Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword
Like this
2Activity
0 of .
Results for:
No results containing your search query
P. 1
Leo Rouhiainen

Leo Rouhiainen

Ratings: (0)|Views: 914|Likes:
Published by api-27019823

More info:

Published by: api-27019823 on Oct 18, 2008
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

03/18/2014

pdf

text

original

Characterization ofAnabaena cyanobacteria: repeated sequences and
genes involved in biosynthesis of microcystins and anabaenopeptilides
LEO ROUHIAINEN

Division of Microbiology
Department of Applied Chemistry and Microbiology
Viikki Biocenter, University of Helsinki
Finland

To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public criticism in the Auditorium 3 at the Viikki Infocenter (Viikinkaari 11) of the University of Helsinki, on October the 22th 2004 at 12 o\u2019clock noon.

Helsinki 2004
Academic Dissertation in Microbiology
2
Supervisors:Academy Prof. Kaarina Sivonen
Department of Applied Chemistry and Microbiology
University of Helsinki, Finland
Reviewers:

Academy Prof. Eva-Mari Aro
Department of Biology
University of Turku, Finland

Priv.-Doz. Dr. Hans von D\u00f6hren
Institute of Chemistry
Technical University Berlin, Germany

Opponent:

Prof. Pekka M\u00e4nts\u00e4l\u00e4
Department of Biochemistry and Food Chemistry
University of Turku, Finland

Printed:
Yliopistopaino 2004, Helsinki, Finland
Layout:
Otso Koski
ISSN
1239-9469
ISBN
952-10-2045-8 printed version
ISBN
952-10-2046-6 pdf version, http://ethesis.helsinki.\ue000
e-mail:
Leo.Rouhiainen@Helsinki.Fi
Front cover:
Anabaena cyanobacterium (photo by Anne Rantala)
3
Table of Contents

List of original articles.......................................................................................................... 4 The author\u2019s contribution in the articles............................................................................... 4 Abbreviations........................................................................................................................ 5

Abstract................................................................................................................................. 6
Tiivistelm\u00e4 (Abstract in Finnish).......................................................................................... 7
1. Introduction....................................................................................................................... 9
1.1 Cyanobacteria.............................................................................................................. 9
1.2 Cyanobacterial nonribosomal peptides..................................................................... 12
1.3 Biosynthesis of nonribosomal peptides..................................................................... 22
1.3.1 Nonribosomal peptide synthetases..................................................................... 22
1.3.2 Modular structure of peptide synthetases........................................................... 25
1.3.3 Substrate speci\ue000city of peptide synthetases....................................................... 25
1.3.4 Colinearity between peptide synthetases and their products.............................. 26
1.4 Biosynthesis of polyketides...................................................................................... 26
1.4.1 Types of bacterial polyketide synthases............................................................. 26
1.4.2 Bacterial mixed nonribosomal peptide synthetases/polyketide synthases......... 29
2. Aims of the present study................................................................................................ 30
3. Materials and Methods.................................................................................................... 31
4. Results............................................................................................................................. 34
4.1 Use of DNA probes to differentiate toxic cyanobacteria.......................................... 34
4.1.1 Repeated heptamer oligonucleotide as a probe.................................................. 34
4.1.2 Detection of the producers of microcystins and other nonribosomal peptides
with molecular probes and the polymerase chain reaction (PCR)............................. 34
4.2 Biosynthetic gene cluster of the anabaenopeptilides fromAnabaena 90................. 35
4.3 Genes for biosynthesis of the microcystins fromAnabaena 90................................ 36
5. Discussion....................................................................................................................... 38
6. Conclusions..................................................................................................................... 42

7. Acknowledgements......................................................................................................... 43
8. References....................................................................................................................... 44

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->