978 3996 1257 2801 2810 c/ggccg tac/gta c/cwwgg agg/cct grgcy/c agt/act ggtctc gat/atc agc/gct c/ggccg ctgaag g/gyrcc cc/tnagg c/ycgrg g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc gat/atc c/cwwgg atgca/t ggc/gcc c/cwwgg ccannnn/ntgg ca/tatg t/gatca grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc g/gcgcc t/ccgga ggtac/c t/gatca ctcttc tt/cgaa gatnn/nnatc c/aattg r/gatcy

MluI MluNI Mph1103I MroI MroNI MscI MslI

1 9 1 1 1 9 9 8 7 1 4 1 1 3 1 1 1 1 1 1 7 2 2 1 4 3 2 2 3 1 1 3 2 2 1 1 2

a/cgcgt tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc gg/cgcc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa gcatg/c c/tcgag ccannnn/ntgg a/ccggt cgat/cg agg/cct a/tgcat rg/gwccy at/taat gagct/c aa/cgtt rg/gwccy g/gtnacc ctgca/g

BstXI BstYI

4 19

ccannnnn/ntgg r/gatcy

Msp17I MspA1I MunI Mva1269I NaeI NarI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI NspV PaeI PaeR7I PflMI PinAI Ple19I Pme55I Ppu10I PpuMI PshBI Psp124BI Psp1406I Psp5II PspEI PstI

BstZI Bsu15I Bsu36I CciNI CelII Cfr10I CfrI

4 2 2 1 1 7 18

c/ggccg at/cgat cc/tnagg gc/ggccgc gc/tnagc r/ccggy y/ggccr

ClaI Csp45I CvnI DraI DraII DraIII DrdI DsaI EaeI

2 2 2 5 3 4 3 8 18

at/cgat tt/cgaa cc/tnagg ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr

EagI Eam1104I Eam1105I EarI Ecl136II EclHKI

4 6 1 6 2 1

c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Vinculin
PstNHI PvuI PvuII RcaI SacI SapI ScaI SexAI SfcI Sfr274I SfuI SgrAI SnaBI SphI SseBI SspBI 1 2 3 2 2 1 1 1 8 4 2 1 1 1 3 2 591 1415 2857 5367 1341 2608 5726 1454 1167 6639 1334 1348 2427 340 1392 3376 1313 5838 3039 3051 6375 1934 g/ctagc cgat/cg cag/ctg t/catga gagct/c gctcttc agt/act a/ccwggt c/tryag c/tcgag tt/cgaa cr/ccggyg tac/gta gcatg/c agg/cct t/gtaca SspI SstI StuI StyI Van91I VneI VspI XbaI XcmI XhoI XhoII 2 2 3 11 3 3 3 1 6 4 19 5272 5402 1341 1934 3376 4011 4258 360 611 962 2049 2691 2771 3875 4006 4241 4253 4283 1052 2234 2587 4302 5535 6781 7 2111 6032 4602 1299 2826 3210 3552 3774 4452 1334 1362 1700 2765 585 1226 1330 1445 2291 2419 2577 2727 3006 3132 3674 3714 3857 5560 5577 6345 6357 6443 6454 1383 1535 3317 4592 3368 3810 5607 7152 aat/att gagct/c agg/cct c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy

2087 2797 2806 4841 5961 6830 1362 1700 2765 4533

4011 4258 2859

XmaIII XmnI Zsp2I

4 3 1

c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: AccI, AclNI, AflII, ApaI, AscI, AspI, AtsI, BbrPI, BfrI, BsePI, BsiWI, BsmBI, Bsp120I, Bsp68I, BspTI, BssHII, Bst1107I, Bst98I, Cfr42I, Cfr9I, CpoI, CspI, Eco72I, Esp3I, FseI, KspI, MspCI, NruI, PacI, Pfl23II, PmaCI, PmeI, PmlI, PshAI, PspAI, PspALI, PspLI, PspOMI, RsrII, SacII, SalI, SbfI, SfiI, Sfr303I, SgfI, SmaI, SmiI, SpeI, SplI, SrfI, Sse8387I, SstII, SunI, SwaI, Tth111I, Vha464I, XmaI

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
ele

Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010