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Assignment Bioinformatics
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Muhammad Bilal
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(17-arid-2093)

BS BCH 5th Morning

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Phoenix dactylifera cultivar Shakri ATP
synthase beta subunit gene, partial cds;
plastid
ACCESSION KT781688
VERSION KT781688.1
SOURCE plastid Phoenix dactylifera (date palm)
ORGANISM Phoenix dactylifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Arecaceae; Coryphoideae;
Phoeniceae; Phoenix.
REFERENCE 1 (bases 1 to 625)
AUTHORS Faqir N., Hyder, M.Z., Muhammad, A., Shehzad, A., Rahman,
H. and Ali, G.M.
TITLE Genetic, biochemical and morphological evaluation of seven dates
Palm cultivars from Pakistan
TITLE Direct Submission
JOURNAL Submitted (18-SEP-2015) Plant Genomics and Biotechnology, National
Agriculture Research Center, Chak Shehzad, Park Road, Islamabad
45550, Pakistan

CDS <1...>625
/codon_start=3
/transl_table=11
/product="ATP synthase beta subunit"
/protein_id="ALO75645.1"
/translation="
GLMRGMEVIDTGAPLSVPVGGATLGRIFNVLGEPVDNLGPVDTR
TTSPIHRSAPAFIQLDTKLSIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMEL
INNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVINEKNIAESKVALVYGQMNEPP
GARMRVGLTALTMAEYFRDVNEQDVLLFIDNIFRFVQAGSEVSALLG"
ORIGIN
Fasta sequence
1 atgggctgat gagaggaatg gaagtgattg acacgggagc tcctctaagt gttccagtcg
61 gcggagctac tctcggacga attttcaacg ttcttgggga acctgttgat aatttaggtc
121 ctgtagatac tcgtacaaca tctcctattc atagatctgc gcctgccttt atacagttag
181 atacgaaatt atcaatcttt gaaacaggga ttaaagtggt ggatctttta gctccttatc
241 gccgtggagg aaaaatcgga ctatttgggg gagctggagt gggtaaaaca gtactcatca
301 tggaattgat caataacatt gccaaagctc atggaggcgt atccgtattt ggcggagtag
361 gcgaacgtac tcgtgaagga aatgatcttt acatggaaat gaaagaatcc ggagtgatta
421 atgaaaaaaa tattgcggaa tcaaaagtag ctctagtcta tggtcaaatg aatgaaccgc
481 cgggagctcg tatgagagtt ggtttaactg ccctaaccat ggcggaatat ttccgggatg
541 ttaatgaaca agacgtgctt ctattcatcg acaatatttt tcgtttcgtc caagcaggat
601 cagaagtatc cgccttatta gggag
//
Primer 1:
Length Start Stop Tm GC% Self complementarity
Sequence (5'->3') Template strand
Forward primer TGACACGGGAGCTCCTCTAA Plus 20 29 48 59.96 55.00
Reverse primer GAGTACGTTCGCCTACTCCG Minus 20 372 353 59.97 60.00
Product length 344