Welcome to Scribd. Sign in or start your free trial to enjoy unlimited e-books, audiobooks & documents.Find out more
Download
Standard view
Full view
of .
Look up keyword
Like this
2Activity
0 of .
Results for:
No results containing your search query
P. 1
7a203-mt----downstream processing

7a203-mt----downstream processing

Ratings: (0)|Views: 9|Likes:
Published by SRINIVASA RAO GANTA

More info:

Published by: SRINIVASA RAO GANTA on Sep 19, 2008
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

09/05/2014

pdf

text

original

NR
Code No: 7A203/MT
M.Tech. \u2013 II Semester Regular Examinations, September, 2008
DOWNSTREAM PROCESSING
(Biotechnology)
Time: 3hours
Max. Marks:60
Answer any FIVE questions
All questions carry equal marks
- - -
1.
Explain in detail about the characteristics of protein and various
steps to be adopted for recombinant protein purification.
2.

Write about different types of centrifuges and how they are scaled up. Compare the advantages disadvantages centrifugation with microfiltration.

3.a) Explain the chromatography equipment.
b) Write briefly about two methods of affinity chromatography.
4.
Write short notes on the following:
a)
Aqueous two phase extraction
b)
Ammonium sulphate precipitation.
5.
Write notes on the following:
a)
Ultra filtration
b)
Elution methods in ion exchange chromatography
c)
Super critical extraction.
6.

10g of BSA is eluted from an 80 litre Sephadex column which has a void fraction of 0.4. The concentration in the column peaks after 470 litres are eluted and the maximum concentration is 1.8% of that originally in the column. Estimate

a) The equilibrium constant for binding the albumin to the

sephadex
b) The number of stages in the column
c) The concentration profile in the column.

7.a) For disruption of baker\u2019s yeast, a homogenizer was found to give a yield of 49% when operated at 55 Mpa and 85% at 105 Mpa. Determine the number of passes required to obtain 85% disruption at 55 Mpa.

Contd\u2026.2.,

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->