( )

( )

(scientific name) Homo sapiens

5


1.
1.1
1.2
1.3
1.4
1.5
1.6
1.7 (Abstract)
1.8
2

 ( )

1.9
1.10
1.11
1.12 ()
1.13
2. 5
1
2
3
4
5
3.
4.
5.

1.1
1.2
1.3
1.4
1.5
1.6
3

 ( )

1.7 (Abstract)
1.8
1.9
1.10
1.11
1.12 ()
1.13
.

Candida oleophila
2554

(Cordia New 16)

 ( )

2 ( )

(Cordia New 22)

(Cordia New 22)

(Cordia New 20)

..........(Cordia New 18)

2554
( )
(Cordia New 18)
5

 ( )

3 ( )
)
3.81 .. 1.5

Candida oleophila

Biological Control of Anthracnose in Postharvested Guava

by Yeasts Candida oleophila

3.81 ..
1.5

2.54
..

2554
( )

3.5 .. 1.4

 ( )

..
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2.54 ..

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2.54 .. 1

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................................................................................

...................................................................

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...................................................................

................................

(Cordia New 16 )

..........................................................................................................................................................
..................................................................................................................................................(
Cordia New 16 )
........................................................................................................................................................................
........................................................................................................................................

 ( )

Candida oleophila

.
.

2554

(anthracnose) Colletotrichum gloeosporioides
4 Candida oleophila R13 Candida musae R6
Issatchenkia orientalis ER1 Candida quercitrusa L2

PDA
C. oliophilus R13
I. orientalis ER1 85.84 5106
/ C. musae R6 C. quercitrusa L2 (p < 0.05)
R13
76.88 ER1 R6 L2 2 R13
ER1 C. gloeosporioides R13
ER1
R13 ER1
C. gloeosporioides
10

 ( )

/ Colletotrichum gloeosporioides/ Candida oleophila/ Issatchenkia

orientalis

11

 ( )

( )
...................................................................................................................

Title

Researchers ................................................................................
Author

................................................................................

Program

................................................................................

Major Advisor .............................................................................

Co-Advisor

.............................................................................

Degree

.............................................................................

Program Study ............................................................................

University

.............................................................................

Academic Year................................

(Cordia New 16 )
Abstract
..........................................................................................................................................................
........................................................................................................................................................................
........................................................................................................................................................................
........................................................................................................................ ( Cordia New
16 )......................................

12

 ( )

Title

Biological Control of Anthracnose in Postharvested Guava by Yeasts

Candida oleophila

Researchers
Advisor
Co-Advisor
Degree
Program Study
Faculty
University
Academic year

Miss Supranee Yingprasert

Mr. Teeradate Sapwirai
Assist Prof. Dr. Arun Chanchaichaovivat
Assoc. Prof. Dr. Wantanee Sawangarom
Bachelor of Science
Applied Biology
Science and Technology
Bansomdejchaopraya Rajabhat University
2011

Abstract
The objectives of this research were to examine and compare the efficacies of
antagonistic yeasts, i.e. Candida oleophila R13, Candida musae R6, Issatchenkia orientalis
ER1 and Candida quercitrusa L2 for controlling chilli anthracnose caused by Colletotrichum
gloeosporioides. The interaction between yeasts and mold was also observed. The efficacies
of yeast antagonists were determined by well test method and in vivo test (on chilli fruit). In
well test, the results revealed that C. oliophilus R13 has efficacy in controlling the disease
equally to I. orientalis ER1 (85.84 %). In vivo test, yeast strain R13 showed higher efficacy
(76.88 %) than those yeast strains, ER1, R6, and L2, respectively (p < 0.05). Under
microscopic condition, the attachment between strain R13 cells and fungal hyphae was
observed, but this attachment ability did not present in yeast strain ER1. Therefore, yeast
strains R13 and ER1 have the potential to use as biocontrol agents for controlling chilli
anthracnose caused by C. gloeosporioides.

13

 ( )

Keywords: Anthracnose/ Chilli fruit/ Colletotrichum gloeosporioides/ Candida oleophila/

Issatchenkia orientalis

14

 ( )

3.81 .. 1.5
1.8

Candida oleophila
1.9

1.10

.
.
1.11

1.12

2554

3.81 ..

1.5

(anthracnose) Colletotrichum gloeosporioides
4 Candida oleophila R13
Candida musae R6 Issatchenkia orientalis ER1 Candida quercitrusa L2

PDA
Candida
oleophila R13 I. orientalis ER1
85.84 5106 / C.
musae R6 C. quercitrusa L2 (p < 0.05)
R13 76.88
ER1 R6 L2 2 R13 ER1 C.
gloeosporioides R13
ER1
R13 ER1
C. gloeosporioides

2.54 ..

: / Colletotrichum gloeosporioides/ Candida oleophila/ Issatchenkia

orientalis

2.54 .. 1

15

 ( )

3.81 .. 1.5

(Cordia New 20 )

3.81 ..

.............
(Cordia New 16
)..............................................................................................................
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6.
6.1
6.2

2.54 ..

20 2554
2.54 .. 1

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Crodia new 16 .

17

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10

()
()
1
1.1
1.2
1.3
1.4
2
2.1 ()
2.2 ()
2.2.1 ()
2.2.2 ()
2.2.3 ()
2.3 ()
2.3.1 ()
2.3.2 ()

I
II
III
IV
VI
VII
VIII
VIIII
1
1
3
3
4
7
7
15
16
17
18
19
20
21

18

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( )

23

3.1

23

3.2

25

33

4.1 ( )

33

4.2 ( )

38

4.3 ( )

39

4.4 ( )

40

52

5.1

60

5.2

56

5.3

60

61

70

71

74

76

80

19

 ( )

11

1.1
1.2
1.3
2.1
2.2
2.3
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()
()
()
()
()
()
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()
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()
()
()
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()

17
20
22
25
26
27
28
29
50
52
53
54
55
56
57

20

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12

1.1 ()
1.2 ()
2.1 ()
2.2 ()
2.3 ()
3.1 ()
3.2 ()
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7
8
15
16
50
58
59
60
61
63

21

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13

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22

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23

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24

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25

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29

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30

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31

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32

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33

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36

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37

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( )
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38

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4.1 (%) 0.1 /
7 A B D F I J R

A
OD600
1
2
3
4
5
6
7

0.119
0.104
0.069
0.061
0.052
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0.046

B
%

37.70
45.55
63.87
68.06
72.77
73.30
75.92

OD600
0.180
0.138
0.081
0.060
0.036
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D
%

5.76
27.75
57.59
68.59
81.15
81.68
87.96

OD600
0.134
0.096
0.076
0.068
0.064
0.048
0.037

29.84
49.74
60.21
64.40
66.49
74.87
80.63

F
OD600
%

0.108 43.46
0.074 61.26
0.060 68.59
0.044 77.37
0.042 78.01
0.034 82.20
0.022 88.48

I
OD600
0.090
0.064
0.051
0.048
0.041
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0.020

J
%

52.89
66.49
73.30
74.87
78.53
81.68
89.53

OD600
0.121
0.086
0.063
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0.041
0.030

R
%

36.65
54.97
67.02
69.11
70.16
78.53
84.29

OD600
0.048
0.044
0.033
0.032
0.021
0.015
0.012

74.87
76.96
82.72
83.25
89.01
92.15
93.72

39

 ( )

21

(BOD : N :
P) 100 1.1 0.2 30-50 o -
6.6-7.4



6 ( 2.5)

2.5
(Clisso, 2010: 3)

40

 ( )

1

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(2.1)

41

 ( )

3.81 .. 1.5

5

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.................................................................................................
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42

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43

 ( )

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44

 ( )

23

1. (in-text citation)
-
-
(,) 1

..........( )..........( , 2551)

1. 1

( , 2551)
(Bamforth, 2005)

( , 2554: 56-58)
2. 2

( , 2550)
(Alexopoulous & Mims, 1979: 58)
3. 3

(El-Mansi, et al., 2007)
( , 2551)
4.

(, 2548)
(The National Health Museum, 2009)
5.

45

 ( )

(;)
(Arras et al., 2002; Druvefors, 2004; Fravel, 2005)
6.
cited in ( )
( , 2550 , 2553: 32)
(Druvefors, 2004 cited in Chanchaichaovivat et al., 2007: 21)

Mehrotra and Aggawal (2003)

.....( Chanchaichaovivat et al., 2010)
2. (reference citation)

(
) APA (American Psychological Association)

(last name) (,)
(first name) (.)
(middle name)
,

Mary Brown

Brown, M.
Robert Metzenberg Hammer
Hammer, R.M.

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Cordia New (Bold) 20 1
Cordia New 16
46

 ( )

1.25 0.5 3.81 2.54 3.81

2.54

47

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(Cordia New 24 )

48

 ( )

24

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50

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25

Cordia New (Bold) 20
1
Cordia New 16 1.25 0.5
3.81 2.54 3.81 2.54

1. (
)
2. (Questionnaire
(Check List) (Inventory) (Test)
3. (Case Study)
(Content Analysis)
4.
5.

6. (Directory)

51

 ( )

(Cordia New 24 )

52

 ( )

(Cordia New 24 )

53

 ( )

26

0.1-1.2 / MS
7 1-12
1
0.1 / A B D F I J C R 7
Sum of
df
Mean
F
Sig.
squares
square
Between
643.747
6
107.291
4.464
.010
Groups
336.474
14
24.034
Within Groups
980.221
20
Total
2 0.1
/ A B D F I J C R 7
A
B
D
F
I

A
12.0400* 4.7100 12.5600* 13.6100*
B
7.3300 0.5200 1.5700
D
7.8500 8.9000*
F
1.0500
I
J
* 0.05

8.370
3.6700
3.6600
4.1900
5.2400
-

17.7667*
5.7267
13.0567*
5.2067
4.1567
9.3967*

54

 ( )

()
..
()

()
Cordia New (Bold) 20
Cordia New 16
0.5 1.25
(page setup)

55

 ( )

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56

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27
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..........................................

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.
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........
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.

57