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Table Of Contents

Electrical hazards
Fire safety
Biological hazards
Safety equipment
BIOCHEMISTRY LABORATORY
Types of Anticoagulants
Ethylenediaminetetraacctic Acid (EDTA):
Sodium Fluoride:
Citrate:
Heparin:
Oxalates:
Iodoacetate:
Types of tubes:
General laboratory supplies
Glassware:
1. Pipettes:
2. Burettes:
3. Beakers:
4. Flasks:
Micropipette:
Cleaning of glassware:
Instructions for preparation of laboratory reports
CENTRIFUGE
PRINCIPLE OF CENTRIFUGATION
COMPONENTS OF A CENTRIFUGE
TYPES OF CENTRIFUGES
SPECTROPHOTOMETER
Light source:
Entrance & exit slits:
Monochromator:
Sample holder:
Detector:
Readout device:
Characteristics of wavelength:
The Electromagnetic Spectrum:
Beer-Lambert law
A = log (100 %) - log % T = 2- log % T = abc
BIOCHEMICAL CALCULATIONS
SI units:
Basic SI units:
Conversion factors:
Units of weight and volume:
Concentration units:
Conversion of traditional unit to SI unit and vice versa:
Molecular weight of come compounds
SPECIMEN COLLECTION
SERIAL DILUTIONS
(CALIBRATION CURVE)
BLOOD SUGAR ESTIMATION
Principle:
Conditions:
Normal value:
Clinical interpretation:
ORAL GLUCOSE TOLERANCE TEST (OGTT)
Standards for OGTT:
Observation:
Indications for OGGT
WHO criteria for the diagnosis of DM:
LIPID PROFILE
Plasma standing (appearance) test
PLASMA TOTAL CHOLESTEROL ESTIMATION
Introduction:
Sources of cholesterol:
Specimens:
Normal range:
Clinical significance:
SERUM TRIGLYCERIDES ESTIMATION
Specimen:
SERUM HDL-C ESTIMATION
Procedure:
Expected values:
Interpretation:
CALCULATION & ESTIMATION OF LDL-C
Friedewald calculation or Derived beta- quantification:
LDL-C estimation
Principle
Calculation:
Clinical interpretation
PLASMA TOTAL PROTEIN ESTIMATION
PLASMA ALBUMIN ESTIMATION
SERUM URIC ACID ESTIMATIOM
SERUM CALCIUM ESTIMATION
SERUM INORGANIC PHOSPHATE ESTIMATION
SERUM MAGNESIUM ESTIMATION
ASSESSMENT OF IRON STATUS
Lab markers
Reference Ranges & Analytical Techniques
Note
1. MEASUREMENT OF SERUM IRON
Specimen
Procedure
Clinical significance
2. MESTTREMENT OF TIBC
Transferrin Serum
Exogenous iron
Iron
Centrifugation
Calculation
3. MESTTREMENT OF UIBC
4. CALCULATION OF % SATURATION
CHEMICAL ANALYSIS OF CEREBROSPINAL FLUID
Determination of glucose and total protein
Function:
Composition:
Routine CSF laboratory investigations:
CSF GLUCOSE ESTIMATION
Contents
CSF TOTAL PROTEIN ESTIMATION
GLUCOSE 6 PHOSPHATE DEHYDROGENASE ESTIMATION
1 – Screening test:
Comment
2 – Estimation of erythrocytes and serum G6PD
Reagents
Preparation of solutions
Preparation of sample for estimation of erythrocytes G6PD activity
Procedure for estimation of Serum G6PD activity
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Clinical Biochemistry Practical I

Clinical Biochemistry Practical I

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Published by sama_omery

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Published by: sama_omery on May 21, 2011
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Shosho Biochemist added this note|
Good organization and explanation, the safety part is very useful. but I expected the names of the reagents and their contents with concetrations. It will be usefull if the preparation of the reagents is mentioned.
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