Group members

Salmiza bt Zainal Abidin D20091035074 Erdayu bt Sahar D20091035078 Hajah Sanita bt Mohd Satar D20091035086 Wan Jien D20091035127

Norharyan Noor Phua Pui

Wan Ting D20091034840

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1. Preparation of standard reference

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2. The effect of substrate concentration

Data analysis

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3. The effect of temperature

Data analysis

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Compare and discuss the velocity of the amylase activity at different temperature

At 0-20C enzymes work slowly 37C enzymes work most effectively at body temperature. 60-80C enzymes are denatured and become ineffective.

At At

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What is the optimum temperature for amylase activity? The optimum temperature for amylase activity is at 37C

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4. The effect of pH
Data Analysis

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Compare and discuss the velocity for each of the pH test

As

the pH increasing from 4 to 6, the velocity also increase. pH at 7 to 10, the velocity is decrease. in pH lead to the breaking of ionic bonds that hold the tertiary structure of the enzyme in place. So, the enzyme begins to lose its functional shape and the substrate will no longer fit to it, the enzyme is said to be denatured.

When

Changes

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What is the optimum pH for amylase activity?

The

optimum pH for amylase activity is 6

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