Exón 1.

Análisis de la secuencia de nuestro gen SEC61B de Homo sapiens (1…82)

1 agtctcgcca gctgccggtc tttcgggggc tccgtaactt tctatccgtc cgcgtcagcg

61 ccttgccacc ctcatctcca atatggtatg gcggcccttc catgatcccc gcctctccca CDS 1
121 gaagccctga ctcctcctgc tttgcgccgt gcttttcctc tgtagctccc ttgcttcccc (83..85)

Intrón 1
181 cagcctcggg tgtgggtgtc taggccgggg ttctggggca ggcctgccgc gctcacccgt
241 ctgtctgctt gtctccctct acagcctggt ccgaccccca gtggcactaa cgtgggatcc
Exón 2.
(86…264) 301 tcagggcgct ctcccagcaa agcagtggcc gcccgggcgg cgggatccac tgtccggcag
361 aggtaaggaa ccctgcagtt cgttcgcttc cagactcgga gataggaccc agaacctcgc (265…362)
421 tgattctggg gtggagaccc tagcatgtga agattgacaa aggcaaaatg agcttctagt
481 gacgtggccg tgggagtagt taaaggcctt ttgggaggaa ggcgacattt tttttctcgt
CDS 2
541 tgctcagttt agggcactac tcttaaaaaa ggaaagttaa caaactggaa tagagtcaga
(265…362)
601 gataactttg agaaaaccga tgtcattaaa ctggtgtctc tggacctgag gtttgcactc
661 acatttccat ctggcggccc cataagcaat ctgtcctaca gataactcgt cctacacaaa
721 acttagtctc ttttcagctc agctctctca ctctcaatta tatctcctta cttccatatg
781 gcactgttgt acactcattt actcagagcc agaaacgtca gcgtcatctt ggatttttct
Intrón 841 tatgctcttt ctctctctag tcatatgcca gactttaaac tctgcttgaa agctttctca
2 901 taagctcttt ccttttccct ttctactgct ttgcatttgc tacttaaccc ttttcttcag
961 gctgtttgct ttccagtcca tcgttcgctc tgctgttact cttctgcgta gtttctgtta
(363… 1021 cttgttgctg aactaaaaaa aacaaacaaa caaaaaaaaa aaaccggggg gataccaaag
5617) 1081 ctcttagtgg tcatttcagc ctaacctttc tttttccttt tttttttttt gggatggaat
1141 ctcactctgt cgcccaggct ggagtgcagt ggcacgatct cggctcacta cagcctttgc
1201 ctccagagtt caagcaattc tcctgcctca gcctcctgag tagctgagac tgcaggcacg
1261 tgccaccaca cccagctaat tttttgtatt tttagtagag acggggtttc accatgttag
1321 ccaggatggt ctcgatctcc tgaccttgtg atcctccgtc tctgcctcca aaagtgctgg
1381 aattacaggc gtgagccact gcgcccggcc ttcagcctaa tctttctttg ctgtcctatg
1441 atccagcact cgtgaaccca ttactgtttt ctgaattcac aaactttctc cctcatctca
1501 aggcaaattt ctacttattt tccaagacta agctcagggg ttatttcctc ttgaggcctt
1561 ctctgtctgt aagcagcagt agctactagt tttccctttt gcccccatag cattttgttc
1621 atatcattat aagaccagtg atgtttccat atttatctcc ttcattagac tttaacagag
1681 aacctgctca agtcatttct atcccaggta cccaccataa ggcatgacac aataaataat
1741 gactgaagga aggaatcaag caaccaagtt gttttgggat ttttgaagga actagtgggg
1801 ttttatctag ggaggaaaaa atcttgaaca catgcatgga aacttctgaa ggatgtgaag
1861 agggatgtga cattttctgg ctgcaacagg taaatctaaa actgatgatt tgaacttaca
1921 tgaaatattt cacatcagaa caaagacatt ctaatagaac tggcagaaat gtaaaattat
1981 cctgaatgat tttgagttcc ctgtcattgg gatgttcatg aggagtgtat caggctgtgg
2041 tttaacttat atgggctttg agtgctgtcc ccttcccacc ctgggtttta gagatttttc
2101 gtgcaggaat ggaaaacatg tcctgtgggt gcggttcctt gggaatggtt ctgcttaatg
2161 agaattggta atactgtggc agggaagttt ggccatccga gaatttgtaa agaaataaaa
2221 gaaaaagcac atatttatca aatgtacata tgataatgaa attatttatt taatagtaaa
2281 aatgaaataa ctgatgcaca agtgaaatgg agaactgaaa catttgaaga cagacagatg
2341 cagtttttaa agtcgtgggt gttggtgtca tcttttagga ttcagtgtga tagagctagt
2401 gtaacaagaa agaccatggt tgtatgtatt gagagacctg acagtgtttc cttctagaat
2461 ggtagtgatt aagtatcatt tcatgagtat aatgtaataa gtattggttg tttaagaata
2521 tctttgcttt ataagtctga gaaaacattg gaagttaatg attcataata gtgctttgga
2581 ttatgttgtc tgtagagctg ccttctccat gcttgatgag gtgatagata ctaccttgga
2641 ggagaaatgt tattaaattt aagctcaggc cagagttggt gttcttgctc tcctaaatac
2701 aatataaaag gaacttgctg tgaggagcaa taatgcttta taaactgtaa aggtgtgaag
2761 tgccacatag tgttctagca ggattaatgg gaatcaggga tattaagatg gcggaatagc
2821 caccaatgta tagacatcaa ggtattgagg tctactacat gccaaatgct gtttcatctc
2881 cgaagatggg tctgtgaaca aggtaggcaa ggtttgtact catgaacctt atattccagt
2941 gtgggagatc aaacgcaagg aaaataagac aatgtaattt caggcaataa gggctatgaa
3001 gataaataaa gctgggtggg ggatagagag tggtggggat agggcaaagg taggtagttg
3061 ttttagatag ggtgttcaca gtaggtaata cctgagtaga tacctgaaag aagcaagaga
3121 gcaaatcaca caatggtttg gggacgaata ggcctcctag gcaaaaggag tgggtaagta
3181 tgaagattct gaggtcgatg aggttggctt tgaggagcaa cagaagcacc atgttagaac
 3241 caagtgtgta actggttagt tttgaaaacc attgtgttga ggtatagaag ggagagacag
3301 ttacacagtg gccagaaaga aagggtaaaa tagcccccaa atacaaattt gagagattca
3361 atgtgtttta ttaaccttta cctagctacc cctagggaat gggaagtatt ttgagtacac
3421 tttagtattt ctaggggctc tggttttcaa atactcttct atgttccatg cgccagcaga
3481 aataagaaat cttaagagaa gccagcctga ctcctctggg gctcctgtaa ttccaggaaa
3541 ctttgaagtc aggaactgga ggtgtgatat tgtggaaata gtaggaagtg catgcgtcca
3601 agtcctggct cagtccatga ctataatatg ttcacactgg gtttatcttc tagcttaaaa
3661 tccttttcaa tgaacaagga ggaagatttc agctgcatac cccacagatg gaatgacata
3721 tatctgtgtg tgtgaaacct tttctaaggg catgaatctt tctgtaaatc cctgtacagt
3781 tatgttttgc catttacaat cagttttccc ataggaatgg tcacacaatg aagttttaag
3841 ttcccaggct accacgtagt ccataattaa tgtgtctgaa atataatcct tgtagtacaa
3901 tagacccaaa tcatggtttg gcactgattg ctttcaaagt tctggcatga acacagctct
3961 tcctgttaga accaggactg cactctccca ctccgtcatg tagcagggca ggctccccgt
4021 gctgtagaca gtgcttcctg cattcagaat gtggagtaaa gaccgtggtt gggtaaagga
4081 ggcatgagga ccagaatggg gaggaaagca agaagagatg gtagggatag ggttttcact
4141 tctcttcttt tccgtatatg cccttccctt tttcaaaaat ccagaatcat cagcccacag
4201 atactggaat gagattttga gggagggtat actgggattt gatgctgaat ggtgcaggca
4261 gcaatgagat aggcttcccc tctcctcccc actaggactt ctgagttggt gatacatggt
4321 ctgaagaaaa ttgcttgtga cttctcacca cttgcccctt acccccatat tgtaccactc
4381 caacatgggg cacgatgagc agctggggtt ggggaagtac tgcagggaag ggtagttgct
4441 gggtggggtt ccccggtgac tgagggcttc tcccaaagag gagagaaagg attccttggg
4501 cctattcctt ggcagcagag tcctaagtgc tgctaggctg cttctgtctg ccagggctgc
4561 gtgtattaca tagtgtatct tacaagcctt gatccccttg tgagagacac aaggggtgtt
4621 ttggttgggt gttccctgcg ttttttttac attctacctc tctgtgcaag atctgtgatc
4681 actatattaa aatagtgaaa acacggccag gtgcggtggc ttgcacctgt aatcccagca
4741 ctttgggagg ccaaagcagg cagatcatga ggtcaggagt tcaagaccag cctggccagt
4801 atggtgaaac cctgtctcta ctaaaaatac aaaaattagc tgggcatggt ggcgcacgcc
4861 tgtaatccca gctaagcagg aggctgaggc agaagaatcg cttgaaccca ggaggcggag
4921 gttgcagtga gcagaaattg tactactgta ctccagcctg ggcaacagag cgaaactcca
4981 tctcaaaaaa aaaaaaaaaa aaaaaaaaaa caaactagtg acaacacata acaaagtaat
5041 tttttataaa gtgtatactc tatcttgggc cctggatata attaaagctt aaagtttttg
5101 tctgtttgca gtaaatatac taattttaaa atacatcttt taaagtagcc attttaagat
5161 cagcttgtat ggattcaggg cattttaaaa caggaattta ttatttggct tcatgaaaga
5221 tgcatgtgag gagaaagtga aatgctttgt ccacatgaca cagctggatg ttttgggcat
5281 gcagatgggc tccagggtca gagctaggtt cagccacctg ttggctgcag ggtcttagat
5341 attttgctta accttttctg aggccaattt gcctcattta taaaatggaa aaatcgtgac
5401 tgcttttcag ggttattgta tgcattggca tcaggtaggc acttagttca ttgtggttat
5461 catgatcaaa ggagtaaaag caggaattta ggtggattct agcaaagaaa gttttttttt
5521 tttgattcct taactgtgta ataatggtag catatgttat aatgctattc taataatttc
5581 cagaaaaggc catttgtaac attttcctct ctttcaggaa aaatgccagc tgtgggacaa
Exon 3.
5641 ggagtgcagg ccgcacaacc tcggcaggca ccggggggat gtggcgattc tacacagaag (5618…5719)
5701 attcacctgg gctcaaagtg taagtcttag gaacagtcct tgtgtttctg tccagtggca
5761 gcagagcagc agtggcagca ctctgtctct gtcaccagta gcgtttgcct tgatgcgatt
5821 tactgtacac aacagcctca tttgtgccag gcggactggg tttggtttgc ctgtttatca
5881 accgattagg cattgtaggg ccgggaatga catcttttca tggagtgagg gaaaggaaag CDS 3
5941 gaagttcttc atggagctat gtcaaaataa atgagaacag agagaactgg agggaaaact (5618… 5719)
6001 gcttaattag atgaaattgc tcttcatttg gtgatcttgc cccgtaccaa gaaataggag
6061 taatcatttg aataggaatt gggtgatttt tcaaagttct cctgaaggca taagctgata
Intrón 3 6121 ttactacaac ctgatggagg ttttcccatt aggctgtgtt accaggagtt ccttggcctc
6181 tttgcctttg gtgggtggtg agtagactca ggagggtgaa catgtaatac cactttccaa
(5720… 6241 gttcttaggc attggagtgc tcattaggtg atgggaaatg ggtgtccagg gacccctgtc
8055) 6301 cccatgcctt gctcagggtt tctggtgctg tgtggcagat cttatcccac agatttctgt
6361 taggttccag aacaccactg cctacaactg tttcctgact aaactcggtg gatgaggatt
6421 cgaaataata acagcaactc tgatttacat agcactttgt actttgcaaa gaactttcaa
6481 tgtctgttgt ctagtttgat ctgcccagca atccagtgag gtgagagatg ttaccatttt
6541 gtagatgagg acctcaggtt caaagaggca aagtgacatg ctcaaagaca cacaacaaga
6601 ggtggaactg gatattctga ttaagtccta tgctctttgc atattgtcca tctagttaaa
6661 ataaaaaaga caaaattgaa ttaatttgga ttagttttaa ttaatttgac cagcaccttg
6721 actttagttt acaaagtcac aaatttgtaa ctttacaaat ttgtaaagtt tttattaatt
6781 ttaattagtt tttataattt taattagttt taattaattt gaccagcacc ttgactttag
6841 tttacaaagt cacaaaagga tcacaaattt agtttataaa gccttccctg gaattcttgt
6901 tttctcttat attggagact tatatgataa tgtgagaata tatctaggaa gtaattcttg
6961 gggttgggga tatgtgaggt gggggtatcc tgactgccaa ccttttggtc tttggtggag
7021 gtagtttgag tatttcagag agaatttttt tttctccttt agcgacaggg ttttgctctg
7081 tcgcccaggc tggagtgcaa tggcatgatc atagctcatt gtagtcttga attcctgggc
7141 tcaagtgatc tttactccag gagacaaaat tggataaatt tggattaatt agtcctgagt
7201 agttgggact acaagtgcgc actaccatgc ctggctagtt ttttgtacaa gcagaatctc
7261 actgtgttgc ccaggctggt ttctaactcc tggcctcagc ctctcaaagt gctgggatta
7321 taggcatgag agggaacctc agagagaaca tctgtagccc attgctcttc aggggactca
7381 ttttaaaccc ttctcaagaa ggttgtcttt taatttttaa tttttatggg tatacaggtg
7441 tatatattta tgggttatat gagatgtttt gatacaggca tacaatgtgt aataatcata
7501 tcagggtgtg tggggtatcc attaccttaa gcatttatcc tttgtgttat aaacagtcca
7561 attatactgt tagttgtttt acaatataca attaaattat tttttgctat agtcacgttg
7621 gtgtgctagc aaatactagg tcttattcag aaggttgtct ttaaataaat cttaatttga
7681 aaaaaaaagt ttggtaatgg gttcatttgc actgaaagag ttttcttctt tttatttgag
7741 aagacaacaa atacatgaaa ttagattaat gtctccaaga catcagatca tttcatttgt
7801 atatgttttg gtatgtttta ctaaaagata aggactcatc ctttttaaac acaattgcag
CDS 3
7861 tgccatcatc acaccttcaa aagtatccac aataataatt ttagaaatat tttaaaatga
(8053…8143)
7921 tacaaataat gtttctggat gaaccttgag tgctgcccac cttacctcta cttttcccct
7981 aggcaatctt tagtattgca gatctaatag tatattatta ctaaaagtaa gcatgctttc
8041 tcttcttatt tctagtggcc ctgttccagt attggttatg agtcttctgt tcatcgcttc
8101 tgtatttatg ttgcacattt ggggcaagta cactcgttcg tagattcagt tacatccatc
8161 tgtcatctga agaaggagga aaaaacccaa catttcttgg accaaaagta tagtgactat
Exón 4
8221 ctgttcatga gagaaatttt ctgtaagctt gctgttttac aggggattta tcaataattg (8056…8334)
8281 attttgagga atcagttttt ttctatggct aataaacttt ttaattcact tata

Se puede sacar qué porcentaje del gen es ARNm, en este caso sería: (560/8,334) x 100 =

6,7% Porcentaje de ARNm en el gen.

PARTES DEL ARNm

  Celeste: 5’ UTR región no codificante

 Amarillo: CDS (Secuencia que se va a traducir)

 Verde: Región reguladora

 Rojo: 3’ UTR (incluyendo la zona verde)

 Fucsia: Inicio de la cola Poli-A

Resultados de la secuencia genómica de SEC61B

En genética, el porcentaje GC es una característica del genoma de un organismo o de

cualquier pedazo de ADN o ARN. G y C denotan guanina y citosina, respectivamente.

Expresado generalmente como porcentaje, representa la cantidad de pares Guanina y

Citosina en la molécula de ADN o genoma que está siendo investigado.

Tabla 1. Características de los exones del gen SEC61B de Homo sapiens

Exón Rango (pb) Tamaño Primero Último %GC

(pb) nucleótido nucleótido
(5’3’) (5’3’)
01 1-85 85 A G 60.98%
02 265-362 97 G G 69.44%
03 5618-5719 101 A T 58.59%
04 8056-8334 278 T A 35.56%

Tabla 2. Características de los intrones del gen SEC61B de Homo sapiens

Intron Rango (pb) Tamaño Primero Último

(pb) nucleótido nucleótido
(5’3’) (5’3’)
01 86-264 85 A G
02 363-5617 5254 G T
03 5720-8055 2335 G T