Tugas Genetika Sel dan Molekuler

“RNA interference”

Nama : Giano Excelsis Pangemanan

NIM : 22/501739/PBI/01863

Candidatus kuenenia stuttgartiensis(norVW)

ACCESSION CT573071 REGION: 865238..866413

1 ATGAAACCGA TACCACTAAA AGAAGGAATT TATTGGGTTG GAGAAGTTGA CTGGGATTTA

61 AGAAATTTCC ATGGTTATTC AACCAACCGG GGGTCTACAT ACAATTCCTA TTTGGTAATT
121 GATGAAAAAA TAACATTAAT TGATGGCGTC AGGCCGTATC TCTTCGACAA AATGATGCAG
181 CGCCTTTCCT CTCTTATTGA TCCCGCAAGT ATTCAGTATG TTATTGCAAA CCACGTCGAA
241 ATGGACCATT CCGGCAGTTT ACCGCTTATT ATGGATATTG CAAAAAATGC CACTATTGTA
301 ACCTCCCTTA ACGGACAGAA AGGGCTGCTG GCGCATTATA AGGGGAACGA CTGGAATATG
361 AAGGTGGTGC AAACGGATGA GGAAGTCAAT ATTGGCAAAA GGACCTTCAG GTTTTTTCTG
421 ACCCCAATGG TTCATTGGCC GGATAACATG GCAAGTTATC TGGTTGAGGA TAAAATCCTT
481 TTCTCGAATG ACGCCTTTGG ACAGCATATC GCTTCTCATG AAAGATTTGA TGATGAACTT
541 TACCTTGGAG CCGTTATTGA AGAGGCGAAA AAATACTATG CGAATATCGT CCTGCCCTAT
601 TCCATGCTAG TGAAAAAAAC CATTGAAAAA CTTTCTCATA TCCCGATTGA CATGATTGCT
661 CCAGGTCATG GAATTGTATG GCGCTCACAT ATAAAAAATA TTATAGATGA GTACAATAAA
721 TGGGCGGCCA ACCAGACAAA GAATAAATGC CTTATTATTT ATGATACCAT GTGGAAATCC
781 ACGGAAAAAA TTGCACATGC TTTACAAAAC GCCTTCAATC ACGGCGGTAT TAGTACAAAG
841 ATGATTAATC TCCGTTATAA TCACCTGTCG GATGTTGTTA CGGACGTATT AGACGCAAGA
901 TTTATTTGTG TGGGATCTCC TACATTAAAC AGTAATTTGC TTCCTACCGT GTCGGCATTT
961 CTCACTTATT TAAAAGGGCT TTCGCCGAAA GACAGGATTG GCCTGGCATT TGGTTCTTAT
1021 GGATGGGGAG GGCAGTCTGT TAGCCACGTG GAAGACGCAC TTAAGGAGTG TGGTTTTGAT
1081 TTGCTGGAAA GCATAAAAAT CCAATACATT CCCGACGAAA CGCAACTTCA GGAAATCTAT
1141 GAAAAAACAA AACAGGAAAT TGCAAAGAGA GGATAA

NosB cDNA : 5’ AAACCACGTCGAAATGGACCATT 5’

│││││││││││││││││││
Sense siRNA : 5’ ACCACGUCGAAAUGGACCA – dTdT 5’
│││││││││││││││││││
Antisense siRNA : 3’dTdT– UGGUGCAGCUUUACCUGGU 3’