321

By: Amal Alghamdi


.Mixed Culture

Antagonism
.antibiosis
:

.Antibiosis

By: Amal Alghamdi


:



.Antibiotics
: :

.
:
:

By: Amal Alghamdi


Effect of Antibiotics on Bacterial Physiology

By: Amal Alghamdi


Mode of Action
:
( Bactiricin Cephalosporin Penicillin:
.)Vancomycin
(Tetracyclins Cholramphenicol :
.)Gentamycin Erythromycin Streptomycins
(DNA)Quinolones :
( RNA)Rifampin:
()Sulfanilamide :
( )Bolymyxin B :

By: Amal Alghamdi

 Mode of Action

By: Amal Alghamdi


Antibiotic Sensitivity Test

.

.
.

24
Minimum
.)MIC( Inhibitory Concentration
By: Amal Alghamdi




.
:
.1 Disk diffusion method
Bauer - Kirby method
.2 Broth dilution method
. Agar dilution method
.3 Gradient Plate Method
E-test
).(Epsilometer
By: Amal Alghamdi



-1
Disk Diffusion Method

:
.1 Unidisk
.2 Multidisc

By: Amal Alghamdi

 -1 Disk Diffusion Method

:

( Mueller-Hinton media Blood
)Agar media
.
:
-1 .
-2 swab 3 -
- .
-3
.
-4 37 24- 18.
-5 Inhibition zone
.
By: Amal Alghamdi

 susceptibility
Intermediate Resistant

By: Amal Alghamdi



- 2 ):E-test (Epsilometer
15

.

.
.AST

By: Amal Alghamdi

 :E-test

( MIC
/) MIC
.
By: Amal Alghamdi

Vancomycin E-test :
Staphylococcus aureus

http://commons.wikimedia.org/wiki/File:Etest_Vancomycin_S_aureus.jpg
Size of this preview: 800 338 pixels
By: Amal Alghamdi

: Inhibition zones
.

: Etest Bio-Stat
MIC
.

Pictures, Professor D. Taylor, University of Glasgow :

By: Amal Alghamdi

Amoxycillin

Minimum Inhibitory Concentration of Amoxycillin to
different types of Bacteria by Gradient Plate Method

By: Amal Alghamdi


Amoxycillin
: Amoxycillin
.MIC
: 1
%70
Amoxycillin.
.Mueller-Hinton media :

By: Amal Alghamdi


Amoxycillin
:
-1 45.
-2 20
( ).
(.)1

-3 2.7
10
.
-4 1 19
45.
By: Amal Alghamdi


Amoxycillin
/ :
-4

:
(.)g/mL

Gradient Plate
( %0 .) %100
By: Amal Alghamdi


Amoxycillin
/ :

-5 0,5

37 24-18.
-6
Inhibition Zone
.
-7 MIC
.
By: Amal Alghamdi


Amoxycillin
0
%

50
%

100
%

Bacterial Loan

Inhibition Zone

By: Amal Alghamdi


Amoxycillin
:
.i

.ii
.iii
.

By: Amal Alghamdi