Reed & Muench Calculator

1. Enter the starting dilution 2. Enter the dilution factor 3. Enter the volume tested per well This is your calculated dilution series: 4. Enter the total # of wells examined per dilution 5. Enter the # of positive wells for each dilution These values are calculated automatically: initial dilution: dilution factor: ml: total wells: positive wells: negative wells: cum pos: cum neg: % infected: prop dist: TCID50: TCID50/ml: 0.1 10 0.1 1.00E-01 8 8 0 35 0 100.00 0

Created November 20, 2004 by Brett D. Lindenbach, PhD Modified January 27, 2008

1.00E-02 8 8 0 27 0 100.00 0

1.00E-03 8 8 0 19 0 100.00 0

6. This is your TCID50 7. This is your TCID50/ml

1.00E-04 8 8 0 11 0 100.00 0.8 1.58E-05 6.31E+05

1.00E-05 8 3 5 3 5 37.50 -0.33333333

1.00E-06 8 0 8 0 13 0.00 0

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