Basic Stock Solutions

Ampicillin (100mg/ml) APS (10%) ATP, 10mM 1 gm in 10ml dH2O, alliquot 1ml/tube, store -20C 18.15mg Na2ATP (mw 605)/3ml dH2O add 30 l 1M Tris pH 8 alliquot 300l/tube, store -20C

**mix on ic

Blue Juice (10X loading dye) 50 ml glycerol 2 ml 50X TAE 10 ml 10% SDS 2 ml 10% BPB (bromophenol blue) 2 ml 10% XC (Xye.ene cyanol) 43 ml dH2O

or 2 ml 10 mM Cresol Red or 2 ml 10 mM Tartrazine yellow

BSA, 10mg/ml dH2O, DEPC

sterile filter, alliquot 1ml/tube, store -20C add 500 l DEPC/500ml dH2O, mix well wait 12-24 hrs, autoclave 20 min. dissolve at 37C, on shaker, store in dark 8.62ml conc. HCl (11.6M)/100ml dH20

**mix on ice

Ethidium Br, 10mg/ml HCl, 1N

Proteinase K, 10mg/ml

add 10ml dH2O to a 100mg bottle of Prot. K add 5l 1N HCl alliquot 1ml/tube, store -20C 100mg RNase A, 100l 1M tris 7.5/10ml dH2O heat at 80C for 5 min. alliquot 1ml/tube, store -20C 200 gm SDS 4 ml 0.5 M EDTA 200 ml 1M Tris 7.5 76.4 mg spermidine (mw 254.6)/3ml dH2O alliquot 300l/tube, store -20C 104.5 mg spermine (mw 348.2)/3ml dH2O alliquot 300l/tube, store -20C 1753 gm NaCl, =3 M final conc. 882 gm Na3 Citrate, =0.3 M final conc. dH2O to 10 L, pH 7.0 with 1 N HCl (pH paper) 250 l 1 Tris 7.5 40 l 1M MgCl2 10 l 1M DTT 100 l 10mM ATP 25 l 100mM spermidine 575 l dH2O alliquot 50l/tube, store -20C 2420 gm Tris base (mw 121.1) 1361 gm NaOAc.3H2O 190 gm Na2EDTA 8 L dH2O, pH to 7.2-7.5 with 1062 ml glacial HOAc fill to 10 L with dH2o 500gm +210 ml dH2O 500 gm phenol add 200 ml 1M Tris 7.5 30 ml dH2O dissolve at 37C add 25 ml m-cresol 1 ml 2-mercaptoethanol 500 mg 8-hydroxyquinoline store at 4C

**mix on ice

RNase A, 10mg/ml

SET, 10X

Spermidine, 100mM Spermine, 100mM SSC, 20X

T4 Ligase buffer

**mix on ice

TAE, 50X

NH4OAc (Soln. 4) Phenol