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    Liquid chromatography uses a liquid mobile phase and is suitable for separating non-volatile compounds, operating at high temperatures and pressures with short, wide columns. Gas chromatography uses a gas mobile phase and is suitable for separating volatile compounds, operating at lower pressures with long, narrow capillary columns. Both techniques separate samples based on interactions with stationary and mobile phases, but gas chromatography can separate a wider range of molecular weights and requires volatile, low boiling point solvents to prepare samples.

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    Liquid chromatography uses a liquid mobile phase and is suitable for separating non-volatile compounds, operating at high temperatures and pressures with short, wide columns. Gas chromatography uses a gas mobile phase and is suitable for separating volatile compounds, operating at lower pressures with long, narrow capillary columns. Both techniques separate samples based on interactions with stationary and mobile phases, but gas chromatography can separate a wider range of molecular weights and requires volatile, low boiling point solvents to prepare samples.

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    2 views3 pages

    CA Assignment

    Uploaded by

    rshafizan
    Liquid chromatography uses a liquid mobile phase and is suitable for separating non-volatile compounds, operating at high temperatures and pressures with short, wide columns. Gas chromatography uses a gas mobile phase and is suitable for separating volatile compounds, operating at lower pressures with long, narrow capillary columns. Both techniques separate samples based on interactions with stationary and mobile phases, but gas chromatography can separate a wider range of molecular weights and requires volatile, low boiling point solvents to prepare samples.

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    Chemical Analysis Assignment Name ID Lecturer : Mohd.

    Ridzhuan Bin Jinal : 14097 : Asna Mohd Zain

    Comparison between Liquid Chromatography and Gas Chromatography


    ELEMENT Mobility Phase Volatility Operating Temperature Operating Pressure Column Separation Mechanism Sample Molecular Weight Sample Characteristic LIQUID CHROMATOGRAPHY Liquid Non-volatile compounds sample High High Short and wider Mobile phase is a sample carrier only. No theoretical upper limit for molecular weight. Sample must be filtered and be in same solvent as mobile phase for sample preparation. Based upon column inner diameter Most common UV-Vis, wide range of nondestructive detectors, 3dimensional detectors, sensitivity to fg (detector dependent) GAS CHROMATOGRAPHY Gas Volatile compounds sample High Low Capillary column Both stationary phase and mobile phase. Typically < 500 amu Solvent must be volatile and lower boiling point than analytes for sample preparation Typically 1 - 5 L Most common FID, universal to organic compounds

    Sample Size Detectors

    Chemical Analysis Assignment High Performance Liquid Chromatography (HPLC)

    Schematic of an HPLC instrument

    Picture of an HPLC instrument

    Chemical Analysis Assignment Gas chromatography schematic :

    Schematic of an GC instrument

    Picture of an GC instrument

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